Cannabidiol Activates Neuronal Precursor Genes in Human Gingival Mesenchymal Stromal Cells

Rajan Thangavelu Soundara, Sabrina Giacoppo, Domenico Scionti, Francesca Diomede, Gianpaolo Grassi, Federica Pollastro, Adriano Piattelli, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani

Research output: Contribution to journalArticle

Abstract

In the last years, mesenchymal stromal cells (MSCs) from oral tissues have received considerable interest in regenerative medicine since they can be obtained with minimal invasive procedure and exhibit immunomodulatory properties. This study was aimed to investigate whether in vitro pre-treatment of MSCs obtained from human gingiva (hGMSCs) with Cannabidiol (CBD), a cannabinoid component produced by the plant Cannabis sativa, may promote human gingiva derived MSCs to differentiate toward neuronal precursor cells. Specifically, we have treated the hGMSCs with CBD (5 µM) for 24 h in order to evaluate the expression of genes involved in cannabidiol signaling, cell proliferation, self-renewal and multipotency, and neural progenitor cells differentiation. Next generation sequencing (NGS) demonstrated that CBD activates genes associated with G protein coupled receptor signaling in hGMSCs. Genes involved in DNA replication, cell cycle, proliferation, and apoptosis were regulated. Moreover, genes associated with the biological process of neuronal progenitor cells (NCPs) proliferation, neuron differentiation, neurogenesis, and nervous system development were significantly modulated. From our results, we hypothesize that human gingiva-derived MSCs conditioned with CBD could represent a valid method for improving the hGMSCs phenotype and thus might be a potential therapeutic tool in the treatment of neurodegenerative diseases. J. Cell. Biochem. 9999: 1-16, 2016. © 2016 Wiley Periodicals, Inc.

Original languageEnglish
JournalJournal of Cellular Biochemistry
DOIs
Publication statusE-pub ahead of print - Dec 5 2016

Fingerprint

Cannabidiol
Mesenchymal Stromal Cells
Genes
Gingiva
Cell Proliferation
Cell proliferation
Stem Cells
Neurodegenerative diseases
Biological Phenomena
Plant Structures
Regenerative Medicine
Cannabinoids
Neurogenesis
Neurology
Cannabis
G-Protein-Coupled Receptors
DNA Replication
Neurodegenerative Diseases
Nervous System
Neurons

Keywords

  • Journal Article

Cite this

Soundara, R. T., Giacoppo, S., Scionti, D., Diomede, F., Grassi, G., Pollastro, F., ... Trubiani, O. (2016). Cannabidiol Activates Neuronal Precursor Genes in Human Gingival Mesenchymal Stromal Cells. Journal of Cellular Biochemistry. https://doi.org/10.1002/jcb.25815

Cannabidiol Activates Neuronal Precursor Genes in Human Gingival Mesenchymal Stromal Cells. / Soundara, Rajan Thangavelu; Giacoppo, Sabrina; Scionti, Domenico; Diomede, Francesca; Grassi, Gianpaolo; Pollastro, Federica; Piattelli, Adriano; Bramanti, Placido; Mazzon, Emanuela; Trubiani, Oriana.

In: Journal of Cellular Biochemistry, 05.12.2016.

Research output: Contribution to journalArticle

Soundara, Rajan Thangavelu ; Giacoppo, Sabrina ; Scionti, Domenico ; Diomede, Francesca ; Grassi, Gianpaolo ; Pollastro, Federica ; Piattelli, Adriano ; Bramanti, Placido ; Mazzon, Emanuela ; Trubiani, Oriana. / Cannabidiol Activates Neuronal Precursor Genes in Human Gingival Mesenchymal Stromal Cells. In: Journal of Cellular Biochemistry. 2016.
@article{2033e539bbe142fe846911ff50edc6f0,
title = "Cannabidiol Activates Neuronal Precursor Genes in Human Gingival Mesenchymal Stromal Cells",
abstract = "In the last years, mesenchymal stromal cells (MSCs) from oral tissues have received considerable interest in regenerative medicine since they can be obtained with minimal invasive procedure and exhibit immunomodulatory properties. This study was aimed to investigate whether in vitro pre-treatment of MSCs obtained from human gingiva (hGMSCs) with Cannabidiol (CBD), a cannabinoid component produced by the plant Cannabis sativa, may promote human gingiva derived MSCs to differentiate toward neuronal precursor cells. Specifically, we have treated the hGMSCs with CBD (5 µM) for 24 h in order to evaluate the expression of genes involved in cannabidiol signaling, cell proliferation, self-renewal and multipotency, and neural progenitor cells differentiation. Next generation sequencing (NGS) demonstrated that CBD activates genes associated with G protein coupled receptor signaling in hGMSCs. Genes involved in DNA replication, cell cycle, proliferation, and apoptosis were regulated. Moreover, genes associated with the biological process of neuronal progenitor cells (NCPs) proliferation, neuron differentiation, neurogenesis, and nervous system development were significantly modulated. From our results, we hypothesize that human gingiva-derived MSCs conditioned with CBD could represent a valid method for improving the hGMSCs phenotype and thus might be a potential therapeutic tool in the treatment of neurodegenerative diseases. J. Cell. Biochem. 9999: 1-16, 2016. {\circledC} 2016 Wiley Periodicals, Inc.",
keywords = "Journal Article",
author = "Soundara, {Rajan Thangavelu} and Sabrina Giacoppo and Domenico Scionti and Francesca Diomede and Gianpaolo Grassi and Federica Pollastro and Adriano Piattelli and Placido Bramanti and Emanuela Mazzon and Oriana Trubiani",
note = "{\circledC} 2016 Wiley Periodicals, Inc.",
year = "2016",
month = "12",
day = "5",
doi = "10.1002/jcb.25815",
language = "English",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",

}

TY - JOUR

T1 - Cannabidiol Activates Neuronal Precursor Genes in Human Gingival Mesenchymal Stromal Cells

AU - Soundara, Rajan Thangavelu

AU - Giacoppo, Sabrina

AU - Scionti, Domenico

AU - Diomede, Francesca

AU - Grassi, Gianpaolo

AU - Pollastro, Federica

AU - Piattelli, Adriano

AU - Bramanti, Placido

AU - Mazzon, Emanuela

AU - Trubiani, Oriana

N1 - © 2016 Wiley Periodicals, Inc.

PY - 2016/12/5

Y1 - 2016/12/5

N2 - In the last years, mesenchymal stromal cells (MSCs) from oral tissues have received considerable interest in regenerative medicine since they can be obtained with minimal invasive procedure and exhibit immunomodulatory properties. This study was aimed to investigate whether in vitro pre-treatment of MSCs obtained from human gingiva (hGMSCs) with Cannabidiol (CBD), a cannabinoid component produced by the plant Cannabis sativa, may promote human gingiva derived MSCs to differentiate toward neuronal precursor cells. Specifically, we have treated the hGMSCs with CBD (5 µM) for 24 h in order to evaluate the expression of genes involved in cannabidiol signaling, cell proliferation, self-renewal and multipotency, and neural progenitor cells differentiation. Next generation sequencing (NGS) demonstrated that CBD activates genes associated with G protein coupled receptor signaling in hGMSCs. Genes involved in DNA replication, cell cycle, proliferation, and apoptosis were regulated. Moreover, genes associated with the biological process of neuronal progenitor cells (NCPs) proliferation, neuron differentiation, neurogenesis, and nervous system development were significantly modulated. From our results, we hypothesize that human gingiva-derived MSCs conditioned with CBD could represent a valid method for improving the hGMSCs phenotype and thus might be a potential therapeutic tool in the treatment of neurodegenerative diseases. J. Cell. Biochem. 9999: 1-16, 2016. © 2016 Wiley Periodicals, Inc.

AB - In the last years, mesenchymal stromal cells (MSCs) from oral tissues have received considerable interest in regenerative medicine since they can be obtained with minimal invasive procedure and exhibit immunomodulatory properties. This study was aimed to investigate whether in vitro pre-treatment of MSCs obtained from human gingiva (hGMSCs) with Cannabidiol (CBD), a cannabinoid component produced by the plant Cannabis sativa, may promote human gingiva derived MSCs to differentiate toward neuronal precursor cells. Specifically, we have treated the hGMSCs with CBD (5 µM) for 24 h in order to evaluate the expression of genes involved in cannabidiol signaling, cell proliferation, self-renewal and multipotency, and neural progenitor cells differentiation. Next generation sequencing (NGS) demonstrated that CBD activates genes associated with G protein coupled receptor signaling in hGMSCs. Genes involved in DNA replication, cell cycle, proliferation, and apoptosis were regulated. Moreover, genes associated with the biological process of neuronal progenitor cells (NCPs) proliferation, neuron differentiation, neurogenesis, and nervous system development were significantly modulated. From our results, we hypothesize that human gingiva-derived MSCs conditioned with CBD could represent a valid method for improving the hGMSCs phenotype and thus might be a potential therapeutic tool in the treatment of neurodegenerative diseases. J. Cell. Biochem. 9999: 1-16, 2016. © 2016 Wiley Periodicals, Inc.

KW - Journal Article

U2 - 10.1002/jcb.25815

DO - 10.1002/jcb.25815

M3 - Article

C2 - 27918106

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

ER -