Cannabinoid Receptor-1 Up-regulation in Azoxymethane (AOM)-treated Mice After Dietary Treatment with Quercetin

Research output: Contribution to journalArticle

Abstract

BACKGROUND/AIM: The expression of cannabinoid receptor-1 (CB1-R) seems to be modulated by bioactive natural components such as the flavonoid quercetin. The aim of this study was to determine in an animal model of induced-colon cancer, whether quercetin inhibits colon carcinogenesis through changes in the expression of CB1-R.

MATERIALS AND METHODS: C57BL/6J male mice were randomly assigned to standard diet or experimental diet supplemented with 0.5% quercetin. Azoxymethane (AOM) (10 mg/kg body weight) or saline solution (PBS) was intraperitoneally injected, once weekly for 6 weeks.

RESULTS: The diet supplemented with quercetin induced CB1-R gene expression and protein, inhibiting the protein levels of STAT3 and p-STAT3 (both mediators of cell proliferation). Dietary quercetin also caused a significant increase in Bax/Bcl2 ratio protein expression.

CONCLUSION: The anti-proliferative and pro-apoptotic effects of quercetin in AOM-treated mice are mediated by induction of the protein and gene expression levels of CB1-R.

Original languageEnglish
Pages (from-to)4485-4491
Number of pages7
JournalAnticancer Research
Volume38
Issue number8
DOIs
Publication statusPublished - Aug 2018

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Azoxymethane
Cannabinoid Receptors
Quercetin
Up-Regulation
Diet
vpr Genes
Gene Expression
Cannabinoid Receptor CB1
STAT3 Transcription Factor
Proteins
Flavonoids
Sodium Chloride
Colonic Neoplasms
Colon
Carcinogenesis
Animal Models
Body Weight
Cell Proliferation

Keywords

  • Animals
  • Apoptosis/drug effects
  • Azoxymethane/pharmacology
  • Carcinogenesis/drug effects
  • Cell Proliferation/drug effects
  • Colonic Neoplasms/drug therapy
  • Diet
  • Dietary Supplements
  • Flavonoids/pharmacology
  • Gene Expression/drug effects
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Proto-Oncogene Proteins c-bcl-2/metabolism
  • Quercetin/pharmacology
  • Receptor, Cannabinoid, CB1/metabolism
  • Up-Regulation/drug effects
  • bcl-2-Associated X Protein/metabolism

Cite this

@article{984f895ec4bd4bfbb0621d80860aa199,
title = "Cannabinoid Receptor-1 Up-regulation in Azoxymethane (AOM)-treated Mice After Dietary Treatment with Quercetin",
abstract = "BACKGROUND/AIM: The expression of cannabinoid receptor-1 (CB1-R) seems to be modulated by bioactive natural components such as the flavonoid quercetin. The aim of this study was to determine in an animal model of induced-colon cancer, whether quercetin inhibits colon carcinogenesis through changes in the expression of CB1-R.MATERIALS AND METHODS: C57BL/6J male mice were randomly assigned to standard diet or experimental diet supplemented with 0.5{\%} quercetin. Azoxymethane (AOM) (10 mg/kg body weight) or saline solution (PBS) was intraperitoneally injected, once weekly for 6 weeks.RESULTS: The diet supplemented with quercetin induced CB1-R gene expression and protein, inhibiting the protein levels of STAT3 and p-STAT3 (both mediators of cell proliferation). Dietary quercetin also caused a significant increase in Bax/Bcl2 ratio protein expression.CONCLUSION: The anti-proliferative and pro-apoptotic effects of quercetin in AOM-treated mice are mediated by induction of the protein and gene expression levels of CB1-R.",
keywords = "Animals, Apoptosis/drug effects, Azoxymethane/pharmacology, Carcinogenesis/drug effects, Cell Proliferation/drug effects, Colonic Neoplasms/drug therapy, Diet, Dietary Supplements, Flavonoids/pharmacology, Gene Expression/drug effects, Male, Mice, Mice, Inbred C57BL, Proto-Oncogene Proteins c-bcl-2/metabolism, Quercetin/pharmacology, Receptor, Cannabinoid, CB1/metabolism, Up-Regulation/drug effects, bcl-2-Associated X Protein/metabolism",
author = "Valeria Tutino and {DE Nunzio}, Valentina and Angela Tafaro and Giusy Bianco and Isabella Gigante and Scavo, {Maria Principia} and Rosalba D'Alessandro and Refolo, {Maria Grazia} and Caterina Messa and Caruso, {Maria Gabriella} and Maria Notarnicola",
note = "Copyright{\circledC} 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.",
year = "2018",
month = "8",
doi = "10.21873/anticanres.12752",
language = "English",
volume = "38",
pages = "4485--4491",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "8",

}

TY - JOUR

T1 - Cannabinoid Receptor-1 Up-regulation in Azoxymethane (AOM)-treated Mice After Dietary Treatment with Quercetin

AU - Tutino, Valeria

AU - DE Nunzio, Valentina

AU - Tafaro, Angela

AU - Bianco, Giusy

AU - Gigante, Isabella

AU - Scavo, Maria Principia

AU - D'Alessandro, Rosalba

AU - Refolo, Maria Grazia

AU - Messa, Caterina

AU - Caruso, Maria Gabriella

AU - Notarnicola, Maria

N1 - Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

PY - 2018/8

Y1 - 2018/8

N2 - BACKGROUND/AIM: The expression of cannabinoid receptor-1 (CB1-R) seems to be modulated by bioactive natural components such as the flavonoid quercetin. The aim of this study was to determine in an animal model of induced-colon cancer, whether quercetin inhibits colon carcinogenesis through changes in the expression of CB1-R.MATERIALS AND METHODS: C57BL/6J male mice were randomly assigned to standard diet or experimental diet supplemented with 0.5% quercetin. Azoxymethane (AOM) (10 mg/kg body weight) or saline solution (PBS) was intraperitoneally injected, once weekly for 6 weeks.RESULTS: The diet supplemented with quercetin induced CB1-R gene expression and protein, inhibiting the protein levels of STAT3 and p-STAT3 (both mediators of cell proliferation). Dietary quercetin also caused a significant increase in Bax/Bcl2 ratio protein expression.CONCLUSION: The anti-proliferative and pro-apoptotic effects of quercetin in AOM-treated mice are mediated by induction of the protein and gene expression levels of CB1-R.

AB - BACKGROUND/AIM: The expression of cannabinoid receptor-1 (CB1-R) seems to be modulated by bioactive natural components such as the flavonoid quercetin. The aim of this study was to determine in an animal model of induced-colon cancer, whether quercetin inhibits colon carcinogenesis through changes in the expression of CB1-R.MATERIALS AND METHODS: C57BL/6J male mice were randomly assigned to standard diet or experimental diet supplemented with 0.5% quercetin. Azoxymethane (AOM) (10 mg/kg body weight) or saline solution (PBS) was intraperitoneally injected, once weekly for 6 weeks.RESULTS: The diet supplemented with quercetin induced CB1-R gene expression and protein, inhibiting the protein levels of STAT3 and p-STAT3 (both mediators of cell proliferation). Dietary quercetin also caused a significant increase in Bax/Bcl2 ratio protein expression.CONCLUSION: The anti-proliferative and pro-apoptotic effects of quercetin in AOM-treated mice are mediated by induction of the protein and gene expression levels of CB1-R.

KW - Animals

KW - Apoptosis/drug effects

KW - Azoxymethane/pharmacology

KW - Carcinogenesis/drug effects

KW - Cell Proliferation/drug effects

KW - Colonic Neoplasms/drug therapy

KW - Diet

KW - Dietary Supplements

KW - Flavonoids/pharmacology

KW - Gene Expression/drug effects

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Proto-Oncogene Proteins c-bcl-2/metabolism

KW - Quercetin/pharmacology

KW - Receptor, Cannabinoid, CB1/metabolism

KW - Up-Regulation/drug effects

KW - bcl-2-Associated X Protein/metabolism

U2 - 10.21873/anticanres.12752

DO - 10.21873/anticanres.12752

M3 - Article

C2 - 30061214

VL - 38

SP - 4485

EP - 4491

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 8

ER -