Capillary electrophoresis of peptides and proteins in isoelectric buffers: An update

Pier Giorgio Righetti, Cecilia Gelfi, Alessandra Bossi, Erna Olivieri, Laura Castelletti, Barbara Verzola, Alexander V. Stoyanov

Research output: Contribution to journalArticlepeer-review

Abstract

Capillary electrophoresis in acidic, isoelectric buffers is a novel methodology allowing fast protein and peptide analysis in uncoated capillaries. Due to the low pH adopted and to the use of dynamic coating with cellulose derivatives, silanol ionization is essentially suppressed and little interaction of macromolecules with the untreated wall occurs. In addition, due to the low conductivity of quasi-stationary, isoelectric buffers, high-voltage gradients can be applied (up to 800 V/cm) permitting fast peptide analysis with a high resolving power due to minimal diffusional peak spreading. Four such buffers are here described: cysteic acid (Cys-A, p/1.85), iminodiacetic acid (IDA, p/2.23), aspartic acid (Asp, p/2.77) and glutamic acid (Glu, p/3.22). A number of applications are reported, ranging from food analysis to the study of folding/unfolding transitions of proteins.

Original languageEnglish
Pages (from-to)4046-4053
Number of pages8
JournalElectrophoresis
Volume21
Issue number18
DOIs
Publication statusPublished - 2000

Keywords

  • Capillary electrophoresis
  • Isoelectric buffers
  • Peptide analysis
  • Review

ASJC Scopus subject areas

  • Clinical Biochemistry

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