Capillary electrophoresis of peptides and proteins using isoelectric buffers.

P. G. Righetti, A. Bossi, C. Gelfi

Research output: Contribution to journalArticle

Abstract

Capillary electrophoresis in acidic isoelectric buffers is a novel methodology allowing fast protein and peptide analysis in uncoated capillaries. Due to the low pH adopted and to the use of dynamic coating with cellulose derivatives, silanol ionization is essentially suppressed and no interaction of macromolecules with the untreated wall ensues. In addition, because of the low conductivity of quasi-stationary isoelectric buffers, high voltage gradients can be applied (up to 800 V/cm), permitting fast peptide analysis with a high resolving power and minimal diffusional peak spreading.

Original languageEnglish
JournalCurrent Protocols in Protein Science
VolumeChapter 10
Publication statusPublished - May 2001

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Buffers
Cellulose derivatives
Peptides
Optical resolving power
Macromolecules
Cellulose
Ionization
Proteins
Coatings
Electric potential
silanol

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Capillary electrophoresis of peptides and proteins using isoelectric buffers. / Righetti, P. G.; Bossi, A.; Gelfi, C.

In: Current Protocols in Protein Science, Vol. Chapter 10, 05.2001.

Research output: Contribution to journalArticle

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