Abstract
Capillary electrophoresis in acidic isoelectric buffers is a novel methodology allowing fast protein and peptide analysis in uncoated capillaries. Due to the low pH adopted and to the use of dynamic coating with cellulose derivatives, silanol ionization is essentially suppressed and no interaction of macromolecules with the untreated wall ensues. In addition, because of the low conductivity of quasi-stationary isoelectric buffers, high voltage gradients can be applied (up to 800 V/cm), permitting fast peptide analysis with a high resolving power and minimal diffusional peak spreading.
| Original language | English |
|---|---|
| Journal | Current Protocols in Protein Science |
| Volume | Chapter 10 |
| Publication status | Published - May 2001 |
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ASJC Scopus subject areas
- Medicine(all)
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Capillary electrophoresis of peptides and proteins using isoelectric buffers. / Righetti, P. G.; Bossi, A.; Gelfi, C.
In: Current Protocols in Protein Science, Vol. Chapter 10, 05.2001.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Capillary electrophoresis of peptides and proteins using isoelectric buffers.
AU - Righetti, P. G.
AU - Bossi, A.
AU - Gelfi, C.
PY - 2001/5
Y1 - 2001/5
N2 - Capillary electrophoresis in acidic isoelectric buffers is a novel methodology allowing fast protein and peptide analysis in uncoated capillaries. Due to the low pH adopted and to the use of dynamic coating with cellulose derivatives, silanol ionization is essentially suppressed and no interaction of macromolecules with the untreated wall ensues. In addition, because of the low conductivity of quasi-stationary isoelectric buffers, high voltage gradients can be applied (up to 800 V/cm), permitting fast peptide analysis with a high resolving power and minimal diffusional peak spreading.
AB - Capillary electrophoresis in acidic isoelectric buffers is a novel methodology allowing fast protein and peptide analysis in uncoated capillaries. Due to the low pH adopted and to the use of dynamic coating with cellulose derivatives, silanol ionization is essentially suppressed and no interaction of macromolecules with the untreated wall ensues. In addition, because of the low conductivity of quasi-stationary isoelectric buffers, high voltage gradients can be applied (up to 800 V/cm), permitting fast peptide analysis with a high resolving power and minimal diffusional peak spreading.
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UR - http://www.scopus.com/inward/citedby.url?scp=43049183480&partnerID=8YFLogxK
M3 - Article
C2 - 18429094
AN - SCOPUS:43049183480
VL - Chapter 10
JO - Current Protocols in Protein Science
JF - Current Protocols in Protein Science
SN - 1934-3655
ER -