Abstract
The use of capillary zone electrophoresis (CZE) in polymer networks for the analysis of an 8 bp (base pair) deletion in congenital adrenal hyperplasia was investigated. Separations were performed in Tris-borate-EDTA buffer (pH 8.3) containing 6% liquid linear polyacrylamide as a sieving dynamic matrix and 10 μM ethidium bromide for improving DNA fragment separation. Easy analysis and detection of the 127 and 135 bp amplified fragments was accomplished. The capillary column can be used for >50 analyses before degradation and loss of resolution. The results are compatible to those obtained by gel-slab zone electrophoresis in a 12%T, 4%C polyacrylamide matrix. The sensitivity, by simple UV absorption at 254 nm, is similar to that obtained in gel slabs by dye intercalation staining.
| Original language | English |
|---|---|
| Pages (from-to) | 201-205 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 657 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 1 1994 |
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ASJC Scopus subject areas
- Chemistry(all)
Cite this
Capillary zone electrophoresis in polymer networks of polymerase chain reaction-amplified oligonucleotides : the case of congenital adrenal hyperplasia. / Gelfi, Cecilia; Orsi, Antonia; Righetti, Pier Giorgio; Zanussi, Monica; Carrera, Paola; Ferrari, Maurizio.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 657, No. 1, 01.07.1994, p. 201-205.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Capillary zone electrophoresis in polymer networks of polymerase chain reaction-amplified oligonucleotides
T2 - the case of congenital adrenal hyperplasia
AU - Gelfi, Cecilia
AU - Orsi, Antonia
AU - Righetti, Pier Giorgio
AU - Zanussi, Monica
AU - Carrera, Paola
AU - Ferrari, Maurizio
PY - 1994/7/1
Y1 - 1994/7/1
N2 - The use of capillary zone electrophoresis (CZE) in polymer networks for the analysis of an 8 bp (base pair) deletion in congenital adrenal hyperplasia was investigated. Separations were performed in Tris-borate-EDTA buffer (pH 8.3) containing 6% liquid linear polyacrylamide as a sieving dynamic matrix and 10 μM ethidium bromide for improving DNA fragment separation. Easy analysis and detection of the 127 and 135 bp amplified fragments was accomplished. The capillary column can be used for >50 analyses before degradation and loss of resolution. The results are compatible to those obtained by gel-slab zone electrophoresis in a 12%T, 4%C polyacrylamide matrix. The sensitivity, by simple UV absorption at 254 nm, is similar to that obtained in gel slabs by dye intercalation staining.
AB - The use of capillary zone electrophoresis (CZE) in polymer networks for the analysis of an 8 bp (base pair) deletion in congenital adrenal hyperplasia was investigated. Separations were performed in Tris-borate-EDTA buffer (pH 8.3) containing 6% liquid linear polyacrylamide as a sieving dynamic matrix and 10 μM ethidium bromide for improving DNA fragment separation. Easy analysis and detection of the 127 and 135 bp amplified fragments was accomplished. The capillary column can be used for >50 analyses before degradation and loss of resolution. The results are compatible to those obtained by gel-slab zone electrophoresis in a 12%T, 4%C polyacrylamide matrix. The sensitivity, by simple UV absorption at 254 nm, is similar to that obtained in gel slabs by dye intercalation staining.
UR - http://www.scopus.com/inward/record.url?scp=0028216412&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028216412&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(94)80088-X
DO - 10.1016/0378-4347(94)80088-X
M3 - Article
C2 - 7952069
AN - SCOPUS:0028216412
VL - 657
SP - 201
EP - 205
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 1
ER -