Caprine herpesvirus 1 (CpHV-1) as a potential candidate for oncolytic virotherapy

Serena Montagnaro, Sara Damiano, Roberto Ciarcia, Maria Valeria Puzio, Gianmarco Ferrara, Valentina Iovane, Iris Maria Forte, Antonio Giordano, Ugo Pagnini

Research output: Contribution to journalArticle

Abstract

Caprine Herpesvirus type 1 (CpHV-1) is a species-specific herpes virus able to induce apoptosis in several biological systems. In the present study we aimed to investigate the ability of CpHV-1 to reduce cells viability, to replicate and to cause cell death also in human cancer cell lines. We tested the CpHV-1 effects on HEL-299, Vero, MDA-MB-468, HeLa, U2OS, PC3, A549 and K562 neoplastic cell lines and on MDBK cells. Firstly, we evaluated the effect of CpHV-1 infection on cell viability by MTT assay and our data showed that CpHV-1 can induce a marked cytopathic effect (CPE) in most of cell lines tested, except for HEL-299, Vero and K562 cells. The reduction of cell viability was associated with a significant increase of viral production. We next investigated if CpHV-1 was able to induce cell death and so through western blotting analysis we evaluated cleaved caspase 3, LC3II and p62 protein levels after infection. Caspase 3 activation was detected in MDBK cells and, even if at different times p.i., also in MDA-MB-468, U2OS, and PC3 cell lines, while LC3II increase and concomitant p62 protein reduction were observed only in U2OS, and A549 cells, no significant alteration of these proteins was observed in the other cell lines tested. Finally, to confirm virus ability to trigger apoptosis we performed an Annexin-V apoptosis test after 24 h p.i. Although we need to further explore mechanisms underlying CpHV-1 treatment, this study could serve as the basis for the development of new treatment options aiming to fight several cancer types.

Original languageEnglish
JournalCancer Biology and Therapy
DOIs
Publication statusPublished - 2019

Fingerprint

Varicellovirus
Oncolytic Virotherapy
Cell Line
Cell Survival
K562 Cells
Apoptosis
Caspase 3
Cell Death
Viruses
Herpesviridae Infections
Proteins
Vero Cells
Annexin A5
Cause of Death
Neoplasms
Western Blotting

Keywords

  • apoptosis
  • autophagy
  • human cancer cells
  • Oncolytic virotherapy

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research

Cite this

Montagnaro, S., Damiano, S., Ciarcia, R., Puzio, M. V., Ferrara, G., Iovane, V., ... Pagnini, U. (2019). Caprine herpesvirus 1 (CpHV-1) as a potential candidate for oncolytic virotherapy. Cancer Biology and Therapy. https://doi.org/10.1080/15384047.2018.1504722

Caprine herpesvirus 1 (CpHV-1) as a potential candidate for oncolytic virotherapy. / Montagnaro, Serena; Damiano, Sara; Ciarcia, Roberto; Puzio, Maria Valeria; Ferrara, Gianmarco; Iovane, Valentina; Forte, Iris Maria; Giordano, Antonio; Pagnini, Ugo.

In: Cancer Biology and Therapy, 2019.

Research output: Contribution to journalArticle

Montagnaro, S, Damiano, S, Ciarcia, R, Puzio, MV, Ferrara, G, Iovane, V, Forte, IM, Giordano, A & Pagnini, U 2019, 'Caprine herpesvirus 1 (CpHV-1) as a potential candidate for oncolytic virotherapy' Cancer Biology and Therapy. https://doi.org/10.1080/15384047.2018.1504722
Montagnaro, Serena ; Damiano, Sara ; Ciarcia, Roberto ; Puzio, Maria Valeria ; Ferrara, Gianmarco ; Iovane, Valentina ; Forte, Iris Maria ; Giordano, Antonio ; Pagnini, Ugo. / Caprine herpesvirus 1 (CpHV-1) as a potential candidate for oncolytic virotherapy. In: Cancer Biology and Therapy. 2019.
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