Carbohydrate-binding agents (CBAs) inhibit HIV-1 infection in human primary monocyte-derived macrophages (MDMs) and efficiently prevent MDM-directed viral capture and subsequent transmission to CD4+ T lymphocytes

M. Pollicita, D. Schols, S. Aquaro, W. J. Peumans, E. J M Van Damme, C. F. Perno, J. Balzarini

Research output: Contribution to journalArticle

Abstract

Carbohydrate-binding agents (CBAs) have been proposed as innovative anti-HIV compounds selectively targeting the glycans of the HIV-1 envelope glycoprotein gp120 and preventing DC-SIGN-directed HIV capture by dendritic cells (DCs) and transmission to CD4+ T-lymphocytes. We now show that CBAs efficiently prevent R5 HIV-1 infection of human primary monocyte-derived macrophage (MDM) cell cultures in the nanomolar range. Both R5 and X4 HIV-1 strains were efficiently captured by the macrophage mannose-binding receptor (MMR) present on MDM. HIV-1 capture by MMR-expressing MDM was inhibited by soluble mannose-binding lectin and MMR antibody. Short pre-exposure of these HIV-1 strains to CBAs is able to prevent virus capture by MDM and subsequent syncytia formation in cocultures of the CBA-exposed HIV-1-captured MDM and uninfected CD4+ T-lymphocytes. The potential of CBAs to impair MDM in their capacity to capture and to transmit HIV to T-lymphocytes might be an important property to be taken into consideration in the eventual choice to select microbicide candidate drugs for clinical investigation.

Original languageEnglish
Pages (from-to)382-391
Number of pages10
JournalVirology
Volume370
Issue number2
DOIs
Publication statusPublished - Jan 20 2008

Keywords

  • Carbohydrate-binding agents (CBAs)
  • DC-SIGN
  • Envelope glycoprotein gp120
  • Glycans
  • HIV-1
  • HIV-1 capture
  • HIV-1 transmission
  • Macrophage mannose-binding receptor (MMR)
  • Monocyte-derived macrophages (MDMs)

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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