Carbon monoxide relaxes the female pig urethra as effectively as nitric oxide in the presence of YC-1

Annette Schröder, Petter Hedlund, Karl Erik Andersson

Research output: Contribution to journalArticle

Abstract

Purpose: Nitric oxide (NO) and carbon monoxide (CO) have been suggested to relax smooth muscle by activating soluble guanylate cyclase (sGC), binding to the same site of the enzyme. 3-(5′-hydroxymethyl-2′-furyl)-l-benzylindazole (YC-1) (Cayman Co., Malmö, Sweden) increases the catalytic rate of sGC by binding to an allosteric site. We investigated whether YC-1 can modulate the relaxant responses of isolated urethral smooth muscle to exogenous CO, (NO) and electrical field stimulation. Materials and Methods: In spontaneously active and noradrenaline (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) pre-contracted preparations of circular urethral smooth muscle from female pigs relaxant responses were evoked by electrical field stimulation before and after incubation with 10-5 M. YC-1. The concentration-response curves for CO and NO were investigated in noradrenaline pre-contracted strips before and after incubation with YC-1. The tissue contents of cyclic 3′,5′-guanosine monophosphate (cGMP) and cyclic adenosine monophosphate after electrical field stimulation, and the administration of CO or NO was investigated in the absence and presence of YC-1. Results: YC-1 significantly increased the amplitude of the relaxations evoked by electrical field stimulation, CO and NO, and simultaneously caused significant increases in the cGMP content in all preparations. The effect on CO induced relaxant responses was conspicuous. In the presence of YC-1 the potency and maximal relaxant effect of CO were similar to those of NO in the absence of YC-1. Conclusions: YC-1 enhances cGMP dependent relaxant responses of the female pig urethra in vitro. The finding that the response to CO was greatly increased after sensitizing sGC suggests a potential for CO as a relaxant mediator in urethral smooth muscle.

Original languageEnglish
Pages (from-to)1892-1896
Number of pages5
JournalJournal of Urology
Volume167
Issue number4 I
Publication statusPublished - 2002

Keywords

  • Cyclic GMP
  • Guanylate cyclase
  • Muscle relaxation
  • Muscle, smooth
  • Urethra

ASJC Scopus subject areas

  • Urology

Fingerprint Dive into the research topics of 'Carbon monoxide relaxes the female pig urethra as effectively as nitric oxide in the presence of YC-1'. Together they form a unique fingerprint.

  • Cite this

    Schröder, A., Hedlund, P., & Andersson, K. E. (2002). Carbon monoxide relaxes the female pig urethra as effectively as nitric oxide in the presence of YC-1. Journal of Urology, 167(4 I), 1892-1896.