Carboxyfullerenes protect human keratinocytes from ultraviolet-B-induced apoptosis

Cristiana Fumelli, Alessandra Marconi, Stefano Salvioli, Elisabetta Straface, Walter Malorni, Anna Maria Offidani, Roberto Pellicciari, Gennaro Schettini, Alberto Giannetti, Daniela Monti, Claudio Franceschi, Carlo Pincelli

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Carboxyfullerene, a water-soluble carboxylic acid derivative of a fullerene, which acts as a free-radical scavenger, was investigated as a protective agent against ultraviolet-light-induced damage in human keratinocytes. First, we demonstrate that carboxyfullerene is not cytotoxic for these cells. In addition, this compound significantly reduces the ultraviolet-B-induced inhibition of keratinocyte proliferation and protects keratinocytes from apoptosis caused by ultraviolet B irradiation in a time- and dose-dependent fashion. Furthermore, the percentage of cells with depolarized mitochondria is significantly lower in ultraviolet-B-irradiated keratinocytes pretreated with carboxyfullerene than in cells provided with diluent alone. Carboxyfullerene also protects human keratinocytes from apoptosis induced by exposure to deoxy-D-ribose, a sugar that causes cell death through a pathway involving oxidative stress. On the other hand, ultraviolet B downregulates bcl-2 levels in human keratinocytes, and carboxyfullerene fails to prevent this effect. These results suggest that carboxyfullerene protects human keratinocytes from ultraviolet B damage possibly via a mechanism interfering with the generation of reactive oxygen species from depolarized mitochondria without the involvement of bcl-2.

Original languageEnglish
Pages (from-to)835-841
Number of pages7
JournalJournal of Investigative Dermatology
Volume115
Issue number5
DOIs
Publication statusPublished - 2000

Fingerprint

Keratinocytes
Apoptosis
Mitochondria
Fullerenes
Protective Agents
Free Radical Scavengers
Oxidative stress
Ribose
Cell death
Ultraviolet Rays
Carboxylic Acids
Sugars
Dosimetry
Cause of Death
Reactive Oxygen Species
Oxidative Stress
Cell Death
Down-Regulation
Irradiation
Derivatives

Keywords

  • Apoptosis
  • Carboxyfullerenes
  • Keratinocytes
  • Ultraviolet radiation

ASJC Scopus subject areas

  • Dermatology

Cite this

Fumelli, C., Marconi, A., Salvioli, S., Straface, E., Malorni, W., Offidani, A. M., ... Pincelli, C. (2000). Carboxyfullerenes protect human keratinocytes from ultraviolet-B-induced apoptosis. Journal of Investigative Dermatology, 115(5), 835-841. https://doi.org/10.1046/j.1523-1747.2000.00140.x

Carboxyfullerenes protect human keratinocytes from ultraviolet-B-induced apoptosis. / Fumelli, Cristiana; Marconi, Alessandra; Salvioli, Stefano; Straface, Elisabetta; Malorni, Walter; Offidani, Anna Maria; Pellicciari, Roberto; Schettini, Gennaro; Giannetti, Alberto; Monti, Daniela; Franceschi, Claudio; Pincelli, Carlo.

In: Journal of Investigative Dermatology, Vol. 115, No. 5, 2000, p. 835-841.

Research output: Contribution to journalArticle

Fumelli, C, Marconi, A, Salvioli, S, Straface, E, Malorni, W, Offidani, AM, Pellicciari, R, Schettini, G, Giannetti, A, Monti, D, Franceschi, C & Pincelli, C 2000, 'Carboxyfullerenes protect human keratinocytes from ultraviolet-B-induced apoptosis', Journal of Investigative Dermatology, vol. 115, no. 5, pp. 835-841. https://doi.org/10.1046/j.1523-1747.2000.00140.x
Fumelli, Cristiana ; Marconi, Alessandra ; Salvioli, Stefano ; Straface, Elisabetta ; Malorni, Walter ; Offidani, Anna Maria ; Pellicciari, Roberto ; Schettini, Gennaro ; Giannetti, Alberto ; Monti, Daniela ; Franceschi, Claudio ; Pincelli, Carlo. / Carboxyfullerenes protect human keratinocytes from ultraviolet-B-induced apoptosis. In: Journal of Investigative Dermatology. 2000 ; Vol. 115, No. 5. pp. 835-841.
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