Cartilage graft engineering by co-culturing primary human articular chondrocytes with human bone marrow stromal cells

Maria Antonietta Sabatino, Rosaria Santoro, Sinan Gueven, Claude Jaquiery, David James Wendt, Ivan Martin, Matteo Moretti, Andrea Barbero

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Co-culture of mesenchymal stromal cells (MSCs) with articular chondrocytes (ACs) has been reported to improve the efficiency of utilization of a small number of ACs for the engineering of implantable cartilaginous tissues. However, the use of cells of animal origin and the generation of small-scale micromass tissues limit the clinical relevance of previous studies. Here we investigated the in vitro and in vivo chondrogenic capacities of scaffold-based constructs generated by combining primary human ACs with human bone marrow MSCs (BM-MSCs). The two cell types were cultured in collagen sponges (2 × 6 mm disks) at the BM-MSCs:ACs ratios: 100:0, 95:5, 75:25 and 0:100 for 3 weeks. Scaffolds freshly seeded or further precultured in vitro for 2 weeks were also implanted subcutaneously in nude mice and harvested after 8 or 6 weeks, respectively. Static co-culture of ACs (25%) with BM-MSCs (75%) in scaffolds resulted in up to 1.4-fold higher glycosaminoglycan (GAG) content than what would be expected based on the relative percentages of the different cell types. In vivo GAG induction was drastically enhanced by the in vitro preculture and maximal at the ratio 95:5 (3.8-fold higher). Immunostaining analyses revealed enhanced accumulation of type II collagen and reduced accumulation of type X collagen with increasing ACs percentage. Constructs generated in the perfusion bioreactor system were homogeneously cellularized. In summary, human cartilage grafts were successfully generated, culturing BM-MSCs with a relatively low fraction of non-expanded ACs in porous scaffolds. The proposed co-culture strategy is directly relevant towards a single-stage surgical procedure for cartilage repair.

Original languageEnglish
Pages (from-to)1394-1403
Number of pages10
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

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Cartilage
Chondrocytes
Mesenchymal Stromal Cells
Grafts
Scaffolds
Bone
Joints
Collagen
Transplants
Glycosaminoglycans
Coculture Techniques
Collagen Type X
Tissue
Bone Marrow
Collagen Type II
Bioreactors
Animals
Repair
Porifera
Nude Mice

Keywords

  • Bioreactor culture
  • Chondrocytes, cartilage engineering
  • Mesenchymal stem cells
  • Nude mice

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Cartilage graft engineering by co-culturing primary human articular chondrocytes with human bone marrow stromal cells. / Sabatino, Maria Antonietta; Santoro, Rosaria; Gueven, Sinan; Jaquiery, Claude; Wendt, David James; Martin, Ivan; Moretti, Matteo; Barbero, Andrea.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 9, No. 12, 01.12.2015, p. 1394-1403.

Research output: Contribution to journalArticle

Sabatino, Maria Antonietta ; Santoro, Rosaria ; Gueven, Sinan ; Jaquiery, Claude ; Wendt, David James ; Martin, Ivan ; Moretti, Matteo ; Barbero, Andrea. / Cartilage graft engineering by co-culturing primary human articular chondrocytes with human bone marrow stromal cells. In: Journal of Tissue Engineering and Regenerative Medicine. 2015 ; Vol. 9, No. 12. pp. 1394-1403.
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