Abstract
The aim of this study was to investigate the possibility of using the benzyl ester of hyaluronic acid (HYAFF® 11), a recently developed semisynthetic resorbable material, as a scaffold for the culture of human nasoseptal chondrocytes in tissue-engineering procedures of cartilage reconstruction. Different techniques such as immunohistochemistry, scanning electron microscopy, and confocal laser scanning microscopy were used to study the behavior, morphology, and phenotype expression of the chondrocytes, which were initially expanded and then seeded on the material. The nonwoven cell carrier allowed good viability and adhesivity of the cells without any surface treatment with additional substances. Furthermore, the cultured cells expressed cartilage-specific collagen type II, indicating that they were able to redifferentiate within the scaffold of HYAFF® 11 and were able to retain a chondrocyte phenotype even after a long period of in vitro conditions. Nevertheless, the expression of collagen type I, which was produced by dedifferentiated or incompletely redifferentiated chondrocytes, was noticeable. Additional data were obtained by subcutaneous implantation of samples seeded with human cells in the in vivo model of the athymic nude mouse. The results after 1 month revealed the development of tissue similar to hyaline cartilage. This study is promising for the use of this scaffold for tissue engineering of cartilage replacements.
| Original language | English |
|---|---|
| Pages (from-to) | 172-181 |
| Number of pages | 10 |
| Journal | Journal of Biomedical Materials Research |
| Volume | 42 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Nov 1998 |
Fingerprint
Keywords
- Bioresorbable materials
- Cartilage
- Confocal laser scanning microscopy
- Hyaluronic acid
- Tissue engineering
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
Cite this
Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester. / Aigner, J.; Tegeler, J.; Hutzler, P.; Campoccia, D.; Pavesio, A.; Hammer, C.; Kastenbauer, E.; Naumann, A.
In: Journal of Biomedical Materials Research, Vol. 42, No. 2, 11.1998, p. 172-181.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester
AU - Aigner, J.
AU - Tegeler, J.
AU - Hutzler, P.
AU - Campoccia, D.
AU - Pavesio, A.
AU - Hammer, C.
AU - Kastenbauer, E.
AU - Naumann, A.
PY - 1998/11
Y1 - 1998/11
N2 - The aim of this study was to investigate the possibility of using the benzyl ester of hyaluronic acid (HYAFF® 11), a recently developed semisynthetic resorbable material, as a scaffold for the culture of human nasoseptal chondrocytes in tissue-engineering procedures of cartilage reconstruction. Different techniques such as immunohistochemistry, scanning electron microscopy, and confocal laser scanning microscopy were used to study the behavior, morphology, and phenotype expression of the chondrocytes, which were initially expanded and then seeded on the material. The nonwoven cell carrier allowed good viability and adhesivity of the cells without any surface treatment with additional substances. Furthermore, the cultured cells expressed cartilage-specific collagen type II, indicating that they were able to redifferentiate within the scaffold of HYAFF® 11 and were able to retain a chondrocyte phenotype even after a long period of in vitro conditions. Nevertheless, the expression of collagen type I, which was produced by dedifferentiated or incompletely redifferentiated chondrocytes, was noticeable. Additional data were obtained by subcutaneous implantation of samples seeded with human cells in the in vivo model of the athymic nude mouse. The results after 1 month revealed the development of tissue similar to hyaline cartilage. This study is promising for the use of this scaffold for tissue engineering of cartilage replacements.
AB - The aim of this study was to investigate the possibility of using the benzyl ester of hyaluronic acid (HYAFF® 11), a recently developed semisynthetic resorbable material, as a scaffold for the culture of human nasoseptal chondrocytes in tissue-engineering procedures of cartilage reconstruction. Different techniques such as immunohistochemistry, scanning electron microscopy, and confocal laser scanning microscopy were used to study the behavior, morphology, and phenotype expression of the chondrocytes, which were initially expanded and then seeded on the material. The nonwoven cell carrier allowed good viability and adhesivity of the cells without any surface treatment with additional substances. Furthermore, the cultured cells expressed cartilage-specific collagen type II, indicating that they were able to redifferentiate within the scaffold of HYAFF® 11 and were able to retain a chondrocyte phenotype even after a long period of in vitro conditions. Nevertheless, the expression of collagen type I, which was produced by dedifferentiated or incompletely redifferentiated chondrocytes, was noticeable. Additional data were obtained by subcutaneous implantation of samples seeded with human cells in the in vivo model of the athymic nude mouse. The results after 1 month revealed the development of tissue similar to hyaline cartilage. This study is promising for the use of this scaffold for tissue engineering of cartilage replacements.
KW - Bioresorbable materials
KW - Cartilage
KW - Confocal laser scanning microscopy
KW - Hyaluronic acid
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=0032210909&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032210909&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4636(199811)42:2<172::AID-JBM2>3.0.CO;2-M
DO - 10.1002/(SICI)1097-4636(199811)42:2<172::AID-JBM2>3.0.CO;2-M
M3 - Article
C2 - 9773813
AN - SCOPUS:0032210909
VL - 42
SP - 172
EP - 181
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 2
ER -