Caspase-1 levels in biological fluids from patients with multiple sclerosis and from patients with other neurological and non-neurological diseases

Diego Franciotta, Gianvito Martino, Elisabetta Zardini, Roberto Furlan, Roberto Bergamaschi, Maira Gironi, Alessandra Bergami, Giovanna Angelini, Fabrizio De Benedetti, Patrizia Pignatti, Gianna Moscato, Vittorio Cosi

Research output: Contribution to journalArticle

Abstract

In multiple sclerosis (MS), pathological white matter damage in the central nervous system is sustained by immune-inflammatory response. Caspase-1 plays a pivotal role in immune-mediated inflammation, as it regulates the cellular export of IL-1β and IL-18. We carried out a preliminary in vitro study of the kinetics of extracellular caspase-1 release. We then measured caspase-1 levels in paired serum and cerebrospinal fluid (CSF) samples of 75 MS patients, 15 healthy subjects, and patients with other neurological diseases. Paired synovial fluid and serum samples of patients with juvenile idiopathic arthritis, and paired sputum and serum samples of asthma patients were also studied. Mean serum caspase-1 concentrations did not differ between groups. Caspase-1 was detected in the CSF of patients with acute, but not stable, MS [7.5 ± (SEM) 0.9 pg/ml; test's sensitivity, 56% and specificity, 100%]. Its levels correlated with pleocytosis. The highest mean caspase-1 levels were found in the arthritic synovial fluids (945.5 ± 126.6 pg/ml, which correlated with erythrocyte sedimentation rate), and in the sputum samples (370.1 ± 71.0 pg/ml, which correlated with the number of macrophages in the sputum). On condition that caspase-1 is determined in the fluids pertaining to the disease-specific inflammatory sites, its level is a reliable marker of ongoing immune-inflammatory response. The enzyme measurement in CSF can also help define state-trait in MS.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalEuropean Cytokine Network
Volume13
Issue number1
Publication statusPublished - 2002

Keywords

  • Asthma
  • Cerebrospinal fluid
  • Juvenile idiopathic arthritis
  • Sputum
  • Synovial fluid
  • THP-1 cells

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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