Caspase 3 activation and PARP cleavage in lymphocytes from newborn babies of diabetic mothers with unbalanced glycaemic control

F. Tarquini, R. Tiribuzi, L. Crispoltoni, S. Porcellati, A. M. Del Pino, A. Orlacchio, G. Coata, S. Arnone, E. Torlone, B. Cappuccini, G. C. Di Renzo, A. Orlacchio

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Several epidemiological studies showed that gestational diabetes mellitus is the most frequent metabolic disorder of pregnancy, the pathogenesis of which has yet to be completely clarified. The aim of this study was to investigate the presence and processing of caspase 3 (Casp3) and poly(ADP-ribose) polymerase 1 (PARP1) in cord blood lymphocytes as markers of apoptosis in relation to glycaemic control during intrauterine life. Our results showed a specific positive correlation between the levels of active Casp3 (17-19kDa) and the inactive form of PARP1 (89kDa) in lymphocytes isolated from newborn babies of diabetic women with unbalanced glycaemic control, with a direct correlation between the activation of casp3 and the inactivation of PARP1, that makes lymphocytes unresponsive towards lipopolysaccharide stimulation, highlighting an altered functional response. Besides more studies are required to fully correlate the activation of the apoptotic process during the intrauterine life with the foetal health later in life, our study indicates that a cord blood lymphocyte, an easily accessible source, is informative about the activation of apoptotic stimuli in circulating cells of newborn babies in relation to the glycaemic control reached by the mother during pregnancy.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalCell Biochemistry and Function
Volume32
Issue number1
DOIs
Publication statusPublished - Jan 2014

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Lymphocytes
Caspase 3
Poly(ADP-ribose) Polymerases
Chemical activation
Mothers
Newborn Infant
Fetal Blood
Blood
Pregnancy
Gestational Diabetes
Medical problems
Lipopolysaccharides
Epidemiologic Studies
Health
Apoptosis
Processing
Poly (ADP-Ribose) Polymerase-1

Keywords

  • Caspase 3
  • Gestational diabetes mellitus
  • Lymphocytes
  • PARP

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

Caspase 3 activation and PARP cleavage in lymphocytes from newborn babies of diabetic mothers with unbalanced glycaemic control. / Tarquini, F.; Tiribuzi, R.; Crispoltoni, L.; Porcellati, S.; Del Pino, A. M.; Orlacchio, A.; Coata, G.; Arnone, S.; Torlone, E.; Cappuccini, B.; Di Renzo, G. C.; Orlacchio, A.

In: Cell Biochemistry and Function, Vol. 32, No. 1, 01.2014, p. 87-95.

Research output: Contribution to journalArticle

Tarquini, F, Tiribuzi, R, Crispoltoni, L, Porcellati, S, Del Pino, AM, Orlacchio, A, Coata, G, Arnone, S, Torlone, E, Cappuccini, B, Di Renzo, GC & Orlacchio, A 2014, 'Caspase 3 activation and PARP cleavage in lymphocytes from newborn babies of diabetic mothers with unbalanced glycaemic control', Cell Biochemistry and Function, vol. 32, no. 1, pp. 87-95. https://doi.org/10.1002/cbf.2975
Tarquini, F. ; Tiribuzi, R. ; Crispoltoni, L. ; Porcellati, S. ; Del Pino, A. M. ; Orlacchio, A. ; Coata, G. ; Arnone, S. ; Torlone, E. ; Cappuccini, B. ; Di Renzo, G. C. ; Orlacchio, A. / Caspase 3 activation and PARP cleavage in lymphocytes from newborn babies of diabetic mothers with unbalanced glycaemic control. In: Cell Biochemistry and Function. 2014 ; Vol. 32, No. 1. pp. 87-95.
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