Ca2+ transport and permeability in inside-out red cell membrane vesicles after freezing

A. Rubinacci; B. Fuller; F. Wuytack; W. De Loecker

doi:10.1016/0011-2240(86)90004-0

Ca²⁺ transport and permeability in inside-out red cell membrane vesicles after freezing

A. Rubinacci, B. Fuller, F. Wuytack, W. De Loecker

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

To evaluate the effects of freezing and thawing on Ca²⁺ transport and permeability, inside-out red cell membrane vesicles (IORCMV) are examined. Exposure to the cryoprotectant Me₂SO as well as different cooling regimes on unprotected and cryoprotected vesicles do not affect the membrane Ca²⁺ transport. However, freezing and thawing increase the membrane permeability to sucrose.

Original language	English
Pages (from-to)	134-140
Number of pages	7
Journal	Cryobiology
Volume	23
Issue number	2
DOIs	https://doi.org/10.1016/0011-2240(86)90004-0
Publication status	Published - 1986

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)

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title = "Ca2+ transport and permeability in inside-out red cell membrane vesicles after freezing",

abstract = "To evaluate the effects of freezing and thawing on Ca2+ transport and permeability, inside-out red cell membrane vesicles (IORCMV) are examined. Exposure to the cryoprotectant Me2SO as well as different cooling regimes on unprotected and cryoprotected vesicles do not affect the membrane Ca2+ transport. However, freezing and thawing increase the membrane permeability to sucrose.",

author = "A. Rubinacci and B. Fuller and F. Wuytack and {De Loecker}, W.",

year = "1986",

doi = "10.1016/0011-2240(86)90004-0",

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T1 - Ca2+ transport and permeability in inside-out red cell membrane vesicles after freezing

AU - Rubinacci, A.

AU - Fuller, B.

AU - Wuytack, F.

AU - De Loecker, W.

PY - 1986

Y1 - 1986

N2 - To evaluate the effects of freezing and thawing on Ca2+ transport and permeability, inside-out red cell membrane vesicles (IORCMV) are examined. Exposure to the cryoprotectant Me2SO as well as different cooling regimes on unprotected and cryoprotected vesicles do not affect the membrane Ca2+ transport. However, freezing and thawing increase the membrane permeability to sucrose.

AB - To evaluate the effects of freezing and thawing on Ca2+ transport and permeability, inside-out red cell membrane vesicles (IORCMV) are examined. Exposure to the cryoprotectant Me2SO as well as different cooling regimes on unprotected and cryoprotected vesicles do not affect the membrane Ca2+ transport. However, freezing and thawing increase the membrane permeability to sucrose.

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