Ca2+ transport and permeability in inside-out red cell membrane vesicles after freezing

A. Rubinacci; B. Fuller; F. Wuytack; W. De Loecker

doi:10.1016/0011-2240(86)90004-0

Ca²⁺ transport and permeability in inside-out red cell membrane vesicles after freezing

A. Rubinacci, B. Fuller, F. Wuytack, W. De Loecker

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

To evaluate the effects of freezing and thawing on Ca²⁺ transport and permeability, inside-out red cell membrane vesicles (IORCMV) are examined. Exposure to the cryoprotectant Me₂SO as well as different cooling regimes on unprotected and cryoprotected vesicles do not affect the membrane Ca²⁺ transport. However, freezing and thawing increase the membrane permeability to sucrose.

Original language	English
Pages (from-to)	134-140
Number of pages	7
Journal	Cryobiology
Volume	23
Issue number	2
DOIs	https://doi.org/10.1016/0011-2240(86)90004-0
Publication status	Published - 1986

Fingerprint

Thawing

Cell membranes

Freezing

thawing

cell membranes

Permeability

permeability

freezing

Cell Membrane

Membranes

calcium

membrane permeability

cryoprotectants

Sucrose

cooling

sucrose

Cooling

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)

Cite this

Rubinacci, A., Fuller, B., Wuytack, F., & De Loecker, W. (1986). Ca²⁺ transport and permeability in inside-out red cell membrane vesicles after freezing. Cryobiology, 23(2), 134-140. https://doi.org/10.1016/0011-2240(86)90004-0

Ca²⁺ transport and permeability in inside-out red cell membrane vesicles after freezing. / Rubinacci, A.; Fuller, B.; Wuytack, F.; De Loecker, W.

In: Cryobiology, Vol. 23, No. 2, 1986, p. 134-140.

Research output: Contribution to journal › Article

Rubinacci, A, Fuller, B, Wuytack, F & De Loecker, W 1986, 'Ca²⁺ transport and permeability in inside-out red cell membrane vesicles after freezing', Cryobiology, vol. 23, no. 2, pp. 134-140. https://doi.org/10.1016/0011-2240(86)90004-0

Rubinacci A, Fuller B, Wuytack F, De Loecker W. Ca²⁺ transport and permeability in inside-out red cell membrane vesicles after freezing. Cryobiology. 1986;23(2):134-140. https://doi.org/10.1016/0011-2240(86)90004-0

Rubinacci, A. ; Fuller, B. ; Wuytack, F. ; De Loecker, W. / Ca²⁺ transport and permeability in inside-out red cell membrane vesicles after freezing. In: Cryobiology. 1986 ; Vol. 23, No. 2. pp. 134-140.

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10.1016/0011-2240(86)90004-0