Abstract
A large (65%) fraction of in vitro cultured rat chromaffin cells exhibit spontaneous [Ca2+]i oscillations, and the rest can be recruited to oscillate by appropriate stimulations. Based on fura-2 single cell [ca2+]i measurements, evidence is provided that these oscillation originate, via the activation of Ca2+-induced Ca2+-release, from intracellular Ca2+ stores in rapid equilibrium with extracellular Ca2+. By combining [Ca2+]i measurements with a specific plaque secretion assay we demonstrate that oscillating cells exhibit a spontaneous exocytic secretory activity whereas the cells with stable [Ca2+]i do not, [Ca2+]i oscillations appear therefore to account for the high unstimulated catecholamine release, an activity typical of the chromaffin cells of the rat.
Original language | English |
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Pages (from-to) | 169-172 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 283 |
Issue number | 2 |
DOIs | |
Publication status | Published - Jun 3 1991 |
Keywords
- Ca
- Ca-induced Ca-release
- Chromaffin cell
- Exocytosis
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
- Cell Biology
- Genetics
- Structural Biology