[Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat

Antonio Malgaroli, Jacopo Meldolesi

Research output: Contribution to journal › Article

Abstract

A large (65%) fraction of in vitro cultured rat chromaffin cells exhibit spontaneous [Ca2+]i oscillations, and the rest can be recruited to oscillate by appropriate stimulations. Based on fura-2 single cell [ca2+]i measurements, evidence is provided that these oscillation originate, via the activation of Ca2+-induced Ca2+-release, from intracellular Ca2+ stores in rapid equilibrium with extracellular Ca2+. By combining [Ca2+]i measurements with a specific plaque secretion assay we demonstrate that oscillating cells exhibit a spontaneous exocytic secretory activity whereas the cells with stable [Ca2+]i do not, [Ca2+]i oscillations appear therefore to account for the high unstimulated catecholamine release, an activity typical of the chromaffin cells of the rat.

Original languageEnglish
Pages (from-to)169-172
Number of pages4
JournalFEBS Letters
Volume283
Issue number2
DOIs
Publication statusPublished - Jun 3 1991

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Chromaffin Cells
Rats
Cells
Fura-2
Catecholamines
Assays
Chemical activation

Keywords

  • Ca
  • Ca-induced Ca-release
  • Chromaffin cell
  • Exocytosis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

Cite this

[Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat. / Malgaroli, Antonio; Meldolesi, Jacopo.

In: FEBS Letters, Vol. 283, No. 2, 03.06.1991, p. 169-172.

Research output: Contribution to journal › Article

Malgaroli, A & Meldolesi, J 1991, '[Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat', FEBS Letters, vol. 283, no. 2, pp. 169-172. https://doi.org/10.1016/0014-5793(91)80580-V
Malgaroli A, Meldolesi J. [Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat. FEBS Letters. 1991 Jun 3;283(2):169-172. https://doi.org/10.1016/0014-5793(91)80580-V
Malgaroli, Antonio ; Meldolesi, Jacopo. / [Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat. In: FEBS Letters. 1991 ; Vol. 283, No. 2. pp. 169-172.
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