[Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat

Antonio Malgaroli, Jacopo Meldolesi

Research output: Contribution to journalArticle

Abstract

A large (65%) fraction of in vitro cultured rat chromaffin cells exhibit spontaneous [Ca2+]i oscillations, and the rest can be recruited to oscillate by appropriate stimulations. Based on fura-2 single cell [ca2+]i measurements, evidence is provided that these oscillation originate, via the activation of Ca2+-induced Ca2+-release, from intracellular Ca2+ stores in rapid equilibrium with extracellular Ca2+. By combining [Ca2+]i measurements with a specific plaque secretion assay we demonstrate that oscillating cells exhibit a spontaneous exocytic secretory activity whereas the cells with stable [Ca2+]i do not, [Ca2+]i oscillations appear therefore to account for the high unstimulated catecholamine release, an activity typical of the chromaffin cells of the rat.

Original languageEnglish
Pages (from-to)169-172
Number of pages4
JournalFEBS Letters
Volume283
Issue number2
DOIs
Publication statusPublished - Jun 3 1991

Fingerprint

Chromaffin Cells
Rats
Cells
Fura-2
Catecholamines
Assays
Chemical activation

Keywords

  • Ca
  • Ca-induced Ca-release
  • Chromaffin cell
  • Exocytosis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

Cite this

[Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat. / Malgaroli, Antonio; Meldolesi, Jacopo.

In: FEBS Letters, Vol. 283, No. 2, 03.06.1991, p. 169-172.

Research output: Contribution to journalArticle

@article{7f9e551a26074b34ac8b392ab455e979,
title = "[Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat",
abstract = "A large (65{\%}) fraction of in vitro cultured rat chromaffin cells exhibit spontaneous [Ca2+]i oscillations, and the rest can be recruited to oscillate by appropriate stimulations. Based on fura-2 single cell [ca2+]i measurements, evidence is provided that these oscillation originate, via the activation of Ca2+-induced Ca2+-release, from intracellular Ca2+ stores in rapid equilibrium with extracellular Ca2+. By combining [Ca2+]i measurements with a specific plaque secretion assay we demonstrate that oscillating cells exhibit a spontaneous exocytic secretory activity whereas the cells with stable [Ca2+]i do not, [Ca2+]i oscillations appear therefore to account for the high unstimulated catecholamine release, an activity typical of the chromaffin cells of the rat.",
keywords = "Ca, Ca-induced Ca-release, Chromaffin cell, Exocytosis",
author = "Antonio Malgaroli and Jacopo Meldolesi",
year = "1991",
month = "6",
day = "3",
doi = "10.1016/0014-5793(91)80580-V",
language = "English",
volume = "283",
pages = "169--172",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - [Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat

AU - Malgaroli, Antonio

AU - Meldolesi, Jacopo

PY - 1991/6/3

Y1 - 1991/6/3

N2 - A large (65%) fraction of in vitro cultured rat chromaffin cells exhibit spontaneous [Ca2+]i oscillations, and the rest can be recruited to oscillate by appropriate stimulations. Based on fura-2 single cell [ca2+]i measurements, evidence is provided that these oscillation originate, via the activation of Ca2+-induced Ca2+-release, from intracellular Ca2+ stores in rapid equilibrium with extracellular Ca2+. By combining [Ca2+]i measurements with a specific plaque secretion assay we demonstrate that oscillating cells exhibit a spontaneous exocytic secretory activity whereas the cells with stable [Ca2+]i do not, [Ca2+]i oscillations appear therefore to account for the high unstimulated catecholamine release, an activity typical of the chromaffin cells of the rat.

AB - A large (65%) fraction of in vitro cultured rat chromaffin cells exhibit spontaneous [Ca2+]i oscillations, and the rest can be recruited to oscillate by appropriate stimulations. Based on fura-2 single cell [ca2+]i measurements, evidence is provided that these oscillation originate, via the activation of Ca2+-induced Ca2+-release, from intracellular Ca2+ stores in rapid equilibrium with extracellular Ca2+. By combining [Ca2+]i measurements with a specific plaque secretion assay we demonstrate that oscillating cells exhibit a spontaneous exocytic secretory activity whereas the cells with stable [Ca2+]i do not, [Ca2+]i oscillations appear therefore to account for the high unstimulated catecholamine release, an activity typical of the chromaffin cells of the rat.

KW - Ca

KW - Ca-induced Ca-release

KW - Chromaffin cell

KW - Exocytosis

UR - http://www.scopus.com/inward/record.url?scp=0025922092&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025922092&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(91)80580-V

DO - 10.1016/0014-5793(91)80580-V

M3 - Article

C2 - 2044755

AN - SCOPUS:0025922092

VL - 283

SP - 169

EP - 172

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -