[Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat

Antonio Malgaroli, Jacopo Meldolesi

Research output: Contribution to journalArticle

Abstract

A large (65%) fraction of in vitro cultured rat chromaffin cells exhibit spontaneous [Ca2+]i oscillations, and the rest can be recruited to oscillate by appropriate stimulations. Based on fura-2 single cell [ca2+]i measurements, evidence is provided that these oscillation originate, via the activation of Ca2+-induced Ca2+-release, from intracellular Ca2+ stores in rapid equilibrium with extracellular Ca2+. By combining [Ca2+]i measurements with a specific plaque secretion assay we demonstrate that oscillating cells exhibit a spontaneous exocytic secretory activity whereas the cells with stable [Ca2+]i do not, [Ca2+]i oscillations appear therefore to account for the high unstimulated catecholamine release, an activity typical of the chromaffin cells of the rat.

Original languageEnglish
Pages (from-to)169-172
Number of pages4
JournalFEBS Letters
Volume283
Issue number2
DOIs
Publication statusPublished - Jun 3 1991

Keywords

  • Ca
  • Ca-induced Ca-release
  • Chromaffin cell
  • Exocytosis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

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