Cationic liposomes, loaded with m-THPC, in photodynamic therapy for malignant glioma

A. Molinari, M. Colone, A. Calcabrini, A. Stringaro, L. Toccacieli, G. Arancia, S. Mannino, A. Mangiola, G. Maira, C. Bombelli, G. Mancini

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Abstract

In this study we investigated the feasibility of mixed liposomes formed by dimyristoyl-sn-glycero-phosphatidylcholine (DMPC) and cationic gemini surfactant (Gemini 1) loaded with the chlorin m-tetrahydroxyphenylchlorin (m-THPC), in photodynamic therapy (PDT) for glioma. To this aim, an in vitro study was carried out by employing various human glioblastoma cell lines (A172, DBTRG, LN229, U118). The following liposomal formulations were tested: (i) DMPC and Gemini 1; (ii) m-THPC in DMPC in the absence or (iii) in the presence of Gemini 1 in the molar ratio 8:2; 7:3, and 6:4. The presence of Gemini 1 significantly increased the intracellular uptake of chlorin in all cell tested although with a different extent: LN229 > U118 > A172 > DBTRG. The cytotoxicity of chlorin-loaded liposomes was then tested by cloning efficiency performed on different cultures, before and after irradiation with laser light at 652 nm, at a Fluence Rate of 200 mW/s for 100 s, with a total Fluence of 20 J/cm-2. In the absence of irradiation, the different liposomal formulations induced a cytotoxicity in less than 30% of glioblastoma cells. On the contrary, irradiation induced total destruction of all cultures treated with m-THPC/DMPC + Gemini 1 in the ratios 8:2, or 7:3, or 6:4.

Original languageEnglish
Pages (from-to)230-234
Number of pages5
JournalToxicology in Vitro
Volume21
Issue number2
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Photodynamic therapy
Photochemotherapy
Phosphatidylcholines
Liposomes
Glioma
Irradiation
Glioblastoma
Cytotoxicity
Cloning
Cationic surfactants
Feasibility Studies
Surface-Active Agents
Organism Cloning
Lasers
Cells
Light
Cell Line
chlorin

Keywords

  • Cationic liposomes
  • Glioblastoma
  • m-THPC
  • Photodynamic therapy

ASJC Scopus subject areas

  • Toxicology

Cite this

Molinari, A., Colone, M., Calcabrini, A., Stringaro, A., Toccacieli, L., Arancia, G., ... Mancini, G. (2007). Cationic liposomes, loaded with m-THPC, in photodynamic therapy for malignant glioma. Toxicology in Vitro, 21(2), 230-234. https://doi.org/10.1016/j.tiv.2006.09.006

Cationic liposomes, loaded with m-THPC, in photodynamic therapy for malignant glioma. / Molinari, A.; Colone, M.; Calcabrini, A.; Stringaro, A.; Toccacieli, L.; Arancia, G.; Mannino, S.; Mangiola, A.; Maira, G.; Bombelli, C.; Mancini, G.

In: Toxicology in Vitro, Vol. 21, No. 2, 03.2007, p. 230-234.

Research output: Contribution to journalArticle

Molinari, A, Colone, M, Calcabrini, A, Stringaro, A, Toccacieli, L, Arancia, G, Mannino, S, Mangiola, A, Maira, G, Bombelli, C & Mancini, G 2007, 'Cationic liposomes, loaded with m-THPC, in photodynamic therapy for malignant glioma', Toxicology in Vitro, vol. 21, no. 2, pp. 230-234. https://doi.org/10.1016/j.tiv.2006.09.006
Molinari A, Colone M, Calcabrini A, Stringaro A, Toccacieli L, Arancia G et al. Cationic liposomes, loaded with m-THPC, in photodynamic therapy for malignant glioma. Toxicology in Vitro. 2007 Mar;21(2):230-234. https://doi.org/10.1016/j.tiv.2006.09.006
Molinari, A. ; Colone, M. ; Calcabrini, A. ; Stringaro, A. ; Toccacieli, L. ; Arancia, G. ; Mannino, S. ; Mangiola, A. ; Maira, G. ; Bombelli, C. ; Mancini, G. / Cationic liposomes, loaded with m-THPC, in photodynamic therapy for malignant glioma. In: Toxicology in Vitro. 2007 ; Vol. 21, No. 2. pp. 230-234.
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AU - Arancia, G.

AU - Mannino, S.

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