Abstract
Nuclear factor κB (NF-κB) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-κB after myocardial infarction. For the first time, we performed serial, non-invasive in vivo molecular imaging of transcription factor activation in the heart. We used mice expressing a luciferase reporter whose transcription is dependent upon NF-κB activation for up to 8 weeks after myocardial infarction. There was a significant increase of NF-κB activity with a maximum at day 3 after myocardial infarction when compared to sham controls. Thus, in vivo measurement of the activation of NF-κB is feasible. NF-κB activity might play an important role for the remodeling process.
| Original language | English |
|---|---|
| Pages (from-to) | 773-774 |
| Number of pages | 2 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 342 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Apr 14 2006 |
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Keywords
- Inflammation
- Myocardial infarction
- Nuclear factor κB
- Remodeling
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Caught in the act : In vivo molecular imaging of the transcription factor NF-κB after myocardial infarction. / Tillmanns, Jochen; Carlsen, Harald; Blomhoff, Rune; Valen, Guro; Calvillo, Laura; Ertl, Georg; Bauersachs, Johann; Frantz, Stefan.
In: Biochemical and Biophysical Research Communications, Vol. 342, No. 3, 14.04.2006, p. 773-774.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Caught in the act
T2 - In vivo molecular imaging of the transcription factor NF-κB after myocardial infarction
AU - Tillmanns, Jochen
AU - Carlsen, Harald
AU - Blomhoff, Rune
AU - Valen, Guro
AU - Calvillo, Laura
AU - Ertl, Georg
AU - Bauersachs, Johann
AU - Frantz, Stefan
PY - 2006/4/14
Y1 - 2006/4/14
N2 - Nuclear factor κB (NF-κB) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-κB after myocardial infarction. For the first time, we performed serial, non-invasive in vivo molecular imaging of transcription factor activation in the heart. We used mice expressing a luciferase reporter whose transcription is dependent upon NF-κB activation for up to 8 weeks after myocardial infarction. There was a significant increase of NF-κB activity with a maximum at day 3 after myocardial infarction when compared to sham controls. Thus, in vivo measurement of the activation of NF-κB is feasible. NF-κB activity might play an important role for the remodeling process.
AB - Nuclear factor κB (NF-κB) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-κB after myocardial infarction. For the first time, we performed serial, non-invasive in vivo molecular imaging of transcription factor activation in the heart. We used mice expressing a luciferase reporter whose transcription is dependent upon NF-κB activation for up to 8 weeks after myocardial infarction. There was a significant increase of NF-κB activity with a maximum at day 3 after myocardial infarction when compared to sham controls. Thus, in vivo measurement of the activation of NF-κB is feasible. NF-κB activity might play an important role for the remodeling process.
KW - Inflammation
KW - Myocardial infarction
KW - Nuclear factor κB
KW - Remodeling
UR - http://www.scopus.com/inward/record.url?scp=33644633047&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644633047&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.02.024
DO - 10.1016/j.bbrc.2006.02.024
M3 - Article
C2 - 16497270
AN - SCOPUS:33644633047
VL - 342
SP - 773
EP - 774
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -