Caught in the act: In vivo molecular imaging of the transcription factor NF-κB after myocardial infarction

Jochen Tillmanns, Harald Carlsen, Rune Blomhoff, Guro Valen, Laura Calvillo, Georg Ertl, Johann Bauersachs, Stefan Frantz

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Nuclear factor κB (NF-κB) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-κB after myocardial infarction. For the first time, we performed serial, non-invasive in vivo molecular imaging of transcription factor activation in the heart. We used mice expressing a luciferase reporter whose transcription is dependent upon NF-κB activation for up to 8 weeks after myocardial infarction. There was a significant increase of NF-κB activity with a maximum at day 3 after myocardial infarction when compared to sham controls. Thus, in vivo measurement of the activation of NF-κB is feasible. NF-κB activity might play an important role for the remodeling process.

Original languageEnglish
Pages (from-to)773-774
Number of pages2
JournalBiochemical and Biophysical Research Communications
Volume342
Issue number3
DOIs
Publication statusPublished - Apr 14 2006

Fingerprint

Molecular imaging
Molecular Imaging
Transcription Factors
Chemical activation
Myocardial Infarction
Heart Failure
Luciferases
Transcriptional Activation
Transcription

Keywords

  • Inflammation
  • Myocardial infarction
  • Nuclear factor κB
  • Remodeling

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Caught in the act : In vivo molecular imaging of the transcription factor NF-κB after myocardial infarction. / Tillmanns, Jochen; Carlsen, Harald; Blomhoff, Rune; Valen, Guro; Calvillo, Laura; Ertl, Georg; Bauersachs, Johann; Frantz, Stefan.

In: Biochemical and Biophysical Research Communications, Vol. 342, No. 3, 14.04.2006, p. 773-774.

Research output: Contribution to journalArticle

Tillmanns, Jochen ; Carlsen, Harald ; Blomhoff, Rune ; Valen, Guro ; Calvillo, Laura ; Ertl, Georg ; Bauersachs, Johann ; Frantz, Stefan. / Caught in the act : In vivo molecular imaging of the transcription factor NF-κB after myocardial infarction. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 342, No. 3. pp. 773-774.
@article{459b62b8aed3422aacfed822c629768b,
title = "Caught in the act: In vivo molecular imaging of the transcription factor NF-κB after myocardial infarction",
abstract = "Nuclear factor κB (NF-κB) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-κB after myocardial infarction. For the first time, we performed serial, non-invasive in vivo molecular imaging of transcription factor activation in the heart. We used mice expressing a luciferase reporter whose transcription is dependent upon NF-κB activation for up to 8 weeks after myocardial infarction. There was a significant increase of NF-κB activity with a maximum at day 3 after myocardial infarction when compared to sham controls. Thus, in vivo measurement of the activation of NF-κB is feasible. NF-κB activity might play an important role for the remodeling process.",
keywords = "Inflammation, Myocardial infarction, Nuclear factor κB, Remodeling",
author = "Jochen Tillmanns and Harald Carlsen and Rune Blomhoff and Guro Valen and Laura Calvillo and Georg Ertl and Johann Bauersachs and Stefan Frantz",
year = "2006",
month = "4",
day = "14",
doi = "10.1016/j.bbrc.2006.02.024",
language = "English",
volume = "342",
pages = "773--774",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Caught in the act

T2 - In vivo molecular imaging of the transcription factor NF-κB after myocardial infarction

AU - Tillmanns, Jochen

AU - Carlsen, Harald

AU - Blomhoff, Rune

AU - Valen, Guro

AU - Calvillo, Laura

AU - Ertl, Georg

AU - Bauersachs, Johann

AU - Frantz, Stefan

PY - 2006/4/14

Y1 - 2006/4/14

N2 - Nuclear factor κB (NF-κB) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-κB after myocardial infarction. For the first time, we performed serial, non-invasive in vivo molecular imaging of transcription factor activation in the heart. We used mice expressing a luciferase reporter whose transcription is dependent upon NF-κB activation for up to 8 weeks after myocardial infarction. There was a significant increase of NF-κB activity with a maximum at day 3 after myocardial infarction when compared to sham controls. Thus, in vivo measurement of the activation of NF-κB is feasible. NF-κB activity might play an important role for the remodeling process.

AB - Nuclear factor κB (NF-κB) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-κB after myocardial infarction. For the first time, we performed serial, non-invasive in vivo molecular imaging of transcription factor activation in the heart. We used mice expressing a luciferase reporter whose transcription is dependent upon NF-κB activation for up to 8 weeks after myocardial infarction. There was a significant increase of NF-κB activity with a maximum at day 3 after myocardial infarction when compared to sham controls. Thus, in vivo measurement of the activation of NF-κB is feasible. NF-κB activity might play an important role for the remodeling process.

KW - Inflammation

KW - Myocardial infarction

KW - Nuclear factor κB

KW - Remodeling

UR - http://www.scopus.com/inward/record.url?scp=33644633047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644633047&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.02.024

DO - 10.1016/j.bbrc.2006.02.024

M3 - Article

C2 - 16497270

AN - SCOPUS:33644633047

VL - 342

SP - 773

EP - 774

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -