TY - JOUR
T1 - Caught in the act
T2 - In vivo molecular imaging of the transcription factor NF-κB after myocardial infarction
AU - Tillmanns, Jochen
AU - Carlsen, Harald
AU - Blomhoff, Rune
AU - Valen, Guro
AU - Calvillo, Laura
AU - Ertl, Georg
AU - Bauersachs, Johann
AU - Frantz, Stefan
PY - 2006/4/14
Y1 - 2006/4/14
N2 - Nuclear factor κB (NF-κB) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-κB after myocardial infarction. For the first time, we performed serial, non-invasive in vivo molecular imaging of transcription factor activation in the heart. We used mice expressing a luciferase reporter whose transcription is dependent upon NF-κB activation for up to 8 weeks after myocardial infarction. There was a significant increase of NF-κB activity with a maximum at day 3 after myocardial infarction when compared to sham controls. Thus, in vivo measurement of the activation of NF-κB is feasible. NF-κB activity might play an important role for the remodeling process.
AB - Nuclear factor κB (NF-κB) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-κB after myocardial infarction. For the first time, we performed serial, non-invasive in vivo molecular imaging of transcription factor activation in the heart. We used mice expressing a luciferase reporter whose transcription is dependent upon NF-κB activation for up to 8 weeks after myocardial infarction. There was a significant increase of NF-κB activity with a maximum at day 3 after myocardial infarction when compared to sham controls. Thus, in vivo measurement of the activation of NF-κB is feasible. NF-κB activity might play an important role for the remodeling process.
KW - Inflammation
KW - Myocardial infarction
KW - Nuclear factor κB
KW - Remodeling
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U2 - 10.1016/j.bbrc.2006.02.024
DO - 10.1016/j.bbrc.2006.02.024
M3 - Article
C2 - 16497270
AN - SCOPUS:33644633047
VL - 342
SP - 773
EP - 774
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -