Caveolin-1 overexpression is associated with simultaneous abnormal expression of the E-cadherin/α-β catenins complex and multiple erbb receptors and with lymph nodes metastasis in head and neck squamous cell carcinomas

Laura Masuelli, Alfredo Budillon, Laura Marzocchella, Marie Agnes Mrozek, Domenico Vitolo, Elena Di Gennaro, Simona Losito, Patrizio Sale, Francesco Longo, Franco Ionna, Florigio Lista, Raffaella Muraro, Andrea Modesti, Roberto Bei

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The presence of lymph node metastases is one of the most important prognostic indicators in head and neck squamous cell carcinomas (HNSCCs). An alteration of the E-cadherin-catenins complex and EGFR is essential for the invasiveness of cancer cells. Caveolin-1, the major structural protein of the caveolae, represents a scaffolding molecule for several signaling proteins including EGFR. Although caveolin-1 has been shown to play a role in inducing the invasive phenotype of cancer cells, its role appears to be cell-type specific and for some tumors it has not been defined yet. In this study we used 57 HNSCC specimens to investigate whether the abnormal expression of caveolin-1 was associated with the derangement of the E-cadherin-catenins complex and with the overexpression of ErbB receptors. We demonstrate that in HNSCCs caveolin-1 overexpression is associated with the simultaneous abnormal expression of at least one member of the E-cadherin/α-β catenins complex and multiple ErbB receptors as well as with lymph node metastases. We also demonstrate that chronic stimulation of a human hypopharyngeal carcinoma cell line (FaDu) with EGF induced the internalization of β-catenin and caveolin-1 and their co-localization with EGFR. Moreover, EGF treatment induced an increased physical interaction between EGFR/β-catenin/caveolin-1 and between E-cadherin/β-catenin/caveolin-1. These molecular events were associated with an increased directional motility of FaDu cells in vitro. These findings may provide new insight into the biology of HNSCC progression and help to identify subgroups of primary HNSCCs with a more aggressive behavior.

Original languageEnglish
Pages (from-to)3344-3353
Number of pages10
JournalJournal of Cellular Physiology
Volume227
Issue number9
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Caveolin 1
Catenins
Cadherins
Lymph Nodes
Neoplasm Metastasis
Cells
Epidermal Growth Factor
Caveolae
Neoplasms
ErbB Receptors
Carcinoma, squamous cell of head and neck
Epithelial Cells
Cell Movement
Tumors
Proteins
Carcinoma
Phenotype
Cell Line
Molecules

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Caveolin-1 overexpression is associated with simultaneous abnormal expression of the E-cadherin/α-β catenins complex and multiple erbb receptors and with lymph nodes metastasis in head and neck squamous cell carcinomas. / Masuelli, Laura; Budillon, Alfredo; Marzocchella, Laura; Mrozek, Marie Agnes; Vitolo, Domenico; Di Gennaro, Elena; Losito, Simona; Sale, Patrizio; Longo, Francesco; Ionna, Franco; Lista, Florigio; Muraro, Raffaella; Modesti, Andrea; Bei, Roberto.

In: Journal of Cellular Physiology, Vol. 227, No. 9, 09.2012, p. 3344-3353.

Research output: Contribution to journalArticle

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AU - Sale, Patrizio

AU - Longo, Francesco

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