CBP-independent activation of CREM and CREB by the LIM-only protein ACT

Gian Maria Fimia, Dario De Cesare, Paolo Sassone-Corsi

Research output: Contribution to journalArticle

Abstract

Transcriptional activation by CREB and CREM requires phosphorylation of a serine residue within the activation domain (Ser 133 in CREB; Ser 117 in CREM) which as a result interacts with the coactivator CBP. The activator CREM is highly expressed in male germ cells and is required for post-meiotic gene expression. Using a two-hybrid screen, we have isolated a testis- derived complementary DNA encoding a protein that we term ACT (for activator of CREM in testis), a LIM-only protein which specifically associates with CREM. ACT is expressed coordinately with CREM in a tissue- and developmentally regulated manner. It strongly stimulates CREM transcriptional activity in yeast and mammalian cells and contains an intrinsic activation function. As ACT bypasses the classical requirements for activation, namely phosphorylation of Ser 117 and interaction with CBP, it represents a new route for transcriptional activation by CREM and CREB. ACT may define a previously undiscovered class of tissue-specific coactivators whose function could be specific for distinct cellular differentiation programmes.

Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalNature
Volume398
Issue number6723
DOIs
Publication statusPublished - Mar 11 1999

ASJC Scopus subject areas

  • General

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