CB2 and TRPV1 receptors oppositely modulate in vitro human osteoblast activity

Francesca Rossi, Giulia Bellini, Chiara Tortora, Maria Ester Bernardo, Livio Luongo, Antonella Conforti, Nadia Starc, Iolanda Manzo, Bruno Nobili, Franco Locatelli, Sabatino Maione

Research output: Contribution to journalArticle

Abstract

Abstract In the current study, we have investigated the effect of CB2 and TRPV1 receptor ligands on in vitro osteoblasts from bone marrow of human healthy donors. A pivotal role for the endocannabinoid/endovanilloid system in bone metabolism has been highlighted. We have demonstrated a functional cross-talk between CB2 and TRPV1 in human osteoclasts, suggesting these receptors as new pharmacological target for the treatment of bone resorption disease as osteoporosis. Moreover, we have shown the presence of these receptors on human mesenchimal stem cells, hMSCs. Osteoblasts are mononucleated cells originated from hMSCs by the essential transcription factor runt-related transcription factor 2 and involved in bone formation via the synthesis and release of macrophage colony-stimulating factor, receptor activator of nuclear factor kappa-B ligand and osteoprotegerin. For the first time, we show that CB2 and TRPV1 receptors are both expressed on human osteoblasts together with enzymes synthesizing and degrading endocannabinoids/endovanilloids, and oppositely modulate human osteoblast activity in culture in a way that the CB2 receptor stimulation improves the osteogenesis whereas TRPV1 receptor stimulation inhibits it.

Original languageEnglish
Article number2853
Pages (from-to)194-201
Number of pages8
JournalPharmacological Research
Volume99
DOIs
Publication statusPublished - Jul 14 2015

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Keywords

  • CB<inf>2</inf> receptor
  • Endocannabinoid/endovanilloid system
  • Human osteoblast culture
  • Osteoblast activity markers
  • TRPV<inf>1</inf> receptor

ASJC Scopus subject areas

  • Pharmacology

Cite this

Rossi, F., Bellini, G., Tortora, C., Bernardo, M. E., Luongo, L., Conforti, A., Starc, N., Manzo, I., Nobili, B., Locatelli, F., & Maione, S. (2015). CB2 and TRPV1 receptors oppositely modulate in vitro human osteoblast activity. Pharmacological Research, 99, 194-201. [2853]. https://doi.org/10.1016/j.phrs.2015.06.010