CCL16 activates an angiogenic program in vascular endothelial cells

Marina Strasly, Gabriella Doronzo, Paola Cappello, Donatella Valdembri, Marco Arese, Stefania Mitola, Paul Moore, Giulio Alessandri, Mirella Giovarelli, Federico Bussolino

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Besides regulating leukocyte trafficking in normal and injured tissues, several chemokines may positively or negatively regulate angiogenesis. Here we report that CCL16 activates an angiogenic program in vascular endothelial cells by activating CCR1. CCL16 induces dose-dependent random and directional migration of endothelial cells isolated from large vessels and liver capillaries without inducing their proliferation. It also promotes endothelial differentiation into capillary-like structures in an in vitro assay and is angiogenic in the chick chorionallantoic membrane. These angiogenic activities are neutralized by a specific antibody against CCL16. The direct angiogenic activity of CCL16 is further amplified by its ability to prime endothelium to a mitogen signal induced by vascular endothelial growth factor A and to raise their basal production of CXCL8 and CCL2, 2 other angiogenic chemokines. BX471 (R-N-[5-chloro-2-[2-[4(4-fluorophenyl) methyl]-2-methyl-1-piperazinyl]-2-oxoethoxy]phenyl] urea hydrochloric acid salt), a CCR1 antagonist, inhibits angiogenic properties of CCL16, whereas blocking of CCR8 or desensitizing CCR2, which are both well known receptors for CCL16, did not abolish endothelial activation. CCL16 may be specifically cross-linked to CCR1 expressed on endothelial cells. The largely restricted CCL16 expression in the liver suggests that this chemokine may play a role in hepatic vascular formation during development and in angiogenesis associated to hepatic diseases.

Original languageEnglish
Pages (from-to)40-49
Number of pages10
JournalBlood
Volume103
Issue number1
DOIs
Publication statusPublished - Jan 1 2004

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Endothelial cells
Chemokines
Endothelial Cells
Liver
Mitogens
Vascular Endothelial Growth Factor A
Angiogenesis Inhibitors
Assays
Chemical activation
Tissue
Endothelium
Membranes
Blood Vessels
Leukocytes
Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

CCL16 activates an angiogenic program in vascular endothelial cells. / Strasly, Marina; Doronzo, Gabriella; Cappello, Paola; Valdembri, Donatella; Arese, Marco; Mitola, Stefania; Moore, Paul; Alessandri, Giulio; Giovarelli, Mirella; Bussolino, Federico.

In: Blood, Vol. 103, No. 1, 01.01.2004, p. 40-49.

Research output: Contribution to journalArticle

Strasly, M, Doronzo, G, Cappello, P, Valdembri, D, Arese, M, Mitola, S, Moore, P, Alessandri, G, Giovarelli, M & Bussolino, F 2004, 'CCL16 activates an angiogenic program in vascular endothelial cells', Blood, vol. 103, no. 1, pp. 40-49. https://doi.org/10.1182/blood-2003-05-1387
Strasly, Marina ; Doronzo, Gabriella ; Cappello, Paola ; Valdembri, Donatella ; Arese, Marco ; Mitola, Stefania ; Moore, Paul ; Alessandri, Giulio ; Giovarelli, Mirella ; Bussolino, Federico. / CCL16 activates an angiogenic program in vascular endothelial cells. In: Blood. 2004 ; Vol. 103, No. 1. pp. 40-49.
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