CCR7 is involved in the migration of neutrophils to lymph nodes

Céline Beauvillain, Pierre Cunin, Andrea Doni, Mari Scotet, Sébastien Jaillon, Marie Line Loiry, Giovanni Magistrelli, Krzysztof Masternak, Alain Chevailler, Yves Delneste, Pascale Jeannin

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Increasing evidence suggests that neutrophils may participate in the regulation of adaptive immune responses, and can reach draining lymph nodes and crossprime naive T cells. The aim of this study was to identify the mechanism(s) involved in the migration of neutrophils to the draining lymph nodes. We demonstrate that a subpopulation of human and mouse neutrophils express CCR7. CCR7 is rapidly expressed at the membrane upon stimulation. In vitro, stimulated human neutrophils migrate in response to the CCR7 ligands CCL19 and CCL21. In vivo, injection of complete Freund adjuvant induces a rapid recruitment of neutrophils to the lymph nodes in wild-type mice but not in Ccr7-/- mice. Moreover, intradermally injected interleukin-17 - and granulocyte- macrophage colonystimulating factor - stimulated neutrophils from wild-type mice, but not from Ccr7-/- mice, migrate to the draining lymph nodes. These results identify CCR7 as a chemokine receptor involved in the migration of neutrophils to the lymph nodes.

Original languageEnglish
Pages (from-to)1196-1204
Number of pages9
JournalBlood
Volume117
Issue number4
DOIs
Publication statusPublished - Jan 27 2011

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T-cells
Interleukin-17
Freund's Adjuvant
Macrophages
Chemokine Receptors
Neutrophils
Lymph Nodes
Ligands
Membranes
Neutrophil Infiltration
Adaptive Immunity
Granulocytes
T-Lymphocytes
Injections

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Beauvillain, C., Cunin, P., Doni, A., Scotet, M., Jaillon, S., Loiry, M. L., ... Jeannin, P. (2011). CCR7 is involved in the migration of neutrophils to lymph nodes. Blood, 117(4), 1196-1204. https://doi.org/10.1182/blood-2009-11-254490

CCR7 is involved in the migration of neutrophils to lymph nodes. / Beauvillain, Céline; Cunin, Pierre; Doni, Andrea; Scotet, Mari; Jaillon, Sébastien; Loiry, Marie Line; Magistrelli, Giovanni; Masternak, Krzysztof; Chevailler, Alain; Delneste, Yves; Jeannin, Pascale.

In: Blood, Vol. 117, No. 4, 27.01.2011, p. 1196-1204.

Research output: Contribution to journalArticle

Beauvillain, C, Cunin, P, Doni, A, Scotet, M, Jaillon, S, Loiry, ML, Magistrelli, G, Masternak, K, Chevailler, A, Delneste, Y & Jeannin, P 2011, 'CCR7 is involved in the migration of neutrophils to lymph nodes', Blood, vol. 117, no. 4, pp. 1196-1204. https://doi.org/10.1182/blood-2009-11-254490
Beauvillain, Céline ; Cunin, Pierre ; Doni, Andrea ; Scotet, Mari ; Jaillon, Sébastien ; Loiry, Marie Line ; Magistrelli, Giovanni ; Masternak, Krzysztof ; Chevailler, Alain ; Delneste, Yves ; Jeannin, Pascale. / CCR7 is involved in the migration of neutrophils to lymph nodes. In: Blood. 2011 ; Vol. 117, No. 4. pp. 1196-1204.
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