CD103 marks a subset of human CD34+-derived langerin+ dendritic cells that induce T-regulatory cells via indoleamine 2,3-dioxygenase-1

Darina Očadlíková, Sara Trabanelli, Valentina Salvestrini, Marilena Ciciarello, Cecilia Evangelisti, Mariangela Lecciso, Elena Sabattini, Simona Righi, Milena Piccioli, Stefano A. Pileri, Roberto M. Lemoli, Antonio Curti

Research output: Contribution to journalArticle

Abstract

Indoleamine 2,3-dioxygenase 1 (IDO1) is an immunosuppressive molecule expressed in some subsets of normal and neoplastic cells. Mature human dendritic cells (DCs) have been shown to express IDO1, but little is known about its expression and function during DC differentiation from bone marrow hematopoietic stem/progenitor cells (HSPCs). Here, we show that during invitro differentiation along the myeloid DC lineage, CD34+ HSPCs acquire IDO1 expression, which acts in a tolerogenic manner by inducing a population of fully functional CD4+CD25+ FOXP3+ T-regulatory cells. Phenotypically, CD1a+CD14- HPSC-derived DCs expressed IDO1, langerin, CD11b, and CD1c. Cell-sorting experiments demonstrated that IDO1 expression is found in a subset of CD1a+CD14-langerin+ cells, expressing CD103, which is capable of inducing T-regulatory cells in an IDO1-dependent manner. In conclusion, DC differentiation from CD34+ HSPCs results in the expression of a functionally active IDO1 protein in CD1a+langerin+, CD103-expressing DCs. These data point toward IDO1 expression as part of a tolerogenic signature during DC development.

Original languageEnglish
Pages (from-to)268-276
Number of pages9
JournalExperimental Hematology
Volume43
Issue number4
DOIs
Publication statusPublished - Apr 1 2015

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Indoleamine-Pyrrole 2,3,-Dioxygenase
Regulatory T-Lymphocytes
Dendritic Cells
Hematopoietic Stem Cells
Cell Differentiation
Cell Lineage
Myeloid Cells
Immunosuppressive Agents
Bone Marrow

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Hematology
  • Medicine(all)

Cite this

Očadlíková, D., Trabanelli, S., Salvestrini, V., Ciciarello, M., Evangelisti, C., Lecciso, M., ... Curti, A. (2015). CD103 marks a subset of human CD34+-derived langerin+ dendritic cells that induce T-regulatory cells via indoleamine 2,3-dioxygenase-1. Experimental Hematology, 43(4), 268-276. https://doi.org/10.1016/j.exphem.2014.12.007

CD103 marks a subset of human CD34+-derived langerin+ dendritic cells that induce T-regulatory cells via indoleamine 2,3-dioxygenase-1. / Očadlíková, Darina; Trabanelli, Sara; Salvestrini, Valentina; Ciciarello, Marilena; Evangelisti, Cecilia; Lecciso, Mariangela; Sabattini, Elena; Righi, Simona; Piccioli, Milena; Pileri, Stefano A.; Lemoli, Roberto M.; Curti, Antonio.

In: Experimental Hematology, Vol. 43, No. 4, 01.04.2015, p. 268-276.

Research output: Contribution to journalArticle

Očadlíková, D, Trabanelli, S, Salvestrini, V, Ciciarello, M, Evangelisti, C, Lecciso, M, Sabattini, E, Righi, S, Piccioli, M, Pileri, SA, Lemoli, RM & Curti, A 2015, 'CD103 marks a subset of human CD34+-derived langerin+ dendritic cells that induce T-regulatory cells via indoleamine 2,3-dioxygenase-1', Experimental Hematology, vol. 43, no. 4, pp. 268-276. https://doi.org/10.1016/j.exphem.2014.12.007
Očadlíková, Darina ; Trabanelli, Sara ; Salvestrini, Valentina ; Ciciarello, Marilena ; Evangelisti, Cecilia ; Lecciso, Mariangela ; Sabattini, Elena ; Righi, Simona ; Piccioli, Milena ; Pileri, Stefano A. ; Lemoli, Roberto M. ; Curti, Antonio. / CD103 marks a subset of human CD34+-derived langerin+ dendritic cells that induce T-regulatory cells via indoleamine 2,3-dioxygenase-1. In: Experimental Hematology. 2015 ; Vol. 43, No. 4. pp. 268-276.
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