Abstract
Emerging evidence suggests that tumors contain and are driven by a cellular component that displays stem cell properties, the so-called cancer stem cells (CSCs). CSCs have been identified in several solid human cancers; however, there are no data about CSCs in primary human gastric cancer (GC). By using CD133 and CD44 cell surface markers we investigated whether primary human GCs contain a cell subset expressing stem-like properties and whether this subpopulation has tumor-initiating properties in xenograft transplantation experiments. We examined tissues from 44 patients who underwent gastrectomy for primary GC. The tumorigenicity of the cells separated by flow cytometry using CD133 and CD44 surface markers was tested by subcutaneous or intraperitoneum injection in NOD/SCID and nude mice. GCs included in the study were intestinal in 34 cases and diffuse in 10 cases. All samples contained surface marker-positive cells: CD133 + mean percentage 10.6% and CD133 +/CD44 + mean percentage 27.7%, irrespective of cancer phenotype or grade of differentiation. Purified CD133 + and CD133 +/CD44 + cells, obtained in sufficient number only in 12 intestinal type GC cases, failed to reproduce cancer in two mice models. However, the unseparated cells produced glandular-like structures in 70% of the mice inoculated. In conclusion, although CD133 + and CD133 +/CD44 + were detectable in human primary GCs, they neither expressed stem-like properties nor exhibited tumor-initiating properties in xenograft transplantation experiments.
| Original language | English |
|---|---|
| Pages (from-to) | 2686-2693 |
| Number of pages | 8 |
| Journal | Journal of Cellular Physiology |
| Volume | 227 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2012 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
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CD133 and CD44 Cell surface markers do not identify cancer stem cells in primary human gastric tumors. / Rocco, Alba; Liguori, Eleonora; Pirozzi, Giuseppe; Tirino, Virginia; Compare, Debora; Franco, Renato; Tatangelo, Fabiana; Palaia, Raffaele; D'Armiento, Francesco Paolo; Pollastrone, Giorgia; Affuso, Andrea; Bottazzi, Enrico Coppola; Masone, Stefania; Persico, Giovanni; Nardone, Gerardo.
In: Journal of Cellular Physiology, Vol. 227, No. 6, 06.2012, p. 2686-2693.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CD133 and CD44 Cell surface markers do not identify cancer stem cells in primary human gastric tumors
AU - Rocco, Alba
AU - Liguori, Eleonora
AU - Pirozzi, Giuseppe
AU - Tirino, Virginia
AU - Compare, Debora
AU - Franco, Renato
AU - Tatangelo, Fabiana
AU - Palaia, Raffaele
AU - D'Armiento, Francesco Paolo
AU - Pollastrone, Giorgia
AU - Affuso, Andrea
AU - Bottazzi, Enrico Coppola
AU - Masone, Stefania
AU - Persico, Giovanni
AU - Nardone, Gerardo
PY - 2012/6
Y1 - 2012/6
N2 - Emerging evidence suggests that tumors contain and are driven by a cellular component that displays stem cell properties, the so-called cancer stem cells (CSCs). CSCs have been identified in several solid human cancers; however, there are no data about CSCs in primary human gastric cancer (GC). By using CD133 and CD44 cell surface markers we investigated whether primary human GCs contain a cell subset expressing stem-like properties and whether this subpopulation has tumor-initiating properties in xenograft transplantation experiments. We examined tissues from 44 patients who underwent gastrectomy for primary GC. The tumorigenicity of the cells separated by flow cytometry using CD133 and CD44 surface markers was tested by subcutaneous or intraperitoneum injection in NOD/SCID and nude mice. GCs included in the study were intestinal in 34 cases and diffuse in 10 cases. All samples contained surface marker-positive cells: CD133 + mean percentage 10.6% and CD133 +/CD44 + mean percentage 27.7%, irrespective of cancer phenotype or grade of differentiation. Purified CD133 + and CD133 +/CD44 + cells, obtained in sufficient number only in 12 intestinal type GC cases, failed to reproduce cancer in two mice models. However, the unseparated cells produced glandular-like structures in 70% of the mice inoculated. In conclusion, although CD133 + and CD133 +/CD44 + were detectable in human primary GCs, they neither expressed stem-like properties nor exhibited tumor-initiating properties in xenograft transplantation experiments.
AB - Emerging evidence suggests that tumors contain and are driven by a cellular component that displays stem cell properties, the so-called cancer stem cells (CSCs). CSCs have been identified in several solid human cancers; however, there are no data about CSCs in primary human gastric cancer (GC). By using CD133 and CD44 cell surface markers we investigated whether primary human GCs contain a cell subset expressing stem-like properties and whether this subpopulation has tumor-initiating properties in xenograft transplantation experiments. We examined tissues from 44 patients who underwent gastrectomy for primary GC. The tumorigenicity of the cells separated by flow cytometry using CD133 and CD44 surface markers was tested by subcutaneous or intraperitoneum injection in NOD/SCID and nude mice. GCs included in the study were intestinal in 34 cases and diffuse in 10 cases. All samples contained surface marker-positive cells: CD133 + mean percentage 10.6% and CD133 +/CD44 + mean percentage 27.7%, irrespective of cancer phenotype or grade of differentiation. Purified CD133 + and CD133 +/CD44 + cells, obtained in sufficient number only in 12 intestinal type GC cases, failed to reproduce cancer in two mice models. However, the unseparated cells produced glandular-like structures in 70% of the mice inoculated. In conclusion, although CD133 + and CD133 +/CD44 + were detectable in human primary GCs, they neither expressed stem-like properties nor exhibited tumor-initiating properties in xenograft transplantation experiments.
UR - http://www.scopus.com/inward/record.url?scp=84857474200&partnerID=8YFLogxK
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U2 - 10.1002/jcp.23013
DO - 10.1002/jcp.23013
M3 - Article
VL - 227
SP - 2686
EP - 2693
JO - Journal of cellular and comparative physiology
JF - Journal of cellular and comparative physiology
SN - 0021-9541
IS - 6
ER -