CD133 and CD44 Cell surface markers do not identify cancer stem cells in primary human gastric tumors

Alba Rocco, Eleonora Liguori, Giuseppe Pirozzi, Virginia Tirino, Debora Compare, Renato Franco, Fabiana Tatangelo, Raffaele Palaia, Francesco Paolo D'Armiento, Giorgia Pollastrone, Andrea Affuso, Enrico Coppola Bottazzi, Stefania Masone, Giovanni Persico, Gerardo Nardone

Research output: Contribution to journalArticle

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Abstract

Emerging evidence suggests that tumors contain and are driven by a cellular component that displays stem cell properties, the so-called cancer stem cells (CSCs). CSCs have been identified in several solid human cancers; however, there are no data about CSCs in primary human gastric cancer (GC). By using CD133 and CD44 cell surface markers we investigated whether primary human GCs contain a cell subset expressing stem-like properties and whether this subpopulation has tumor-initiating properties in xenograft transplantation experiments. We examined tissues from 44 patients who underwent gastrectomy for primary GC. The tumorigenicity of the cells separated by flow cytometry using CD133 and CD44 surface markers was tested by subcutaneous or intraperitoneum injection in NOD/SCID and nude mice. GCs included in the study were intestinal in 34 cases and diffuse in 10 cases. All samples contained surface marker-positive cells: CD133 + mean percentage 10.6% and CD133 +/CD44 + mean percentage 27.7%, irrespective of cancer phenotype or grade of differentiation. Purified CD133 + and CD133 +/CD44 + cells, obtained in sufficient number only in 12 intestinal type GC cases, failed to reproduce cancer in two mice models. However, the unseparated cells produced glandular-like structures in 70% of the mice inoculated. In conclusion, although CD133 + and CD133 +/CD44 + were detectable in human primary GCs, they neither expressed stem-like properties nor exhibited tumor-initiating properties in xenograft transplantation experiments.

Original languageEnglish
Pages (from-to)2686-2693
Number of pages8
JournalJournal of Cellular Physiology
Volume227
Issue number6
DOIs
Publication statusPublished - Jun 2012

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Neoplastic Stem Cells
Stem cells
Tumors
Stomach
Heterografts
Stomach Neoplasms
Neoplasms
Heterologous Transplantation
Flow cytometry
Intestinal Neoplasms
Inbred NOD Mouse
SCID Mice
Experiments
Cells
Gastrectomy
Tissue
Nude Mice
Flow Cytometry
Stem Cells
Phenotype

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Rocco, A., Liguori, E., Pirozzi, G., Tirino, V., Compare, D., Franco, R., ... Nardone, G. (2012). CD133 and CD44 Cell surface markers do not identify cancer stem cells in primary human gastric tumors. Journal of Cellular Physiology, 227(6), 2686-2693. https://doi.org/10.1002/jcp.23013

CD133 and CD44 Cell surface markers do not identify cancer stem cells in primary human gastric tumors. / Rocco, Alba; Liguori, Eleonora; Pirozzi, Giuseppe; Tirino, Virginia; Compare, Debora; Franco, Renato; Tatangelo, Fabiana; Palaia, Raffaele; D'Armiento, Francesco Paolo; Pollastrone, Giorgia; Affuso, Andrea; Bottazzi, Enrico Coppola; Masone, Stefania; Persico, Giovanni; Nardone, Gerardo.

In: Journal of Cellular Physiology, Vol. 227, No. 6, 06.2012, p. 2686-2693.

Research output: Contribution to journalArticle

Rocco, A, Liguori, E, Pirozzi, G, Tirino, V, Compare, D, Franco, R, Tatangelo, F, Palaia, R, D'Armiento, FP, Pollastrone, G, Affuso, A, Bottazzi, EC, Masone, S, Persico, G & Nardone, G 2012, 'CD133 and CD44 Cell surface markers do not identify cancer stem cells in primary human gastric tumors', Journal of Cellular Physiology, vol. 227, no. 6, pp. 2686-2693. https://doi.org/10.1002/jcp.23013
Rocco, Alba ; Liguori, Eleonora ; Pirozzi, Giuseppe ; Tirino, Virginia ; Compare, Debora ; Franco, Renato ; Tatangelo, Fabiana ; Palaia, Raffaele ; D'Armiento, Francesco Paolo ; Pollastrone, Giorgia ; Affuso, Andrea ; Bottazzi, Enrico Coppola ; Masone, Stefania ; Persico, Giovanni ; Nardone, Gerardo. / CD133 and CD44 Cell surface markers do not identify cancer stem cells in primary human gastric tumors. In: Journal of Cellular Physiology. 2012 ; Vol. 227, No. 6. pp. 2686-2693.
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AU - Compare, Debora

AU - Franco, Renato

AU - Tatangelo, Fabiana

AU - Palaia, Raffaele

AU - D'Armiento, Francesco Paolo

AU - Pollastrone, Giorgia

AU - Affuso, Andrea

AU - Bottazzi, Enrico Coppola

AU - Masone, Stefania

AU - Persico, Giovanni

AU - Nardone, Gerardo

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