CD1c-reactive, Th2 cytokine producing T-cells in human autoimmune disease

Peter A. Sieling, Steven A. Porcelli, Franca Spada, Marika Falcone, Barry R. Bloom, Betty Diamond, Robert L. Modlin

Research output: Contribution to journalArticle

Abstract

The role of CD1-restricted T cells in autoimmunity was investigated by studying patients with systemic lupus erythematosus (SLE). CD4-.CD8- double negative (DN) T-cell lines were derived from the peripheral blood of patients with SLE and from healthy donors in the presence of CD1+ antigen presenting cells. Double negative T-cell lines were restricted by CD1c and produced IL-4 but not IFN-γ. In contrast, CD1c-reactive T-cells from healthy donors produced no IL-4 and produced considerable amounts of IFN-γ. B-lymphoblastoid cell lines transfected with CD1c and CD1c+ peripheral blood B-cells induced IL-4 production from SLE-derived double negative T-cell lines, suggesting that cognate recognition of a self antigen could take place in the context of CD1c. Finally, CD1c-reactive T-cells responded to CD1c lacking an endosomal. targeting signal, indicating that antigen presentation is independent of endosomal processing. These data suggest that CD1c-reactive, Th2 T cells in SLE could interact with B cells and alter antibody responses in autoimmune disease.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
Publication statusPublished - Mar 20 1998

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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    Sieling, P. A., Porcelli, S. A., Spada, F., Falcone, M., Bloom, B. R., Diamond, B., & Modlin, R. L. (1998). CD1c-reactive, Th2 cytokine producing T-cells in human autoimmune disease. FASEB Journal, 12(5).