CD1d-restricted help to B cells by human invariant natural killer T lymphocytes

Grazia Galli, Sandra Nuti, Simona Tavarini, Luisa Galli-Stampino, Claudia De Lalla, Giulia Casorati, Paolo Dellabona, Sergio Abrignani

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

Invariant natural killer T (NKT) cells are a highly conserved subset of T lymphocytes expressing a semi-invariant T cell receptor (TCR), which is restricted to CDld and specific for the glycosphingolipid antigen α-galactosylceramide. Their ability to secrete a variety of cytokines, which in turn modulate the activation of cells of both innate and acquired immune responses, suggests that invariant NKT cells exert a regulatory role mainly via indirect mechanisms. A relevant question is whether invariant NKT cells can directly help B cells. We document here that human invariant NKT cells are as efficient as conventional CD4+ Th0 lymphocytes in promoting proliferation of autologous memory and naive B lymphocytes in vitro, and in inducing immunoglobulin production. Help to B cells by invariant NKT cells is CD1d-dependent and delivered also in the absence of α-galactosylceramide, suggesting that NKT cells recognize an endogenous ligand presented by CD1d on B cells. The two major subsets of invariant NKT cells, CD4+ and double negative (CD4-CD8-), express comparable levels of CD40 ligand and cytokines, but differ in helper functions. Indeed, both subsets induce similar levels of B cell proliferation, whereas CD4+ NKT cells induce higher levels of immunoglobulin production. These results suggest a direct role for invariant NKT cells in regulating B lymphocyte proliferation and effector functions.

Original languageEnglish
Pages (from-to)1051-1057
Number of pages7
JournalJournal of Experimental Medicine
Volume197
Issue number8
DOIs
Publication statusPublished - Apr 21 2003

Fingerprint

Natural Killer T-Cells
B-Lymphocytes
T-Lymphocytes
Galactosylceramides
Immunoglobulins
Cytokines
B-Lymphocyte Subsets
Glycosphingolipids
CD40 Ligand
Aptitude
T-Lymphocyte Subsets
T-Cell Antigen Receptor
Innate Immunity
Cell Proliferation
Lymphocytes
Ligands
Antigens

Keywords

  • α-galactosylceramide
  • Antibodies
  • Autoreactivity
  • Cytokine
  • Helper assay

ASJC Scopus subject areas

  • Immunology

Cite this

CD1d-restricted help to B cells by human invariant natural killer T lymphocytes. / Galli, Grazia; Nuti, Sandra; Tavarini, Simona; Galli-Stampino, Luisa; De Lalla, Claudia; Casorati, Giulia; Dellabona, Paolo; Abrignani, Sergio.

In: Journal of Experimental Medicine, Vol. 197, No. 8, 21.04.2003, p. 1051-1057.

Research output: Contribution to journalArticle

Galli, Grazia ; Nuti, Sandra ; Tavarini, Simona ; Galli-Stampino, Luisa ; De Lalla, Claudia ; Casorati, Giulia ; Dellabona, Paolo ; Abrignani, Sergio. / CD1d-restricted help to B cells by human invariant natural killer T lymphocytes. In: Journal of Experimental Medicine. 2003 ; Vol. 197, No. 8. pp. 1051-1057.
@article{5b5ceab1b4fe4b1b8eea03874f08a07d,
title = "CD1d-restricted help to B cells by human invariant natural killer T lymphocytes",
abstract = "Invariant natural killer T (NKT) cells are a highly conserved subset of T lymphocytes expressing a semi-invariant T cell receptor (TCR), which is restricted to CDld and specific for the glycosphingolipid antigen α-galactosylceramide. Their ability to secrete a variety of cytokines, which in turn modulate the activation of cells of both innate and acquired immune responses, suggests that invariant NKT cells exert a regulatory role mainly via indirect mechanisms. A relevant question is whether invariant NKT cells can directly help B cells. We document here that human invariant NKT cells are as efficient as conventional CD4+ Th0 lymphocytes in promoting proliferation of autologous memory and naive B lymphocytes in vitro, and in inducing immunoglobulin production. Help to B cells by invariant NKT cells is CD1d-dependent and delivered also in the absence of α-galactosylceramide, suggesting that NKT cells recognize an endogenous ligand presented by CD1d on B cells. The two major subsets of invariant NKT cells, CD4+ and double negative (CD4-CD8-), express comparable levels of CD40 ligand and cytokines, but differ in helper functions. Indeed, both subsets induce similar levels of B cell proliferation, whereas CD4+ NKT cells induce higher levels of immunoglobulin production. These results suggest a direct role for invariant NKT cells in regulating B lymphocyte proliferation and effector functions.",
keywords = "α-galactosylceramide, Antibodies, Autoreactivity, Cytokine, Helper assay",
author = "Grazia Galli and Sandra Nuti and Simona Tavarini and Luisa Galli-Stampino and {De Lalla}, Claudia and Giulia Casorati and Paolo Dellabona and Sergio Abrignani",
year = "2003",
month = "4",
day = "21",
doi = "10.1084/jem.20021616",
language = "English",
volume = "197",
pages = "1051--1057",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "8",

}

TY - JOUR

T1 - CD1d-restricted help to B cells by human invariant natural killer T lymphocytes

AU - Galli, Grazia

AU - Nuti, Sandra

AU - Tavarini, Simona

AU - Galli-Stampino, Luisa

AU - De Lalla, Claudia

AU - Casorati, Giulia

AU - Dellabona, Paolo

AU - Abrignani, Sergio

PY - 2003/4/21

Y1 - 2003/4/21

N2 - Invariant natural killer T (NKT) cells are a highly conserved subset of T lymphocytes expressing a semi-invariant T cell receptor (TCR), which is restricted to CDld and specific for the glycosphingolipid antigen α-galactosylceramide. Their ability to secrete a variety of cytokines, which in turn modulate the activation of cells of both innate and acquired immune responses, suggests that invariant NKT cells exert a regulatory role mainly via indirect mechanisms. A relevant question is whether invariant NKT cells can directly help B cells. We document here that human invariant NKT cells are as efficient as conventional CD4+ Th0 lymphocytes in promoting proliferation of autologous memory and naive B lymphocytes in vitro, and in inducing immunoglobulin production. Help to B cells by invariant NKT cells is CD1d-dependent and delivered also in the absence of α-galactosylceramide, suggesting that NKT cells recognize an endogenous ligand presented by CD1d on B cells. The two major subsets of invariant NKT cells, CD4+ and double negative (CD4-CD8-), express comparable levels of CD40 ligand and cytokines, but differ in helper functions. Indeed, both subsets induce similar levels of B cell proliferation, whereas CD4+ NKT cells induce higher levels of immunoglobulin production. These results suggest a direct role for invariant NKT cells in regulating B lymphocyte proliferation and effector functions.

AB - Invariant natural killer T (NKT) cells are a highly conserved subset of T lymphocytes expressing a semi-invariant T cell receptor (TCR), which is restricted to CDld and specific for the glycosphingolipid antigen α-galactosylceramide. Their ability to secrete a variety of cytokines, which in turn modulate the activation of cells of both innate and acquired immune responses, suggests that invariant NKT cells exert a regulatory role mainly via indirect mechanisms. A relevant question is whether invariant NKT cells can directly help B cells. We document here that human invariant NKT cells are as efficient as conventional CD4+ Th0 lymphocytes in promoting proliferation of autologous memory and naive B lymphocytes in vitro, and in inducing immunoglobulin production. Help to B cells by invariant NKT cells is CD1d-dependent and delivered also in the absence of α-galactosylceramide, suggesting that NKT cells recognize an endogenous ligand presented by CD1d on B cells. The two major subsets of invariant NKT cells, CD4+ and double negative (CD4-CD8-), express comparable levels of CD40 ligand and cytokines, but differ in helper functions. Indeed, both subsets induce similar levels of B cell proliferation, whereas CD4+ NKT cells induce higher levels of immunoglobulin production. These results suggest a direct role for invariant NKT cells in regulating B lymphocyte proliferation and effector functions.

KW - α-galactosylceramide

KW - Antibodies

KW - Autoreactivity

KW - Cytokine

KW - Helper assay

UR - http://www.scopus.com/inward/record.url?scp=0037460057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037460057&partnerID=8YFLogxK

U2 - 10.1084/jem.20021616

DO - 10.1084/jem.20021616

M3 - Article

C2 - 12695492

AN - SCOPUS:0037460057

VL - 197

SP - 1051

EP - 1057

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 8

ER -