CD27 distinguishes two phases in bone marrow infiltration of splenic marginal zone lymphoma

V. Franco, A. M. Florena, S. Ascani, M. Paulli, M. Salvato, S. A. Pileri

Research output: Contribution to journalArticle

Abstract

Aims: To investigate CD27 expression in splenic marginal zone lymphoma (SMZL), an indolent low-grade B-cell lymphoma with constant involvement of the bone marrow, especially with an intrasinusoidal pattern. It is not clear if the neoplastic clone is composed of virgin or somatically mutated B cells. CD27 is reported to be a hallmark of memory B cells. Methods and results: We evaluated 64 bone marrow biopsy specimens (BMBs) from 36 patients with SMZL for the expression of CD27. For comparison, splenectomy specimens of patients with traumatic splenic rupture or with SMZL were used. All BMBs showed lymphomatous infiltration. When located in the marrow sinusoids, neoplastic cells were CD27- in all cases and therefore corresponded to naive B cells. In nodular/interstitial infiltration, the cells were CD27+ and therefore corresponded to memory B cells. No difference in immunohistochemical expression of B and T antibodies was found between intrasinusoidal and interstitial/nodular infiltration. CD27 was constantly expressed in the splenic marginal zone of normal spleen, surgically removed for trauma, and in seven out of 10 spleens with SMZL. Conclusion: We propose the existence of two different phases of neoplastic progression with, first, expansion of a virgin B clone in the bone marrow and, following exposure to antigen, a re-colonization of the bone marrow.

Original languageEnglish
Pages (from-to)381-386
Number of pages6
JournalHistopathology
Volume44
Issue number4
DOIs
Publication statusPublished - Apr 2004

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Lymphoma
Bone Marrow
B-Lymphocytes
Spleen
Clone Cells
Splenic Rupture
B-Cell Lymphoma
Splenectomy
Non-Hodgkin's Lymphoma
Biopsy
Antigens
Antibodies
Wounds and Injuries

Keywords

  • Bone marrow
  • CD27
  • Intrasinusoidal infiltration
  • Naive and memory B cells
  • Splenic marginal zone lymphoma

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Cell Biology

Cite this

CD27 distinguishes two phases in bone marrow infiltration of splenic marginal zone lymphoma. / Franco, V.; Florena, A. M.; Ascani, S.; Paulli, M.; Salvato, M.; Pileri, S. A.

In: Histopathology, Vol. 44, No. 4, 04.2004, p. 381-386.

Research output: Contribution to journalArticle

Franco, V. ; Florena, A. M. ; Ascani, S. ; Paulli, M. ; Salvato, M. ; Pileri, S. A. / CD27 distinguishes two phases in bone marrow infiltration of splenic marginal zone lymphoma. In: Histopathology. 2004 ; Vol. 44, No. 4. pp. 381-386.
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AU - Salvato, M.

AU - Pileri, S. A.

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AB - Aims: To investigate CD27 expression in splenic marginal zone lymphoma (SMZL), an indolent low-grade B-cell lymphoma with constant involvement of the bone marrow, especially with an intrasinusoidal pattern. It is not clear if the neoplastic clone is composed of virgin or somatically mutated B cells. CD27 is reported to be a hallmark of memory B cells. Methods and results: We evaluated 64 bone marrow biopsy specimens (BMBs) from 36 patients with SMZL for the expression of CD27. For comparison, splenectomy specimens of patients with traumatic splenic rupture or with SMZL were used. All BMBs showed lymphomatous infiltration. When located in the marrow sinusoids, neoplastic cells were CD27- in all cases and therefore corresponded to naive B cells. In nodular/interstitial infiltration, the cells were CD27+ and therefore corresponded to memory B cells. No difference in immunohistochemical expression of B and T antibodies was found between intrasinusoidal and interstitial/nodular infiltration. CD27 was constantly expressed in the splenic marginal zone of normal spleen, surgically removed for trauma, and in seven out of 10 spleens with SMZL. Conclusion: We propose the existence of two different phases of neoplastic progression with, first, expansion of a virgin B clone in the bone marrow and, following exposure to antigen, a re-colonization of the bone marrow.

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