CD3-negative lymphokine-activated cytotoxic cells express the CD3ε gene

R. Biassoni, S. Ferrini, I. Prigione, A. Moretta, E. O. Long

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Abstract

The expression of genes encoding different polypeptide chains of the TCR-CD3 complex was analyzed in a panel of cloned MHC-unrestricted cytotoxic cells. The clones were derived from CD3+ and CD3- human PBL. After expansion in rIL-2, all clones were able to lyse the NK-sensitive target cell line K562. In contrast, Lysis of fresh tumor cells was achieved almost exclusively by CD3- clones. To test whether a known TCR-CD3 complex may be involved in MHC-unrestricted cytotoxicity, total RNA from nine CD3+ and 11 CD3- clones was isolated and hybridized with DNA probes for the TCRα-, β, and γ-chains and for the CD3γ-, δ-, and ε-chains. TCRγ transcripts were present at high levels in CD3+CD4-CD8- clones but were undetectable in all CD3- clones. Lysis of fresh tumor cells is an activity which can be independent of the TCRαβ and TCRγ complexes because the CD3- clones did not express the TCR genes. Interestingly, all CD3- clones expressed CD3ε transcripts, but not CD3γ- or δ-transcripts. CD3- lymphokine-activated cytotoxic cells may therefore be derived from immature T cells which do yet express a complete CD3 complex. The CD3ε chain, if expressed in CD3- cells in association with other molecules, could be involved in the activation and lytic function of these MHC-unrestricted cytotoxic cells.

Original languageEnglish
Pages (from-to)1685-1689
Number of pages5
JournalJournal of Immunology
Volume140
Issue number5
Publication statusPublished - 1988

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Lymphokines
Clone Cells
T-Cell Antigen Receptor-CD3 Complex
Genes
CD3 Antigens
DNA Probes
Neoplasms
RNA
T-Lymphocytes
Gene Expression
Cell Line
Peptides

ASJC Scopus subject areas

  • Immunology

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CD3-negative lymphokine-activated cytotoxic cells express the CD3ε gene. / Biassoni, R.; Ferrini, S.; Prigione, I.; Moretta, A.; Long, E. O.

In: Journal of Immunology, Vol. 140, No. 5, 1988, p. 1685-1689.

Research output: Contribution to journalArticle

Biassoni, R, Ferrini, S, Prigione, I, Moretta, A & Long, EO 1988, 'CD3-negative lymphokine-activated cytotoxic cells express the CD3ε gene', Journal of Immunology, vol. 140, no. 5, pp. 1685-1689.
Biassoni, R. ; Ferrini, S. ; Prigione, I. ; Moretta, A. ; Long, E. O. / CD3-negative lymphokine-activated cytotoxic cells express the CD3ε gene. In: Journal of Immunology. 1988 ; Vol. 140, No. 5. pp. 1685-1689.
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