CD344 cells mobilized by cyclophosphamide plus granulocyte colony-stimulating factor are functionally different from CD34+ cells mobilized by granulocyte colony-stimulating factor alone. C. Cesana

C. Carlo-Stella, L. Manyoni, E. Reyazzi, D. Garau, C. Almici, C. Caramatti, R. Giachetri, V. Rizzoli

Research output: Contribution to journalArticle

Abstract

Cyclophosphamide (CY) followed by granulocyte colony-stimulating factor (G-CSF) and G-CSF alone are the most commonly used PBPC mobilization schedules. To investigate whether the use of different mobilization regimens could result in functional differences of CD34+ cells, we analyzed nucleated cells (NC), CD34+ cells, colonyforming cells (CFC) and long-term culture initiating-cells (LTC-IC) in 52 leukaphcrcses from 26 patients with lymphoid malignances, mobilized either by CY+G-CSF (n=16) or G-CSF alone (n=10). Thirtyfour aphérèses from the CY+G-CSF group and 18 aphérèses from the G-CSF group were investigated. The mean (±SEM) number of NC was significantly lower in the CY+G-CSF products than in the G-CSF products (1.24±0.17xlO' vs 3.2±0.54xl(T, P<.0001). The mean (± SEM) incidence of CD34+ cells was significantly higher in the CY+GCSF products than in the G-CSF products (2.9±0.6 % vs 0.9±0.2%, P<.0018). However, the mean (± SEM) numbers of CD34+ cells mobilized per apheresis by CY+G-CSF and G-CSF were not significantly different (2.76±0.6x 10'vs 2.53±0.4xlO', PS .7). Similarly, no significant difference was detected by comparing the numbers (mean ± SEM) of CFC collected with the CY+G-CSF and the G-CSF schedule (10±2xl07 vs 4.6±lxl07, PS .09). Interestingly, CY+G-CSF mobilized CD34+ cells had a significantly higher plating efficiency (mean ± SEM) than G-CSF mobilized CD34+ cells (25.5±2.9% vs 10.8±1.9%, PS .0006). The mean (± SEM) number of LTC-IC was significantly higher in the CY+G-CSF products than in the G-CSF products (6.3±lxl06 vs 3.3±0.3xl06, P<.05). No other factor than the mobilizing regimen was found to significantly influence the yield of collected LTC-IC. In conclusion, our data show that CD34 cells mobilized by CY+G-CSF have higher clonogenic activity and primitive progenitor cell content than CD34+ cells mobilized by G-CSF. As mobilized PBPC containing large number of progenitors lead to safer transplantation, this issue may have implications in autografting.

Original languageEnglish
Pages (from-to)805
Number of pages1
JournalExperimental Hematology
Volume25
Issue number8
Publication statusPublished - 1997

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Granulocyte Colony-Stimulating Factor
Cyclophosphamide
Cell Culture Techniques
Appointments and Schedules
Cell Count
Blood Component Removal
Autologous Transplantation

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

CD344 cells mobilized by cyclophosphamide plus granulocyte colony-stimulating factor are functionally different from CD34+ cells mobilized by granulocyte colony-stimulating factor alone. C. Cesana. / Carlo-Stella, C.; Manyoni, L.; Reyazzi, E.; Garau, D.; Almici, C.; Caramatti, C.; Giachetri, R.; Rizzoli, V.

In: Experimental Hematology, Vol. 25, No. 8, 1997, p. 805.

Research output: Contribution to journalArticle

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title = "CD344 cells mobilized by cyclophosphamide plus granulocyte colony-stimulating factor are functionally different from CD34+ cells mobilized by granulocyte colony-stimulating factor alone. C. Cesana",
abstract = "Cyclophosphamide (CY) followed by granulocyte colony-stimulating factor (G-CSF) and G-CSF alone are the most commonly used PBPC mobilization schedules. To investigate whether the use of different mobilization regimens could result in functional differences of CD34+ cells, we analyzed nucleated cells (NC), CD34+ cells, colonyforming cells (CFC) and long-term culture initiating-cells (LTC-IC) in 52 leukaphcrcses from 26 patients with lymphoid malignances, mobilized either by CY+G-CSF (n=16) or G-CSF alone (n=10). Thirtyfour aph{\'e}r{\`e}ses from the CY+G-CSF group and 18 aph{\'e}r{\`e}ses from the G-CSF group were investigated. The mean (±SEM) number of NC was significantly lower in the CY+G-CSF products than in the G-CSF products (1.24±0.17xlO' vs 3.2±0.54xl(T, P<.0001). The mean (± SEM) incidence of CD34+ cells was significantly higher in the CY+GCSF products than in the G-CSF products (2.9±0.6 {\%} vs 0.9±0.2{\%}, P<.0018). However, the mean (± SEM) numbers of CD34+ cells mobilized per apheresis by CY+G-CSF and G-CSF were not significantly different (2.76±0.6x 10'vs 2.53±0.4xlO', PS .7). Similarly, no significant difference was detected by comparing the numbers (mean ± SEM) of CFC collected with the CY+G-CSF and the G-CSF schedule (10±2xl07 vs 4.6±lxl07, PS .09). Interestingly, CY+G-CSF mobilized CD34+ cells had a significantly higher plating efficiency (mean ± SEM) than G-CSF mobilized CD34+ cells (25.5±2.9{\%} vs 10.8±1.9{\%}, PS .0006). The mean (± SEM) number of LTC-IC was significantly higher in the CY+G-CSF products than in the G-CSF products (6.3±lxl06 vs 3.3±0.3xl06, P<.05). No other factor than the mobilizing regimen was found to significantly influence the yield of collected LTC-IC. In conclusion, our data show that CD34 cells mobilized by CY+G-CSF have higher clonogenic activity and primitive progenitor cell content than CD34+ cells mobilized by G-CSF. As mobilized PBPC containing large number of progenitors lead to safer transplantation, this issue may have implications in autografting.",
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T1 - CD344 cells mobilized by cyclophosphamide plus granulocyte colony-stimulating factor are functionally different from CD34+ cells mobilized by granulocyte colony-stimulating factor alone. C. Cesana

AU - Carlo-Stella, C.

AU - Manyoni, L.

AU - Reyazzi, E.

AU - Garau, D.

AU - Almici, C.

AU - Caramatti, C.

AU - Giachetri, R.

AU - Rizzoli, V.

PY - 1997

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N2 - Cyclophosphamide (CY) followed by granulocyte colony-stimulating factor (G-CSF) and G-CSF alone are the most commonly used PBPC mobilization schedules. To investigate whether the use of different mobilization regimens could result in functional differences of CD34+ cells, we analyzed nucleated cells (NC), CD34+ cells, colonyforming cells (CFC) and long-term culture initiating-cells (LTC-IC) in 52 leukaphcrcses from 26 patients with lymphoid malignances, mobilized either by CY+G-CSF (n=16) or G-CSF alone (n=10). Thirtyfour aphérèses from the CY+G-CSF group and 18 aphérèses from the G-CSF group were investigated. The mean (±SEM) number of NC was significantly lower in the CY+G-CSF products than in the G-CSF products (1.24±0.17xlO' vs 3.2±0.54xl(T, P<.0001). The mean (± SEM) incidence of CD34+ cells was significantly higher in the CY+GCSF products than in the G-CSF products (2.9±0.6 % vs 0.9±0.2%, P<.0018). However, the mean (± SEM) numbers of CD34+ cells mobilized per apheresis by CY+G-CSF and G-CSF were not significantly different (2.76±0.6x 10'vs 2.53±0.4xlO', PS .7). Similarly, no significant difference was detected by comparing the numbers (mean ± SEM) of CFC collected with the CY+G-CSF and the G-CSF schedule (10±2xl07 vs 4.6±lxl07, PS .09). Interestingly, CY+G-CSF mobilized CD34+ cells had a significantly higher plating efficiency (mean ± SEM) than G-CSF mobilized CD34+ cells (25.5±2.9% vs 10.8±1.9%, PS .0006). The mean (± SEM) number of LTC-IC was significantly higher in the CY+G-CSF products than in the G-CSF products (6.3±lxl06 vs 3.3±0.3xl06, P<.05). No other factor than the mobilizing regimen was found to significantly influence the yield of collected LTC-IC. In conclusion, our data show that CD34 cells mobilized by CY+G-CSF have higher clonogenic activity and primitive progenitor cell content than CD34+ cells mobilized by G-CSF. As mobilized PBPC containing large number of progenitors lead to safer transplantation, this issue may have implications in autografting.

AB - Cyclophosphamide (CY) followed by granulocyte colony-stimulating factor (G-CSF) and G-CSF alone are the most commonly used PBPC mobilization schedules. To investigate whether the use of different mobilization regimens could result in functional differences of CD34+ cells, we analyzed nucleated cells (NC), CD34+ cells, colonyforming cells (CFC) and long-term culture initiating-cells (LTC-IC) in 52 leukaphcrcses from 26 patients with lymphoid malignances, mobilized either by CY+G-CSF (n=16) or G-CSF alone (n=10). Thirtyfour aphérèses from the CY+G-CSF group and 18 aphérèses from the G-CSF group were investigated. The mean (±SEM) number of NC was significantly lower in the CY+G-CSF products than in the G-CSF products (1.24±0.17xlO' vs 3.2±0.54xl(T, P<.0001). The mean (± SEM) incidence of CD34+ cells was significantly higher in the CY+GCSF products than in the G-CSF products (2.9±0.6 % vs 0.9±0.2%, P<.0018). However, the mean (± SEM) numbers of CD34+ cells mobilized per apheresis by CY+G-CSF and G-CSF were not significantly different (2.76±0.6x 10'vs 2.53±0.4xlO', PS .7). Similarly, no significant difference was detected by comparing the numbers (mean ± SEM) of CFC collected with the CY+G-CSF and the G-CSF schedule (10±2xl07 vs 4.6±lxl07, PS .09). Interestingly, CY+G-CSF mobilized CD34+ cells had a significantly higher plating efficiency (mean ± SEM) than G-CSF mobilized CD34+ cells (25.5±2.9% vs 10.8±1.9%, PS .0006). The mean (± SEM) number of LTC-IC was significantly higher in the CY+G-CSF products than in the G-CSF products (6.3±lxl06 vs 3.3±0.3xl06, P<.05). No other factor than the mobilizing regimen was found to significantly influence the yield of collected LTC-IC. In conclusion, our data show that CD34 cells mobilized by CY+G-CSF have higher clonogenic activity and primitive progenitor cell content than CD34+ cells mobilized by G-CSF. As mobilized PBPC containing large number of progenitors lead to safer transplantation, this issue may have implications in autografting.

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