CD4 engagement by HIV-1 in TF-1 hematopoietic progenitor cells increases protein kinase C activity and reduces intracellular Ca2+ levels.

D. Gibellini, G. Zauli, M. C. Re, G. Furlini, S. Lolli, P. Borgatti, S. Capitani, M. La Placa

Research output: Contribution to journalArticlepeer-review

Abstract

Starting from our previous observations that the HIV-1-mediated engagement of CD4 induced apoptotic death of TF-1 hematopoietic progenitor cells, in this study we evaluated PKC activity and intracellular Ca2+ levels in TF-1 cells treated with viable and heat-inactivated HIV-1 (strain IIIB) or anti-CD4 Leu3a monoclonal antibody (mAb). Both viable and heat-inactivated HIV-1 or anti-CD4 mAb, but not anti-human cytomegalovirus (HCMV) 66kD protein or anti-CD8 mAb induced a rapid (5-10 min) increase in PKC activity under both serum-containing and serum-free conditions. The same treatment also induced both a transient and a long-lasting (48 hours) decrease (p <0.05) in intracellular Ca2+ levels in serum-containing cultures. We propose that the observed changes in PKC activity and intracellular Ca2+ levels might be involved in the HIV-1 mediated apoptosis of hematopoietic progenitor cells.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalNew Microbiologica
Volume17
Issue number2
Publication statusPublished - Apr 1994

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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