CD4 epitope masking by gp120/aNTI-gp120 antibody complexes: A potential mechanism for CD4+ cell function down-regulation in AIDS patients

Alberto Amadori, Giustina De Silvestro, Rita Zamarchi, Maria Luisa Veronese, Maria Rosaria Mazza, Giampietro Schiavo, Marina Panozzo, Anita De Rossi, Lucia Ometto, Jan Mous, Andrea Barelli, Alfredo Borri, Leopoldo Salmaso, Luigi Chieco-Bianchi

Research output: Contribution to journalArticle

Abstract

The in vitro suppressive effect of gp120 and gp120/anti-gp120 antibody is well known but not yet proven to operate in vivo. We report findings consistent with the presence of gp120/anti-gp120 antibody complexes on CD4+ lymphocytes from HIV-infected patients with advanced disease. PBMC from most AIDS patients showed selective masking of the CD4 epitope associated with the gp120 binding site; immunoprecipitation of PBMC with anti-CD4 mAb disclosed high amounts of IgG bound to CD4 receptors. Antibodies against HIV env proteins, but not other HIV products or CD4 Ag, were detected in purified CD4+ cell culture supernatants; in vitro culture was associated with normalization of both CD4 expression in PBMC and the lymphocyte proliferative response to anti-CD3. gp120 presence could not be directly demonstrated, but findings strongly suggested that CD4+ lymphocytes from most HIV-infected patients with advanced disease were covered with gp120/anti-gp120 antibody complexes, which are responsible for down-regulation of surface CD4 expression as well as functional lymphocyte impairment; this event may represent an important mechanism in the pathogenesis of HIV-associated immunodeficiency.

Original languageEnglish
Pages (from-to)2709-2716
Number of pages8
JournalJournal of Immunology
Volume148
Issue number9
Publication statusPublished - May 1 1992

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'CD4 epitope masking by gp120/aNTI-gp120 antibody complexes: A potential mechanism for CD4+ cell function down-regulation in AIDS patients'. Together they form a unique fingerprint.

  • Cite this

    Amadori, A., De Silvestro, G., Zamarchi, R., Veronese, M. L., Mazza, M. R., Schiavo, G., Panozzo, M., De Rossi, A., Ometto, L., Mous, J., Barelli, A., Borri, A., Salmaso, L., & Chieco-Bianchi, L. (1992). CD4 epitope masking by gp120/aNTI-gp120 antibody complexes: A potential mechanism for CD4+ cell function down-regulation in AIDS patients. Journal of Immunology, 148(9), 2709-2716.