CD4 lymphocyte enumeration: Comparison between flow cytometry and enzyme immunoassay

P. Cordiali Fei, M. Solmone, P. Vanacore, A. Giglio, C. Bonifati, M. Carducci, A. Mussi, F. Chiarotti, F. Ameglio

Research output: Contribution to journalArticle

Abstract

Assessment of the CD4 lymphocyte number, currently performed by flow cytometry, is one of the main laboratory tests for establishing progression to acquired immunodeficiency syndrome (AIDS). An enzyme immunoassay has recently been commercialized which can be very useful for counting CD4 cells in laboratories where flow cytometers are not available. In the present study, a comparative evaluation of CD4 positive lymphocytes with flow cytometry and the enzyme assay was made in healthy subjects (N = 30, R = 0.88, P <0.0001), human immunodeficiency virus (HIV)-infected individuals (N = 80, R = 0.91, P <0.0001), and patients with autoimmune diseases (N = 28, R = 0.82, P <0.001) or psoriasis (N = 18, R = 0.76, P = 0.01). A correlation between the two methodologies was not found in psoriatic patients under treatment with cyclosporin A (N = 7, R = 0.05, not significant). Some differences could be found at low CD4 lymphocyte levels since the influence of CD4 antigen eluted from monocytes or soluble CD4 in the whole blood sample could cause overestimation of CD4 cell numbers by the enzyme assay.

Original languageEnglish
Pages (from-to)70-74
Number of pages5
JournalCytometry
Volume22
Issue number1
DOIs
Publication statusPublished - 1995

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Keywords

  • CD4 evaluation
  • Cyclosporin A
  • HIV infection
  • Psoriasis

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Cordiali Fei, P., Solmone, M., Vanacore, P., Giglio, A., Bonifati, C., Carducci, M., Mussi, A., Chiarotti, F., & Ameglio, F. (1995). CD4 lymphocyte enumeration: Comparison between flow cytometry and enzyme immunoassay. Cytometry, 22(1), 70-74. https://doi.org/10.1002/cyto.990220113