Abstract
A role for CD4 +CD25 + regulatory T cells (Tregs) in the control of allergic diseases has been postulated. We developed a mouse model in which anaphylaxis is induced in SJL mice by immunization and challenge with the fragment of self myelin proteolipid protein (PLP) 139-151 that is not expressed in the thymus, but not with fragment 178-191 of the same protein, that is expressed in the thymus. In this study, we show that resistance to anaphylaxis is associated with naturally occurring CD4 +CD25 + Tregs specific for the self peptide expressed in the thymus. These cells increase Foxp3 expression upon Ag stimulation and suppress peptide-induced proliferation of CD4 +CD25 - effector T cells. Depletion of Tregs with anti-CD25 in vivo significantly diminished resistance to anaphylaxis to PLP 178-191, suggesting an important role for CD4 +CD25 + Tregs in preventing the development of allergic responses to this thymus-expressed peptide. These data indicate that naturally occurring CD4 +CD25 + Tregs specific for a peptide expressed under physiological conditions in the thymus are able to suppress the development of a systemic allergic reaction to self.
| Original language | English |
|---|---|
| Pages (from-to) | 4433-4440 |
| Number of pages | 8 |
| Journal | Journal of Immunology |
| Volume | 180 |
| Issue number | 7 |
| Publication status | Published - Apr 1 2008 |
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ASJC Scopus subject areas
- Immunology
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CD4 +CD25 +regulatory T cells specific for a thymus-expressed antigen prevent the development of anaphylaxis to self. / Scabeni, Stefano; Lapilla, Marilena; Musio, Silvia; Gallo, Barbara; Ciusani, Emilio; Steinman, Lawrence; Mantegazza, Renato; Pedotti, Rosetta.
In: Journal of Immunology, Vol. 180, No. 7, 01.04.2008, p. 4433-4440.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CD4 +CD25 +regulatory T cells specific for a thymus-expressed antigen prevent the development of anaphylaxis to self
AU - Scabeni, Stefano
AU - Lapilla, Marilena
AU - Musio, Silvia
AU - Gallo, Barbara
AU - Ciusani, Emilio
AU - Steinman, Lawrence
AU - Mantegazza, Renato
AU - Pedotti, Rosetta
PY - 2008/4/1
Y1 - 2008/4/1
N2 - A role for CD4 +CD25 + regulatory T cells (Tregs) in the control of allergic diseases has been postulated. We developed a mouse model in which anaphylaxis is induced in SJL mice by immunization and challenge with the fragment of self myelin proteolipid protein (PLP) 139-151 that is not expressed in the thymus, but not with fragment 178-191 of the same protein, that is expressed in the thymus. In this study, we show that resistance to anaphylaxis is associated with naturally occurring CD4 +CD25 + Tregs specific for the self peptide expressed in the thymus. These cells increase Foxp3 expression upon Ag stimulation and suppress peptide-induced proliferation of CD4 +CD25 - effector T cells. Depletion of Tregs with anti-CD25 in vivo significantly diminished resistance to anaphylaxis to PLP 178-191, suggesting an important role for CD4 +CD25 + Tregs in preventing the development of allergic responses to this thymus-expressed peptide. These data indicate that naturally occurring CD4 +CD25 + Tregs specific for a peptide expressed under physiological conditions in the thymus are able to suppress the development of a systemic allergic reaction to self.
AB - A role for CD4 +CD25 + regulatory T cells (Tregs) in the control of allergic diseases has been postulated. We developed a mouse model in which anaphylaxis is induced in SJL mice by immunization and challenge with the fragment of self myelin proteolipid protein (PLP) 139-151 that is not expressed in the thymus, but not with fragment 178-191 of the same protein, that is expressed in the thymus. In this study, we show that resistance to anaphylaxis is associated with naturally occurring CD4 +CD25 + Tregs specific for the self peptide expressed in the thymus. These cells increase Foxp3 expression upon Ag stimulation and suppress peptide-induced proliferation of CD4 +CD25 - effector T cells. Depletion of Tregs with anti-CD25 in vivo significantly diminished resistance to anaphylaxis to PLP 178-191, suggesting an important role for CD4 +CD25 + Tregs in preventing the development of allergic responses to this thymus-expressed peptide. These data indicate that naturally occurring CD4 +CD25 + Tregs specific for a peptide expressed under physiological conditions in the thymus are able to suppress the development of a systemic allergic reaction to self.
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UR - http://www.scopus.com/inward/citedby.url?scp=44449159981&partnerID=8YFLogxK
M3 - Article
C2 - 18354164
AN - SCOPUS:44449159981
VL - 180
SP - 4433
EP - 4440
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 7
ER -