CD4 +CD25 +regulatory T cells specific for a thymus-expressed antigen prevent the development of anaphylaxis to self

Stefano Scabeni, Marilena Lapilla, Silvia Musio, Barbara Gallo, Emilio Ciusani, Lawrence Steinman, Renato Mantegazza, Rosetta Pedotti

Research output: Contribution to journalArticle

Abstract

A role for CD4 +CD25 + regulatory T cells (Tregs) in the control of allergic diseases has been postulated. We developed a mouse model in which anaphylaxis is induced in SJL mice by immunization and challenge with the fragment of self myelin proteolipid protein (PLP) 139-151 that is not expressed in the thymus, but not with fragment 178-191 of the same protein, that is expressed in the thymus. In this study, we show that resistance to anaphylaxis is associated with naturally occurring CD4 +CD25 + Tregs specific for the self peptide expressed in the thymus. These cells increase Foxp3 expression upon Ag stimulation and suppress peptide-induced proliferation of CD4 +CD25 - effector T cells. Depletion of Tregs with anti-CD25 in vivo significantly diminished resistance to anaphylaxis to PLP 178-191, suggesting an important role for CD4 +CD25 + Tregs in preventing the development of allergic responses to this thymus-expressed peptide. These data indicate that naturally occurring CD4 +CD25 + Tregs specific for a peptide expressed under physiological conditions in the thymus are able to suppress the development of a systemic allergic reaction to self.

Original languageEnglish
Pages (from-to)4433-4440
Number of pages8
JournalJournal of Immunology
Volume180
Issue number7
Publication statusPublished - Apr 1 2008

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Anaphylaxis
Regulatory T-Lymphocytes
Thymus Gland
Antigens
Peptides
Immunization
Hypersensitivity
T-Lymphocytes
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Scabeni, S., Lapilla, M., Musio, S., Gallo, B., Ciusani, E., Steinman, L., ... Pedotti, R. (2008). CD4 +CD25 +regulatory T cells specific for a thymus-expressed antigen prevent the development of anaphylaxis to self. Journal of Immunology, 180(7), 4433-4440.

CD4 +CD25 +regulatory T cells specific for a thymus-expressed antigen prevent the development of anaphylaxis to self. / Scabeni, Stefano; Lapilla, Marilena; Musio, Silvia; Gallo, Barbara; Ciusani, Emilio; Steinman, Lawrence; Mantegazza, Renato; Pedotti, Rosetta.

In: Journal of Immunology, Vol. 180, No. 7, 01.04.2008, p. 4433-4440.

Research output: Contribution to journalArticle

Scabeni, S, Lapilla, M, Musio, S, Gallo, B, Ciusani, E, Steinman, L, Mantegazza, R & Pedotti, R 2008, 'CD4 +CD25 +regulatory T cells specific for a thymus-expressed antigen prevent the development of anaphylaxis to self', Journal of Immunology, vol. 180, no. 7, pp. 4433-4440.
Scabeni, Stefano ; Lapilla, Marilena ; Musio, Silvia ; Gallo, Barbara ; Ciusani, Emilio ; Steinman, Lawrence ; Mantegazza, Renato ; Pedotti, Rosetta. / CD4 +CD25 +regulatory T cells specific for a thymus-expressed antigen prevent the development of anaphylaxis to self. In: Journal of Immunology. 2008 ; Vol. 180, No. 7. pp. 4433-4440.
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