CD4 T lymphocyte autophagy is upregulated in the salivary glands of primary Sjögren's syndrome patients and correlates with focus score and disease activity

Cristiano Alessandri, Francesco Ciccia, Roberta Priori, Elisa Astorri, Giuliana Guggino, Riccardo Alessandro, Aroldo Rizzo, Fabrizio Conti, Antonina Minniti, Cristiana Barbati, Marta Vomero, Monica Pendolino, Annacarla Finucci, Elena Ortona, Tania Colasanti, Marina Pierdominici, Walter Malorni, Giovanni Triolo, Guido Valesini

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Primary Sjögren's syndrome (pSS) is a common chronic autoimmune disease characterized by lymphocytic infiltration of exocrine glands and peripheral lymphocyte perturbation. In the current study, we aimed to investigate the possible pathogenic implication of autophagy in T lymphocytes in patients with pSS. Methods: Thirty consecutive pSS patients were recruited together with 20 patients affected by sicca syndrome and/or chronic sialoadenitis and 30 healthy controls. Disease activity and damage were evaluated according to SS disease activity index, EULAR SS disease activity index, and SS disease damage index. T lymphocytes were analyzed for the expression of autophagy-specific markers by biochemical, molecular, and histological assays in peripheral blood and labial gland biopsies. Serum interleukin (IL)-23 and IL-21 levels were quantified by enzyme-linked immunosorbent assay. Results: Our study provides evidence for the first time that autophagy is upregulated in CD4+ T lymphocyte salivary glands from pSS patients. Furthermore, a statistically significant correlation was detected between lymphocyte autophagy levels, disease activity, and damage indexes. We also found a positive correlation between autophagy enhancement and the increased salivary gland expression of IL-21 and IL-23, providing a further link between innate and adaptive immune responses in pSS. Conclusions: These findings suggest that CD4+ T lymphocyte autophagy could play a key role in pSS pathogenesis. Additionally, our data highlight the potential exploitation of T cell autophagy as a biomarker of disease activity and provide new ground to verify the therapeutic implications of autophagy as an innovative drug target in pSS.

Original languageEnglish
Article number178
JournalArthritis Research and Therapy
Volume19
Issue number1
DOIs
Publication statusPublished - Jul 25 2017

Fingerprint

Autophagy
Salivary Glands
T-Lymphocytes
Interleukin-23
Biomarkers
Exocrine Glands
Lymphocytes
Sialadenitis
Sjogren's Syndrome
Adaptive Immunity
Lip
Innate Immunity
Autoimmune Diseases
Chronic Disease
Enzyme-Linked Immunosorbent Assay
Biopsy
Serum
Pharmaceutical Preparations

Keywords

  • Autophagy
  • Cytokines
  • Lymphocytes
  • Sjögren syndrome

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

Cite this

CD4 T lymphocyte autophagy is upregulated in the salivary glands of primary Sjögren's syndrome patients and correlates with focus score and disease activity. / Alessandri, Cristiano; Ciccia, Francesco; Priori, Roberta; Astorri, Elisa; Guggino, Giuliana; Alessandro, Riccardo; Rizzo, Aroldo; Conti, Fabrizio; Minniti, Antonina; Barbati, Cristiana; Vomero, Marta; Pendolino, Monica; Finucci, Annacarla; Ortona, Elena; Colasanti, Tania; Pierdominici, Marina; Malorni, Walter; Triolo, Giovanni; Valesini, Guido.

In: Arthritis Research and Therapy, Vol. 19, No. 1, 178, 25.07.2017.

Research output: Contribution to journalArticle

Alessandri, C, Ciccia, F, Priori, R, Astorri, E, Guggino, G, Alessandro, R, Rizzo, A, Conti, F, Minniti, A, Barbati, C, Vomero, M, Pendolino, M, Finucci, A, Ortona, E, Colasanti, T, Pierdominici, M, Malorni, W, Triolo, G & Valesini, G 2017, 'CD4 T lymphocyte autophagy is upregulated in the salivary glands of primary Sjögren's syndrome patients and correlates with focus score and disease activity', Arthritis Research and Therapy, vol. 19, no. 1, 178. https://doi.org/10.1186/s13075-017-1385-y
Alessandri, Cristiano ; Ciccia, Francesco ; Priori, Roberta ; Astorri, Elisa ; Guggino, Giuliana ; Alessandro, Riccardo ; Rizzo, Aroldo ; Conti, Fabrizio ; Minniti, Antonina ; Barbati, Cristiana ; Vomero, Marta ; Pendolino, Monica ; Finucci, Annacarla ; Ortona, Elena ; Colasanti, Tania ; Pierdominici, Marina ; Malorni, Walter ; Triolo, Giovanni ; Valesini, Guido. / CD4 T lymphocyte autophagy is upregulated in the salivary glands of primary Sjögren's syndrome patients and correlates with focus score and disease activity. In: Arthritis Research and Therapy. 2017 ; Vol. 19, No. 1.
@article{c345a240687e4698aa89dd1af15c7dbb,
title = "CD4 T lymphocyte autophagy is upregulated in the salivary glands of primary Sj{\"o}gren's syndrome patients and correlates with focus score and disease activity",
abstract = "Background: Primary Sj{\"o}gren's syndrome (pSS) is a common chronic autoimmune disease characterized by lymphocytic infiltration of exocrine glands and peripheral lymphocyte perturbation. In the current study, we aimed to investigate the possible pathogenic implication of autophagy in T lymphocytes in patients with pSS. Methods: Thirty consecutive pSS patients were recruited together with 20 patients affected by sicca syndrome and/or chronic sialoadenitis and 30 healthy controls. Disease activity and damage were evaluated according to SS disease activity index, EULAR SS disease activity index, and SS disease damage index. T lymphocytes were analyzed for the expression of autophagy-specific markers by biochemical, molecular, and histological assays in peripheral blood and labial gland biopsies. Serum interleukin (IL)-23 and IL-21 levels were quantified by enzyme-linked immunosorbent assay. Results: Our study provides evidence for the first time that autophagy is upregulated in CD4+ T lymphocyte salivary glands from pSS patients. Furthermore, a statistically significant correlation was detected between lymphocyte autophagy levels, disease activity, and damage indexes. We also found a positive correlation between autophagy enhancement and the increased salivary gland expression of IL-21 and IL-23, providing a further link between innate and adaptive immune responses in pSS. Conclusions: These findings suggest that CD4+ T lymphocyte autophagy could play a key role in pSS pathogenesis. Additionally, our data highlight the potential exploitation of T cell autophagy as a biomarker of disease activity and provide new ground to verify the therapeutic implications of autophagy as an innovative drug target in pSS.",
keywords = "Autophagy, Cytokines, Lymphocytes, Sj{\"o}gren syndrome",
author = "Cristiano Alessandri and Francesco Ciccia and Roberta Priori and Elisa Astorri and Giuliana Guggino and Riccardo Alessandro and Aroldo Rizzo and Fabrizio Conti and Antonina Minniti and Cristiana Barbati and Marta Vomero and Monica Pendolino and Annacarla Finucci and Elena Ortona and Tania Colasanti and Marina Pierdominici and Walter Malorni and Giovanni Triolo and Guido Valesini",
year = "2017",
month = "7",
day = "25",
doi = "10.1186/s13075-017-1385-y",
language = "English",
volume = "19",
journal = "Arthritis Research and Therapy",
issn = "1478-6354",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - CD4 T lymphocyte autophagy is upregulated in the salivary glands of primary Sjögren's syndrome patients and correlates with focus score and disease activity

AU - Alessandri, Cristiano

AU - Ciccia, Francesco

AU - Priori, Roberta

AU - Astorri, Elisa

AU - Guggino, Giuliana

AU - Alessandro, Riccardo

AU - Rizzo, Aroldo

AU - Conti, Fabrizio

AU - Minniti, Antonina

AU - Barbati, Cristiana

AU - Vomero, Marta

AU - Pendolino, Monica

AU - Finucci, Annacarla

AU - Ortona, Elena

AU - Colasanti, Tania

AU - Pierdominici, Marina

AU - Malorni, Walter

AU - Triolo, Giovanni

AU - Valesini, Guido

PY - 2017/7/25

Y1 - 2017/7/25

N2 - Background: Primary Sjögren's syndrome (pSS) is a common chronic autoimmune disease characterized by lymphocytic infiltration of exocrine glands and peripheral lymphocyte perturbation. In the current study, we aimed to investigate the possible pathogenic implication of autophagy in T lymphocytes in patients with pSS. Methods: Thirty consecutive pSS patients were recruited together with 20 patients affected by sicca syndrome and/or chronic sialoadenitis and 30 healthy controls. Disease activity and damage were evaluated according to SS disease activity index, EULAR SS disease activity index, and SS disease damage index. T lymphocytes were analyzed for the expression of autophagy-specific markers by biochemical, molecular, and histological assays in peripheral blood and labial gland biopsies. Serum interleukin (IL)-23 and IL-21 levels were quantified by enzyme-linked immunosorbent assay. Results: Our study provides evidence for the first time that autophagy is upregulated in CD4+ T lymphocyte salivary glands from pSS patients. Furthermore, a statistically significant correlation was detected between lymphocyte autophagy levels, disease activity, and damage indexes. We also found a positive correlation between autophagy enhancement and the increased salivary gland expression of IL-21 and IL-23, providing a further link between innate and adaptive immune responses in pSS. Conclusions: These findings suggest that CD4+ T lymphocyte autophagy could play a key role in pSS pathogenesis. Additionally, our data highlight the potential exploitation of T cell autophagy as a biomarker of disease activity and provide new ground to verify the therapeutic implications of autophagy as an innovative drug target in pSS.

AB - Background: Primary Sjögren's syndrome (pSS) is a common chronic autoimmune disease characterized by lymphocytic infiltration of exocrine glands and peripheral lymphocyte perturbation. In the current study, we aimed to investigate the possible pathogenic implication of autophagy in T lymphocytes in patients with pSS. Methods: Thirty consecutive pSS patients were recruited together with 20 patients affected by sicca syndrome and/or chronic sialoadenitis and 30 healthy controls. Disease activity and damage were evaluated according to SS disease activity index, EULAR SS disease activity index, and SS disease damage index. T lymphocytes were analyzed for the expression of autophagy-specific markers by biochemical, molecular, and histological assays in peripheral blood and labial gland biopsies. Serum interleukin (IL)-23 and IL-21 levels were quantified by enzyme-linked immunosorbent assay. Results: Our study provides evidence for the first time that autophagy is upregulated in CD4+ T lymphocyte salivary glands from pSS patients. Furthermore, a statistically significant correlation was detected between lymphocyte autophagy levels, disease activity, and damage indexes. We also found a positive correlation between autophagy enhancement and the increased salivary gland expression of IL-21 and IL-23, providing a further link between innate and adaptive immune responses in pSS. Conclusions: These findings suggest that CD4+ T lymphocyte autophagy could play a key role in pSS pathogenesis. Additionally, our data highlight the potential exploitation of T cell autophagy as a biomarker of disease activity and provide new ground to verify the therapeutic implications of autophagy as an innovative drug target in pSS.

KW - Autophagy

KW - Cytokines

KW - Lymphocytes

KW - Sjögren syndrome

UR - http://www.scopus.com/inward/record.url?scp=85026244679&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026244679&partnerID=8YFLogxK

U2 - 10.1186/s13075-017-1385-y

DO - 10.1186/s13075-017-1385-y

M3 - Article

VL - 19

JO - Arthritis Research and Therapy

JF - Arthritis Research and Therapy

SN - 1478-6354

IS - 1

M1 - 178

ER -