CD4+ Th0 cell clones, isolated from a metastatic lymph node of a melanoma patient, possess cytolytic function

Maria Adele Imro, Corrado Castagneto, Ornella Bosco, Paola Modena, Lorella Lanza, Francesco Puppo, Gilberto Filaci, Francesco Indiveri, Marco Scudeletti

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study T lymphocytes isolated from a metastatic lymph node (T-LNL) of a melanoma patient have been cloned. In the attempt to verify whether T-LNL may acquire in vitro functional activities in the absence of tumour-associated antigens, they were cloned utilizing allogeneic lymphocytes as feeder cells. Nineteen clones generated from T-LNL proved to be CD4+ and, among these, five were able to kill autologous and allogeneic human melanoma cells in HLA-class-II-restricted way. On the basis of their cytokine production, these CD4+ cytolytic T-LNL clones were shown to belong to the Th0 subset and three of them expersseed the Vβ17 chain of the T cell receptor. These results suggest the presence of melanomaspecific but functionally inactive lymphocytes with T cell receptor oligoclonality in the lymph node environment. These specific T cells may acquire in vitro the capacity to kill autologous and allogeneic tumours without any induction by autologous melanoma cells.

Original languageEnglish
Pages (from-to)210-216
Number of pages7
JournalCancer Immunology, Immunotherapy
Volume41
Issue number4
DOIs
Publication statusPublished - Jul 1995

Keywords

  • Cr release
  • Cytokines
  • Lymph node
  • Lymphocytes
  • Tumour

ASJC Scopus subject areas

  • Oncology
  • Immunology
  • Cancer Research

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