CD40 expressed on human melanoma cells mediates T cell co-stimulation and tumor cell growth

Giuseppe Pirozzi, Vincenza Lombari, Delia Zanzi, Franco Ionna, Maria Luisa Lombardi, Simona Errico, Giuseppina Ruggiero, Ciro Manzo

Research output: Contribution to journalArticle

Abstract

CD40 is a 50 kDa molecule, a member of the tumor necrosis factor/nerve growth factor receptor family. It is expressed on B cells, monocytes, dendritic cells and various malignant cells. While the critical relevance of this molecule in T cell-dependent B cell activation is already established, the biological role of CD40-CD154 interaction in non-hematopoietic cells is still unknown, Here we show that CD40 is functionally expressed on human melanoma-derived cell lines. No correlation between surface CD40 expression and the origin of the cell line, primary versus metastatic, was observed. Melanoma cells were shown to be able to co-stimulate TCR-triggered human T cells; moreover, because they do not express CD80 or CD86 co-stimulatory structures, the involvement of additional pathways have to be postulated. We have identified CD40 as one of the molecules involved in melanoma cell-mediated co-stimulation of anti-CD3-triggered human CD4+ T lymphocytes. In addition, a CD40-dependent pathway, able to enhance tumor cell proliferation at low serum concentrations, in vitro, has been shown to be functional in human melanoma cell lines.

Original languageEnglish
Pages (from-to)787-795
Number of pages9
JournalInternational Immunology
Volume12
Issue number6
Publication statusPublished - 2000

Fingerprint

Melanoma
T-Lymphocytes
Growth
Cell Line
Neoplasms
B-Lymphocytes
Nerve Growth Factor Receptor
Dendritic Cells
Monocytes
Tumor Necrosis Factor-alpha
Cell Proliferation
Serum

Keywords

  • CD40
  • Cell growth
  • Co-stimulation
  • Melanoma

ASJC Scopus subject areas

  • Immunology

Cite this

Pirozzi, G., Lombari, V., Zanzi, D., Ionna, F., Lombardi, M. L., Errico, S., ... Manzo, C. (2000). CD40 expressed on human melanoma cells mediates T cell co-stimulation and tumor cell growth. International Immunology, 12(6), 787-795.

CD40 expressed on human melanoma cells mediates T cell co-stimulation and tumor cell growth. / Pirozzi, Giuseppe; Lombari, Vincenza; Zanzi, Delia; Ionna, Franco; Lombardi, Maria Luisa; Errico, Simona; Ruggiero, Giuseppina; Manzo, Ciro.

In: International Immunology, Vol. 12, No. 6, 2000, p. 787-795.

Research output: Contribution to journalArticle

Pirozzi, G, Lombari, V, Zanzi, D, Ionna, F, Lombardi, ML, Errico, S, Ruggiero, G & Manzo, C 2000, 'CD40 expressed on human melanoma cells mediates T cell co-stimulation and tumor cell growth', International Immunology, vol. 12, no. 6, pp. 787-795.
Pirozzi G, Lombari V, Zanzi D, Ionna F, Lombardi ML, Errico S et al. CD40 expressed on human melanoma cells mediates T cell co-stimulation and tumor cell growth. International Immunology. 2000;12(6):787-795.
Pirozzi, Giuseppe ; Lombari, Vincenza ; Zanzi, Delia ; Ionna, Franco ; Lombardi, Maria Luisa ; Errico, Simona ; Ruggiero, Giuseppina ; Manzo, Ciro. / CD40 expressed on human melanoma cells mediates T cell co-stimulation and tumor cell growth. In: International Immunology. 2000 ; Vol. 12, No. 6. pp. 787-795.
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AU - Lombardi, Maria Luisa

AU - Errico, Simona

AU - Ruggiero, Giuseppina

AU - Manzo, Ciro

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