CD4(+)CD25(hi)CD127(-) Treg and CD4(+)CD45R0(+)CD49b(+)LAG3(+) Tr1 cells in bone marrow and peripheral blood samples from children with neuroblastoma

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Abstract

Metastatic spread in the bone marrow (BM) at diagnosis is the worst prognostic factor for neuroblastoma (NB) patients. Here, we analyzed the presence of two immunosuppressive cell subsets, CD4(+)CD25(hi)CD127(-) regulatory T (Treg) cells and CD4(+)CD45R0(+)CD49b(+)LAG3(+) type 1 regulatory (Tr1) cells, in BM and peripheral blood (PB) samples from NB patients and controls. Frequency of both regulatory cell subsets was lower in BM and PB samples from NB patients than in respective healthy controls. No correlation was found between the frequency of Treg and Tr1 cells and prognostic factors at diagnosis, such as age and stage. Only MYCN amplification correlated to a higher number of Treg in BM and of Tr1 in PB. These findings suggested an altered trafficking of regulatory T cells in NB, but delineated a limited role of these subsets in BM microenvironment and/or periphery in NB. These observations should be considered designing immunotherapeutic approaches for metastatic NB.

Original languageEnglish
Pages (from-to)e1249553
JournalOncoImmunology
Volume5
Issue number12
DOIs
Publication statusPublished - 2016

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Regulatory T-Lymphocytes
Neuroblastoma
Bone Marrow
Immunosuppressive Agents

Keywords

  • Journal Article

Cite this

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title = "CD4(+)CD25(hi)CD127(-) Treg and CD4(+)CD45R0(+)CD49b(+)LAG3(+) Tr1 cells in bone marrow and peripheral blood samples from children with neuroblastoma",
abstract = "Metastatic spread in the bone marrow (BM) at diagnosis is the worst prognostic factor for neuroblastoma (NB) patients. Here, we analyzed the presence of two immunosuppressive cell subsets, CD4(+)CD25(hi)CD127(-) regulatory T (Treg) cells and CD4(+)CD45R0(+)CD49b(+)LAG3(+) type 1 regulatory (Tr1) cells, in BM and peripheral blood (PB) samples from NB patients and controls. Frequency of both regulatory cell subsets was lower in BM and PB samples from NB patients than in respective healthy controls. No correlation was found between the frequency of Treg and Tr1 cells and prognostic factors at diagnosis, such as age and stage. Only MYCN amplification correlated to a higher number of Treg in BM and of Tr1 in PB. These findings suggested an altered trafficking of regulatory T cells in NB, but delineated a limited role of these subsets in BM microenvironment and/or periphery in NB. These observations should be considered designing immunotherapeutic approaches for metastatic NB.",
keywords = "Journal Article",
author = "Fabio Morandi and Sarah Pozzi and Sebastiano Barco and Giuliana Cangemi and Loredana Amoroso and Barbara Carlini and Vito Pistoia and Corrias, {Maria Valeria}",
year = "2016",
doi = "10.1080/2162402X.2016.1249553",
language = "English",
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pages = "e1249553",
journal = "OncoImmunology",
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TY - JOUR

T1 - CD4(+)CD25(hi)CD127(-) Treg and CD4(+)CD45R0(+)CD49b(+)LAG3(+) Tr1 cells in bone marrow and peripheral blood samples from children with neuroblastoma

AU - Morandi, Fabio

AU - Pozzi, Sarah

AU - Barco, Sebastiano

AU - Cangemi, Giuliana

AU - Amoroso, Loredana

AU - Carlini, Barbara

AU - Pistoia, Vito

AU - Corrias, Maria Valeria

PY - 2016

Y1 - 2016

N2 - Metastatic spread in the bone marrow (BM) at diagnosis is the worst prognostic factor for neuroblastoma (NB) patients. Here, we analyzed the presence of two immunosuppressive cell subsets, CD4(+)CD25(hi)CD127(-) regulatory T (Treg) cells and CD4(+)CD45R0(+)CD49b(+)LAG3(+) type 1 regulatory (Tr1) cells, in BM and peripheral blood (PB) samples from NB patients and controls. Frequency of both regulatory cell subsets was lower in BM and PB samples from NB patients than in respective healthy controls. No correlation was found between the frequency of Treg and Tr1 cells and prognostic factors at diagnosis, such as age and stage. Only MYCN amplification correlated to a higher number of Treg in BM and of Tr1 in PB. These findings suggested an altered trafficking of regulatory T cells in NB, but delineated a limited role of these subsets in BM microenvironment and/or periphery in NB. These observations should be considered designing immunotherapeutic approaches for metastatic NB.

AB - Metastatic spread in the bone marrow (BM) at diagnosis is the worst prognostic factor for neuroblastoma (NB) patients. Here, we analyzed the presence of two immunosuppressive cell subsets, CD4(+)CD25(hi)CD127(-) regulatory T (Treg) cells and CD4(+)CD45R0(+)CD49b(+)LAG3(+) type 1 regulatory (Tr1) cells, in BM and peripheral blood (PB) samples from NB patients and controls. Frequency of both regulatory cell subsets was lower in BM and PB samples from NB patients than in respective healthy controls. No correlation was found between the frequency of Treg and Tr1 cells and prognostic factors at diagnosis, such as age and stage. Only MYCN amplification correlated to a higher number of Treg in BM and of Tr1 in PB. These findings suggested an altered trafficking of regulatory T cells in NB, but delineated a limited role of these subsets in BM microenvironment and/or periphery in NB. These observations should be considered designing immunotherapeutic approaches for metastatic NB.

KW - Journal Article

U2 - 10.1080/2162402X.2016.1249553

DO - 10.1080/2162402X.2016.1249553

M3 - Article

VL - 5

SP - e1249553

JO - OncoImmunology

JF - OncoImmunology

SN - 2162-4011

IS - 12

ER -