Cd56 homodimerization and participation in anti-tumor immune effector cell functioning: A role for interleukin-15

Heleen H. Van Acker, Zoë P. Van Acker, Maarten Versteven, Peter Ponsaerts, Daniela Pende, Zwi N. Berneman, Sébastien Anguille, Viggo F. Van Tendeloo, Evelien L. Smits

Research output: Contribution to journalArticle

Abstract

A particularly interesting marker to identify anti-tumor immune cells is the neural cell adhesion molecule (NCAM), also known as cluster of differentiation (CD)56. Namely, hematopoietic expression of CD56 seems to be confined to powerful effector immune cells. Here, we sought to elucidate its role on various killer immune cells. First, we identified the high motility NCAM-120 molecule to be the main isoform expressed by immune cells. Next, through neutralization of surface CD56, we were able to (1) demonstrate the direct involvement of CD56 in tumor cell lysis exerted by CD56-expressing killer cells, such as natural killer cells, gamma delta (γδ) T cells, and interleukin (IL)-15-cultured dendritic cells (DCs), and (2) reveal a putative crosstalk mechanism between IL-15 DCs and CD8 T cells, suggesting CD56 as a co-stimulatory molecule in their cell-to-cell contact. Moreover, by means of a proximity ligation assay, we visualized the CD56 homophilic interaction among cancer cells and between immune cells and cancer cells. Finally, by blocking the mitogen-activated protein kinase (MAPK) pathway and the phosphoinositide 3-kinase (PI3K)–Akt pathway, we showed that IL-15 stimulation directly led to CD56 upregulation. In conclusion, these results underscore the previously neglected importance of CD56 expression on immune cells, benefiting current and future immune therapeutic options.

Original languageEnglish
Article number1029
JournalCancers
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 1 2019

Fingerprint

Interleukin-15
Neoplasms
Neural Cell Adhesion Molecules
Dendritic Cells
T-Lymphocytes
1-Phosphatidylinositol 4-Kinase
Somatostatin-Secreting Cells
Mitogen-Activated Protein Kinases
Natural Killer Cells
Ligation
Cultured Cells
Protein Isoforms
Up-Regulation

Keywords

  • CD56 homodimers
  • Common gamma-chain family
  • Interleukin-15 signaling
  • NCAM-120
  • Tumor cell eradication

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Van Acker, H. H., Van Acker, Z. P., Versteven, M., Ponsaerts, P., Pende, D., Berneman, Z. N., ... Smits, E. L. (2019). Cd56 homodimerization and participation in anti-tumor immune effector cell functioning: A role for interleukin-15. Cancers, 11(7), [1029]. https://doi.org/10.3390/cancers11071029

Cd56 homodimerization and participation in anti-tumor immune effector cell functioning : A role for interleukin-15. / Van Acker, Heleen H.; Van Acker, Zoë P.; Versteven, Maarten; Ponsaerts, Peter; Pende, Daniela; Berneman, Zwi N.; Anguille, Sébastien; Van Tendeloo, Viggo F.; Smits, Evelien L.

In: Cancers, Vol. 11, No. 7, 1029, 01.07.2019.

Research output: Contribution to journalArticle

Van Acker, HH, Van Acker, ZP, Versteven, M, Ponsaerts, P, Pende, D, Berneman, ZN, Anguille, S, Van Tendeloo, VF & Smits, EL 2019, 'Cd56 homodimerization and participation in anti-tumor immune effector cell functioning: A role for interleukin-15', Cancers, vol. 11, no. 7, 1029. https://doi.org/10.3390/cancers11071029
Van Acker, Heleen H. ; Van Acker, Zoë P. ; Versteven, Maarten ; Ponsaerts, Peter ; Pende, Daniela ; Berneman, Zwi N. ; Anguille, Sébastien ; Van Tendeloo, Viggo F. ; Smits, Evelien L. / Cd56 homodimerization and participation in anti-tumor immune effector cell functioning : A role for interleukin-15. In: Cancers. 2019 ; Vol. 11, No. 7.
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