CD56 is a pathogen recognition receptor on human natural killer cells

Sabrina Ziegler, Esther Weiss, Anna Lena Schmitt, Jan Schlegel, Anne Burgert, Ulrich Terpitz, Markus Sauer, Lorenzo Moretta, Simona Sivori, Ines Leonhardt, Oliver Kurzai, Hermann Einsele, Juergen Loeffler

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aspergillus (A.) fumigatus is an opportunistic fungal mold inducing invasive aspergillosis (IA) in immunocompromised patients. Although antifungal activity of human natural killer (NK) cells was shown in previous studies, the underlying cellular mechanisms and pathogen recognition receptors (PRRs) are still unknown. Using flow cytometry we were able to show that the fluorescence positivity of the surface receptor CD56 significantly decreased upon fungal contact. To visualize the interaction site of NK cells and A. fumigatus we used SEM, CLSM and dSTORM techniques, which clearly demonstrated that NK cells directly interact with A. fumigatus via CD56 and that CD56 is re-organized and accumulated at this interaction site time-dependently. The inhibition of the cytoskeleton showed that the receptor re-organization was an active process dependent on actin re-arrangements. Furthermore, we could show that CD56 plays a role in the fungus mediated NK cell activation, since blocking of CD56 surface receptor reduced fungal mediated NK cell activation and reduced cytokine secretion. These results confirmed the direct interaction of NK cells and A. fumigatus, leading to the conclusion that CD56 is a pathogen recognition receptor. These findings give new insights into the functional role of CD56 in the pathogen recognition during the innate immune response.

Original languageEnglish
Article number6138
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Natural Killer Cells
Fungi
Aspergillus fumigatus
Aspergillosis
Immunocompromised Host
Cytoskeleton
Innate Immunity
Human Activities
Actins
Flow Cytometry
Fluorescence
Cytokines

ASJC Scopus subject areas

  • General

Cite this

Ziegler, S., Weiss, E., Schmitt, A. L., Schlegel, J., Burgert, A., Terpitz, U., ... Loeffler, J. (2017). CD56 is a pathogen recognition receptor on human natural killer cells. Scientific Reports, 7(1), [6138]. https://doi.org/10.1038/s41598-017-06238-4

CD56 is a pathogen recognition receptor on human natural killer cells. / Ziegler, Sabrina; Weiss, Esther; Schmitt, Anna Lena; Schlegel, Jan; Burgert, Anne; Terpitz, Ulrich; Sauer, Markus; Moretta, Lorenzo; Sivori, Simona; Leonhardt, Ines; Kurzai, Oliver; Einsele, Hermann; Loeffler, Juergen.

In: Scientific Reports, Vol. 7, No. 1, 6138, 01.12.2017.

Research output: Contribution to journalArticle

Ziegler, S, Weiss, E, Schmitt, AL, Schlegel, J, Burgert, A, Terpitz, U, Sauer, M, Moretta, L, Sivori, S, Leonhardt, I, Kurzai, O, Einsele, H & Loeffler, J 2017, 'CD56 is a pathogen recognition receptor on human natural killer cells', Scientific Reports, vol. 7, no. 1, 6138. https://doi.org/10.1038/s41598-017-06238-4
Ziegler S, Weiss E, Schmitt AL, Schlegel J, Burgert A, Terpitz U et al. CD56 is a pathogen recognition receptor on human natural killer cells. Scientific Reports. 2017 Dec 1;7(1). 6138. https://doi.org/10.1038/s41598-017-06238-4
Ziegler, Sabrina ; Weiss, Esther ; Schmitt, Anna Lena ; Schlegel, Jan ; Burgert, Anne ; Terpitz, Ulrich ; Sauer, Markus ; Moretta, Lorenzo ; Sivori, Simona ; Leonhardt, Ines ; Kurzai, Oliver ; Einsele, Hermann ; Loeffler, Juergen. / CD56 is a pathogen recognition receptor on human natural killer cells. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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