Abstract
As limited information is available regarding the distribution and trafficking of NK cells among solid organs, we have analyzed a wide array of tissues derived from different human compartments. NK cells were widely distributed in most solid tissues, although their amount varied significantly depending on the tissue/organ analyzed. Interestingly, the distribution appeared to be subset specific, as some tissues were preferentially populated by CD56brightperforinlowNK cells, with others by the CD56dimperforinhigh cytotoxic counterpart. Nevertheless, most tissues were highly enriched in CD56brightperforin lowcells, and the distribution of NK subsets appeared in accordance with tissue gene expression of chemotactic factors, for which receptors are differently represented in the two subsets. Remarkably, chemokine expression pattern of tissues was modified after neoplastic transformation. As a result, although the total amount of NK cells infiltrating the tissues did not significantly change upon malignant transformation, the relative proportion of NK subsets infiltrating the tissues was different, with a trend toward a tumor-infiltrating NK population enriched in noncytotoxic cells. Besides solid tissues, CD56brightperforinlowNK cells were also detected in seroma fluids, which represents an accrual of human afferent lymph, indicating that they may leave peripheral solid tissues and recirculate to secondary lymphoid organs via lymphatic vessels. Our results provide a comprehensive mapping of NK cells in human tissues, demonstrating that discrete NK subsets populate and recirculate through most human tissues and that organ-specific chemokine expression patterns might affect their distribution. In this context, chemokine switch upon neoplastic transformation might represent a novel mechanism of tumor immune escape.
| Original language | English |
|---|---|
| Pages (from-to) | 3805-3815 |
| Number of pages | 11 |
| Journal | Journal of Immunology |
| Volume | 192 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Apr 15 2014 |
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ASJC Scopus subject areas
- Immunology
Cite this
CD56brightperforinlow noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph. / Carrega, Paolo; Bonaccorsi, Irene; Di Carlo, Emma; Morandi, Barbara; Paul, Petra; Rizzello, Valeria; Cipollone, Giuseppe; Navarra, Giuseppe; Mingari, Maria Cristina; Moretta, Lorenzo; Ferlazzo, Guido.
In: Journal of Immunology, Vol. 192, No. 8, 15.04.2014, p. 3805-3815.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CD56brightperforinlow noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph
AU - Carrega, Paolo
AU - Bonaccorsi, Irene
AU - Di Carlo, Emma
AU - Morandi, Barbara
AU - Paul, Petra
AU - Rizzello, Valeria
AU - Cipollone, Giuseppe
AU - Navarra, Giuseppe
AU - Mingari, Maria Cristina
AU - Moretta, Lorenzo
AU - Ferlazzo, Guido
PY - 2014/4/15
Y1 - 2014/4/15
N2 - As limited information is available regarding the distribution and trafficking of NK cells among solid organs, we have analyzed a wide array of tissues derived from different human compartments. NK cells were widely distributed in most solid tissues, although their amount varied significantly depending on the tissue/organ analyzed. Interestingly, the distribution appeared to be subset specific, as some tissues were preferentially populated by CD56brightperforinlowNK cells, with others by the CD56dimperforinhigh cytotoxic counterpart. Nevertheless, most tissues were highly enriched in CD56brightperforin lowcells, and the distribution of NK subsets appeared in accordance with tissue gene expression of chemotactic factors, for which receptors are differently represented in the two subsets. Remarkably, chemokine expression pattern of tissues was modified after neoplastic transformation. As a result, although the total amount of NK cells infiltrating the tissues did not significantly change upon malignant transformation, the relative proportion of NK subsets infiltrating the tissues was different, with a trend toward a tumor-infiltrating NK population enriched in noncytotoxic cells. Besides solid tissues, CD56brightperforinlowNK cells were also detected in seroma fluids, which represents an accrual of human afferent lymph, indicating that they may leave peripheral solid tissues and recirculate to secondary lymphoid organs via lymphatic vessels. Our results provide a comprehensive mapping of NK cells in human tissues, demonstrating that discrete NK subsets populate and recirculate through most human tissues and that organ-specific chemokine expression patterns might affect their distribution. In this context, chemokine switch upon neoplastic transformation might represent a novel mechanism of tumor immune escape.
AB - As limited information is available regarding the distribution and trafficking of NK cells among solid organs, we have analyzed a wide array of tissues derived from different human compartments. NK cells were widely distributed in most solid tissues, although their amount varied significantly depending on the tissue/organ analyzed. Interestingly, the distribution appeared to be subset specific, as some tissues were preferentially populated by CD56brightperforinlowNK cells, with others by the CD56dimperforinhigh cytotoxic counterpart. Nevertheless, most tissues were highly enriched in CD56brightperforin lowcells, and the distribution of NK subsets appeared in accordance with tissue gene expression of chemotactic factors, for which receptors are differently represented in the two subsets. Remarkably, chemokine expression pattern of tissues was modified after neoplastic transformation. As a result, although the total amount of NK cells infiltrating the tissues did not significantly change upon malignant transformation, the relative proportion of NK subsets infiltrating the tissues was different, with a trend toward a tumor-infiltrating NK population enriched in noncytotoxic cells. Besides solid tissues, CD56brightperforinlowNK cells were also detected in seroma fluids, which represents an accrual of human afferent lymph, indicating that they may leave peripheral solid tissues and recirculate to secondary lymphoid organs via lymphatic vessels. Our results provide a comprehensive mapping of NK cells in human tissues, demonstrating that discrete NK subsets populate and recirculate through most human tissues and that organ-specific chemokine expression patterns might affect their distribution. In this context, chemokine switch upon neoplastic transformation might represent a novel mechanism of tumor immune escape.
UR - http://www.scopus.com/inward/record.url?scp=84898619037&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84898619037&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.1301889
DO - 10.4049/jimmunol.1301889
M3 - Article
C2 - 24646734
AN - SCOPUS:84898619037
VL - 192
SP - 3805
EP - 3815
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 8
ER -