CD56brightperforinlow noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph

Paolo Carrega, Irene Bonaccorsi, Emma Di Carlo, Barbara Morandi, Petra Paul, Valeria Rizzello, Giuseppe Cipollone, Giuseppe Navarra, Maria Cristina Mingari, Lorenzo Moretta, Guido Ferlazzo

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Abstract

As limited information is available regarding the distribution and trafficking of NK cells among solid organs, we have analyzed a wide array of tissues derived from different human compartments. NK cells were widely distributed in most solid tissues, although their amount varied significantly depending on the tissue/organ analyzed. Interestingly, the distribution appeared to be subset specific, as some tissues were preferentially populated by CD56brightperforinlowNK cells, with others by the CD56dimperforinhigh cytotoxic counterpart. Nevertheless, most tissues were highly enriched in CD56brightperforin lowcells, and the distribution of NK subsets appeared in accordance with tissue gene expression of chemotactic factors, for which receptors are differently represented in the two subsets. Remarkably, chemokine expression pattern of tissues was modified after neoplastic transformation. As a result, although the total amount of NK cells infiltrating the tissues did not significantly change upon malignant transformation, the relative proportion of NK subsets infiltrating the tissues was different, with a trend toward a tumor-infiltrating NK population enriched in noncytotoxic cells. Besides solid tissues, CD56brightperforinlowNK cells were also detected in seroma fluids, which represents an accrual of human afferent lymph, indicating that they may leave peripheral solid tissues and recirculate to secondary lymphoid organs via lymphatic vessels. Our results provide a comprehensive mapping of NK cells in human tissues, demonstrating that discrete NK subsets populate and recirculate through most human tissues and that organ-specific chemokine expression patterns might affect their distribution. In this context, chemokine switch upon neoplastic transformation might represent a novel mechanism of tumor immune escape.

Original languageEnglish
Pages (from-to)3805-3815
Number of pages11
JournalJournal of Immunology
Volume192
Issue number8
DOIs
Publication statusPublished - Apr 15 2014

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Lymph
Natural Killer Cells
Chemokines
Tumor Escape
Seroma
Lymphatic Vessels
Chemotactic Factors

ASJC Scopus subject areas

  • Immunology

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CD56brightperforinlow noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph. / Carrega, Paolo; Bonaccorsi, Irene; Di Carlo, Emma; Morandi, Barbara; Paul, Petra; Rizzello, Valeria; Cipollone, Giuseppe; Navarra, Giuseppe; Mingari, Maria Cristina; Moretta, Lorenzo; Ferlazzo, Guido.

In: Journal of Immunology, Vol. 192, No. 8, 15.04.2014, p. 3805-3815.

Research output: Contribution to journalArticle

Carrega, Paolo ; Bonaccorsi, Irene ; Di Carlo, Emma ; Morandi, Barbara ; Paul, Petra ; Rizzello, Valeria ; Cipollone, Giuseppe ; Navarra, Giuseppe ; Mingari, Maria Cristina ; Moretta, Lorenzo ; Ferlazzo, Guido. / CD56brightperforinlow noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph. In: Journal of Immunology. 2014 ; Vol. 192, No. 8. pp. 3805-3815.
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AU - Carrega, Paolo

AU - Bonaccorsi, Irene

AU - Di Carlo, Emma

AU - Morandi, Barbara

AU - Paul, Petra

AU - Rizzello, Valeria

AU - Cipollone, Giuseppe

AU - Navarra, Giuseppe

AU - Mingari, Maria Cristina

AU - Moretta, Lorenzo

AU - Ferlazzo, Guido

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