CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses

Teresa Carbone, Francesca Nasorri, Davide Pennino, Kilian Eyerich, Stefanie Foerster, Loredana Cifaldi, Claudia Traidl-Hoffman, Heidrun Behrendt, Andrea Cavani

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Abstract

Allergic contact dermatitis is a common disease caused by an exaggerated T cell-mediated immune response to skin-applied haptens. We show in this study that NK cells affect skin immune responses to haptens by releasing type 1 cytokines and inducing keratinocytes apoptosis. Immunohistochemical stainings demonstrated thatNKlymphocytes constitute ∼10%of the inflammatory infiltrate mostly distributed in the superficial dermis and in the epidermis at the site of intense spongiotic changes. More than 90% of NK cells isolated from allergic contact dermatitis skin showed a CD3-CD56highCD16- phenotype by FACS analysis. In addition, they uniformly expressed NKG2A, intermediate to high levels of perforin, and the activating receptors, NKG2D, NKp44, and NKp46, but lacked NKp30 and killer Ig-related receptors. Skin NK lymphocytes displayed a CXCR3+CCR6+CCR5+ chemokine receptor asset for homing into inflamed skin, but not CD62L and CCR7 for lymph node homing. When NK cells from nickel-allergic donors were exposed in vitro to the metal, they failed to proliferate, to upregulate CD69, and to release IFN-γ, thus indicating that NK lymphocytes do not exhibit memory-like properties to haptens. However, IL-2 released by hapten-driven T lymphocytes rapidly induced the release of IFN-γ byNKcells and promoted the NK-mediated apoptosis of autologous keratinocytes in a hapten-independent manner. Our findings underline the importance of the interaction between innate and adaptive immune mechanisms for amplification of skin allergic responses to haptens and full expression of allergic contact dermatitis.

Original languageEnglish
Pages (from-to)1102-1110
Number of pages9
JournalJournal of Immunology
Volume184
Issue number2
DOIs
Publication statusPublished - Jan 15 2010

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ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Carbone, T., Nasorri, F., Pennino, D., Eyerich, K., Foerster, S., Cifaldi, L., Traidl-Hoffman, C., Behrendt, H., & Cavani, A. (2010). CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses. Journal of Immunology, 184(2), 1102-1110. https://doi.org/10.4049/jimmunol.0902518