CD62, thromboxane B2, and beta-thromboglobulin: A comparison between different markers of platelet activation after contact with biomaterials

E. Cenni, D. Granchi, E. Verri, D. Cavedagna, S. Gamberini, G. Falsone, A. Pizzoferrato

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The authors examined the modifications of some markers of platelet activation after contact with biomaterials. Glycoprotein GMP-140 (CD62) was evaluated by flow cytometry; β-thromboglobulin (β-TG) and thromboxane B2 (TXB2) were determined by radioimmunoassay. Polyethylene terephthalate (PET) induced a remarkable platelet adhesion and a significant increase in β-TG and TXB2, with no increase in CD62 on the nonadherent platelets. Pyrolytic carbon-coated PET (PC) did not induce platelet adhesion after 15 min of contact, but a significant increase in CD62 was detected. After 30 min a significant increase in platelet adhesion as well as the release of β-TG and TXB2 were noted. The increase was lower than that observed for uncoated PET, and after 30 min of contact with PC the increase no longer was observed.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalJournal of Biomedical Materials Research
Volume36
Issue number3
DOIs
Publication statusPublished - Sep 5 1997

Fingerprint

beta-Thromboglobulin
Thromboxane B2
Platelet Activation
Biocompatible Materials
Platelets
Biomaterials
Polyethylene Terephthalates
Blood Platelets
Chemical activation
Polyethylene terephthalates
Adhesion
P-Selectin
Glycoproteins
Carbon
Flow cytometry
Radioimmunoassay
Flow Cytometry
pyrolytic carbon

Keywords

  • Biomaterial
  • GMP-140
  • Platelet
  • Release reaction

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

CD62, thromboxane B2, and beta-thromboglobulin : A comparison between different markers of platelet activation after contact with biomaterials. / Cenni, E.; Granchi, D.; Verri, E.; Cavedagna, D.; Gamberini, S.; Falsone, G.; Pizzoferrato, A.

In: Journal of Biomedical Materials Research, Vol. 36, No. 3, 05.09.1997, p. 289-294.

Research output: Contribution to journalArticle

Cenni, E. ; Granchi, D. ; Verri, E. ; Cavedagna, D. ; Gamberini, S. ; Falsone, G. ; Pizzoferrato, A. / CD62, thromboxane B2, and beta-thromboglobulin : A comparison between different markers of platelet activation after contact with biomaterials. In: Journal of Biomedical Materials Research. 1997 ; Vol. 36, No. 3. pp. 289-294.
@article{a41ae33ca364469cb44564ae323e2226,
title = "CD62, thromboxane B2, and beta-thromboglobulin: A comparison between different markers of platelet activation after contact with biomaterials",
abstract = "The authors examined the modifications of some markers of platelet activation after contact with biomaterials. Glycoprotein GMP-140 (CD62) was evaluated by flow cytometry; β-thromboglobulin (β-TG) and thromboxane B2 (TXB2) were determined by radioimmunoassay. Polyethylene terephthalate (PET) induced a remarkable platelet adhesion and a significant increase in β-TG and TXB2, with no increase in CD62 on the nonadherent platelets. Pyrolytic carbon-coated PET (PC) did not induce platelet adhesion after 15 min of contact, but a significant increase in CD62 was detected. After 30 min a significant increase in platelet adhesion as well as the release of β-TG and TXB2 were noted. The increase was lower than that observed for uncoated PET, and after 30 min of contact with PC the increase no longer was observed.",
keywords = "Biomaterial, GMP-140, Platelet, Release reaction",
author = "E. Cenni and D. Granchi and E. Verri and D. Cavedagna and S. Gamberini and G. Falsone and A. Pizzoferrato",
year = "1997",
month = "9",
day = "5",
doi = "10.1002/(SICI)1097-4636(19970905)36:3<289::AID-JBM3>3.0.CO;2-A",
language = "English",
volume = "36",
pages = "289--294",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - CD62, thromboxane B2, and beta-thromboglobulin

T2 - A comparison between different markers of platelet activation after contact with biomaterials

AU - Cenni, E.

AU - Granchi, D.

AU - Verri, E.

AU - Cavedagna, D.

AU - Gamberini, S.

AU - Falsone, G.

AU - Pizzoferrato, A.

PY - 1997/9/5

Y1 - 1997/9/5

N2 - The authors examined the modifications of some markers of platelet activation after contact with biomaterials. Glycoprotein GMP-140 (CD62) was evaluated by flow cytometry; β-thromboglobulin (β-TG) and thromboxane B2 (TXB2) were determined by radioimmunoassay. Polyethylene terephthalate (PET) induced a remarkable platelet adhesion and a significant increase in β-TG and TXB2, with no increase in CD62 on the nonadherent platelets. Pyrolytic carbon-coated PET (PC) did not induce platelet adhesion after 15 min of contact, but a significant increase in CD62 was detected. After 30 min a significant increase in platelet adhesion as well as the release of β-TG and TXB2 were noted. The increase was lower than that observed for uncoated PET, and after 30 min of contact with PC the increase no longer was observed.

AB - The authors examined the modifications of some markers of platelet activation after contact with biomaterials. Glycoprotein GMP-140 (CD62) was evaluated by flow cytometry; β-thromboglobulin (β-TG) and thromboxane B2 (TXB2) were determined by radioimmunoassay. Polyethylene terephthalate (PET) induced a remarkable platelet adhesion and a significant increase in β-TG and TXB2, with no increase in CD62 on the nonadherent platelets. Pyrolytic carbon-coated PET (PC) did not induce platelet adhesion after 15 min of contact, but a significant increase in CD62 was detected. After 30 min a significant increase in platelet adhesion as well as the release of β-TG and TXB2 were noted. The increase was lower than that observed for uncoated PET, and after 30 min of contact with PC the increase no longer was observed.

KW - Biomaterial

KW - GMP-140

KW - Platelet

KW - Release reaction

UR - http://www.scopus.com/inward/record.url?scp=0031554924&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031554924&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4636(19970905)36:3<289::AID-JBM3>3.0.CO;2-A

DO - 10.1002/(SICI)1097-4636(19970905)36:3<289::AID-JBM3>3.0.CO;2-A

M3 - Article

C2 - 9260099

AN - SCOPUS:0031554924

VL - 36

SP - 289

EP - 294

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 3

ER -