Abstract
Objective. Since the early activation antigen CD69 has been implicated in the pathogenesis of some inflammatory diseases, we evaluated the expression of the molecule on peripheral blood (PB) and synovial fluid (SF) neutrophils obtained from RA patients and its possible correlation with PB and SF cytokine concentration. Methods. CD69 membrane expression (and CD11b as control marker) was assessed by indirect immunofluorescence and flow cytometry analysis on purified PB and SF neutrophils. Cytokine levels (GM-CSF, IFN-γ, TNF-α) in plasma and SF supernatants were measured by ELISA. Results. CD69 was absent on control neutrophils, while it was expressed on PB neutrophils from RA patients although no detectable GM-CSF, IFN-γ or TNF-α was observed in their plasma. CD69 expression was still more evident on SF neutrophils from RA patients; 59% had detectable levels of INF-γ in their SF while GM-CSF and TNF-α were detectable in SF from 95% and 33% of RA patients, respectively. However, no correlation was observed between cytokine concentrations and CD69 expression on SF neutrophils. SF but not PB neutrophils from RA patients expressed increased amounts of CD11b when compared to control PB neutrophils without any correlation with CD69 membrane expression. Conclusion. The activation antigen CD69 is significantly expressed on PB and SF neutrophils from RA patients. However, the mechanism(s) of induction and its possible role in the pathogenesis of RA remain to be defined.
| Original language | English |
|---|---|
| Pages (from-to) | 331-334 |
| Number of pages | 4 |
| Journal | Clinical and Experimental Rheumatology |
| Volume | 22 |
| Issue number | 3 |
| Publication status | Published - May 2004 |
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Keywords
- CD69
- Neutrophils
- Rheumatoid arthritis
ASJC Scopus subject areas
- Immunology
- Rheumatology
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CD69 expression on neutrophils from patients with rheumatoid arthritis. / Atzeni, Fabiola; Del Papa, Nicoletta; Sarzi-Puttini, Piercarlo; Bertolazzi, Francesca; Minonzio, Francesca; Capsoni, Franco.
In: Clinical and Experimental Rheumatology, Vol. 22, No. 3, 05.2004, p. 331-334.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CD69 expression on neutrophils from patients with rheumatoid arthritis
AU - Atzeni, Fabiola
AU - Del Papa, Nicoletta
AU - Sarzi-Puttini, Piercarlo
AU - Bertolazzi, Francesca
AU - Minonzio, Francesca
AU - Capsoni, Franco
PY - 2004/5
Y1 - 2004/5
N2 - Objective. Since the early activation antigen CD69 has been implicated in the pathogenesis of some inflammatory diseases, we evaluated the expression of the molecule on peripheral blood (PB) and synovial fluid (SF) neutrophils obtained from RA patients and its possible correlation with PB and SF cytokine concentration. Methods. CD69 membrane expression (and CD11b as control marker) was assessed by indirect immunofluorescence and flow cytometry analysis on purified PB and SF neutrophils. Cytokine levels (GM-CSF, IFN-γ, TNF-α) in plasma and SF supernatants were measured by ELISA. Results. CD69 was absent on control neutrophils, while it was expressed on PB neutrophils from RA patients although no detectable GM-CSF, IFN-γ or TNF-α was observed in their plasma. CD69 expression was still more evident on SF neutrophils from RA patients; 59% had detectable levels of INF-γ in their SF while GM-CSF and TNF-α were detectable in SF from 95% and 33% of RA patients, respectively. However, no correlation was observed between cytokine concentrations and CD69 expression on SF neutrophils. SF but not PB neutrophils from RA patients expressed increased amounts of CD11b when compared to control PB neutrophils without any correlation with CD69 membrane expression. Conclusion. The activation antigen CD69 is significantly expressed on PB and SF neutrophils from RA patients. However, the mechanism(s) of induction and its possible role in the pathogenesis of RA remain to be defined.
AB - Objective. Since the early activation antigen CD69 has been implicated in the pathogenesis of some inflammatory diseases, we evaluated the expression of the molecule on peripheral blood (PB) and synovial fluid (SF) neutrophils obtained from RA patients and its possible correlation with PB and SF cytokine concentration. Methods. CD69 membrane expression (and CD11b as control marker) was assessed by indirect immunofluorescence and flow cytometry analysis on purified PB and SF neutrophils. Cytokine levels (GM-CSF, IFN-γ, TNF-α) in plasma and SF supernatants were measured by ELISA. Results. CD69 was absent on control neutrophils, while it was expressed on PB neutrophils from RA patients although no detectable GM-CSF, IFN-γ or TNF-α was observed in their plasma. CD69 expression was still more evident on SF neutrophils from RA patients; 59% had detectable levels of INF-γ in their SF while GM-CSF and TNF-α were detectable in SF from 95% and 33% of RA patients, respectively. However, no correlation was observed between cytokine concentrations and CD69 expression on SF neutrophils. SF but not PB neutrophils from RA patients expressed increased amounts of CD11b when compared to control PB neutrophils without any correlation with CD69 membrane expression. Conclusion. The activation antigen CD69 is significantly expressed on PB and SF neutrophils from RA patients. However, the mechanism(s) of induction and its possible role in the pathogenesis of RA remain to be defined.
KW - CD69
KW - Neutrophils
KW - Rheumatoid arthritis
UR - http://www.scopus.com/inward/record.url?scp=2342474572&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2342474572&partnerID=8YFLogxK
M3 - Article
C2 - 15144128
AN - SCOPUS:2342474572
VL - 22
SP - 331
EP - 334
JO - Clinical and Experimental Rheumatology
JF - Clinical and Experimental Rheumatology
SN - 0392-856X
IS - 3
ER -