TY - JOUR
T1 - CD8+ NK cells are predominant in chimpanzees, characterized by high NCR expression and cytokine production, and preserved in chronic HIV-1 infection
AU - Rutjens, Erik
AU - Mazza, Stefania
AU - Biassoni, Roberto
AU - Koopman, Gerrit
AU - Ugolotti, Elisabetta
AU - Fogli, Manuela
AU - Dubbes, Rob
AU - Costa, Paola
AU - Mingari, Maria C.
AU - Greenwood, Edward J D
AU - Moretta, Lorenzo
AU - Demaria, Andrea
AU - Heeney, Jonathan L.
PY - 2010/5
Y1 - 2010/5
N2 - HIV-1 infection in humans results in an early and progressive NK cell dysfunction and an accumulation of an "anergic" CD56- CD16+ NK subset, which is characterised by low natural cytotoxicity receptor expression and low cytokine producing capacity. In contrast to humans, chimpanzee NK cells do not display a distinguishable CD56bright and CD56dim subset but, as shown here, could be subdivided into functionally different CD8+ and CD8- subsets. The CD8 + NK cells expressed significantly higher levels of triggering receptors including NKp46 and, upon in vitro activation, produced more IFN-γ, TNF-α and CD107 than their CD8- counterparts. In addition, chimpanzee CD8- NK cells had relatively high levels of HLA-DR expression, suggestive of an activated state. Killing inhibitory receptors were expressed only at low levels; however, upon in vitro stimulation, they were up-regulated in CD8+ but not in CD8- NK cells and were functionally capable of inhibiting NKp30-triggered killing. In contrast to HIV-1-infected humans, infected chimpanzees maintained their dominant CD8+ NK cell population, with high expression of natural cytotoxicity receptors.
AB - HIV-1 infection in humans results in an early and progressive NK cell dysfunction and an accumulation of an "anergic" CD56- CD16+ NK subset, which is characterised by low natural cytotoxicity receptor expression and low cytokine producing capacity. In contrast to humans, chimpanzee NK cells do not display a distinguishable CD56bright and CD56dim subset but, as shown here, could be subdivided into functionally different CD8+ and CD8- subsets. The CD8 + NK cells expressed significantly higher levels of triggering receptors including NKp46 and, upon in vitro activation, produced more IFN-γ, TNF-α and CD107 than their CD8- counterparts. In addition, chimpanzee CD8- NK cells had relatively high levels of HLA-DR expression, suggestive of an activated state. Killing inhibitory receptors were expressed only at low levels; however, upon in vitro stimulation, they were up-regulated in CD8+ but not in CD8- NK cells and were functionally capable of inhibiting NKp30-triggered killing. In contrast to HIV-1-infected humans, infected chimpanzees maintained their dominant CD8+ NK cell population, with high expression of natural cytotoxicity receptors.
KW - Chimpanzee
KW - HIV
KW - Natural cytotoxicity receptors (NCR)
KW - NK cell
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U2 - 10.1002/eji.200940062
DO - 10.1002/eji.200940062
M3 - Article
C2 - 20306468
AN - SCOPUS:77951742350
VL - 40
SP - 1440
EP - 1450
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 5
ER -