CD8+ T lymphocytes provide helper activity for IgE synthesis in human immunodeficiency virus-infected patients with hyper-IgE

Roberto Paganelli, Enrico Scala, Ignacio J. Ansotegui, Clara M. Ausiello, Eva Halapi, Emanuele Fanales-Belasio, Giampiero D'Offizi, Ivano Mezzaroma, Franco Pandolfi, Massimo Fiorilli, Antonio Cassone, Fernando Aiuti

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Increased levels of serum IgE and eosinophilia have been described in human immunodeficiency virus (HIV) infection, almost exclusively in patients with CD4+ cell count + T helper type 2 (Th-2) lymphocytes, producing interleukin 4 (IL-4) and expressing a ligand for the B cell-specific CD40 molecule (CD40 ligand [L]). A shift to a Th-2-like pattern of cytokine secretion has been postulated to be associated with progression toward acquired immunodeficiency syndrome (AIDS). We studied three AIDS patients with very high levels of IgE and almost complete depletion of CD4+ lymphocytes, suggesting that IgE synthesis could not be driven by CD4+ cells. IgE in vitro synthesis by cells from such patients was, however, inhibited by anti-IL-4. We show that both CD8+ T cell lines and majority of CD8+ T cell clones derived from these patients produce IL-4, IL-5, and IL-6 in half of the cases together with interferon γ (IFN- γ). 44% of CD8- T cell clones expressed a CD40L, and the supernatants of the clones were capable of inducing IgE synthesis by B cells costimulated with anti-CD40. CD8+ T cells in these patients therefore functionally mimic Th-2 type cells and may account for hyper-IgE and eosinophilia in the absence of CD4+ cells. The presence of such CD8+ cells may also provide a source of IL-4 directing the development of predominant Th-2 responses in HIV infection.

Original languageEnglish
Pages (from-to)423-428
Number of pages6
JournalJournal of Experimental Medicine
Volume181
Issue number1
DOIs
Publication statusPublished - Jan 1 1995

Fingerprint

Helper-Inducer T-Lymphocytes
Immunoglobulin E
HIV
Interleukin-4
T-Lymphocytes
CD40 Ligand
Clone Cells
Eosinophilia
Virus Diseases
Acquired Immunodeficiency Syndrome
B-Lymphocytes
Lymphocyte Depletion
Th2 Cells
Interleukin-5
CD4 Lymphocyte Count
Interferons
Interleukin-6
Cytokines
Ligands
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

CD8+ T lymphocytes provide helper activity for IgE synthesis in human immunodeficiency virus-infected patients with hyper-IgE. / Paganelli, Roberto; Scala, Enrico; Ansotegui, Ignacio J.; Ausiello, Clara M.; Halapi, Eva; Fanales-Belasio, Emanuele; D'Offizi, Giampiero; Mezzaroma, Ivano; Pandolfi, Franco; Fiorilli, Massimo; Cassone, Antonio; Aiuti, Fernando.

In: Journal of Experimental Medicine, Vol. 181, No. 1, 01.01.1995, p. 423-428.

Research output: Contribution to journalArticle

Paganelli, R, Scala, E, Ansotegui, IJ, Ausiello, CM, Halapi, E, Fanales-Belasio, E, D'Offizi, G, Mezzaroma, I, Pandolfi, F, Fiorilli, M, Cassone, A & Aiuti, F 1995, 'CD8+ T lymphocytes provide helper activity for IgE synthesis in human immunodeficiency virus-infected patients with hyper-IgE', Journal of Experimental Medicine, vol. 181, no. 1, pp. 423-428. https://doi.org/10.1084/jem.181.1.423
Paganelli, Roberto ; Scala, Enrico ; Ansotegui, Ignacio J. ; Ausiello, Clara M. ; Halapi, Eva ; Fanales-Belasio, Emanuele ; D'Offizi, Giampiero ; Mezzaroma, Ivano ; Pandolfi, Franco ; Fiorilli, Massimo ; Cassone, Antonio ; Aiuti, Fernando. / CD8+ T lymphocytes provide helper activity for IgE synthesis in human immunodeficiency virus-infected patients with hyper-IgE. In: Journal of Experimental Medicine. 1995 ; Vol. 181, No. 1. pp. 423-428.
@article{5a8c580cf4334f848666159fd66ab533,
title = "CD8+ T lymphocytes provide helper activity for IgE synthesis in human immunodeficiency virus-infected patients with hyper-IgE",
abstract = "Increased levels of serum IgE and eosinophilia have been described in human immunodeficiency virus (HIV) infection, almost exclusively in patients with CD4+ cell count + T helper type 2 (Th-2) lymphocytes, producing interleukin 4 (IL-4) and expressing a ligand for the B cell-specific CD40 molecule (CD40 ligand [L]). A shift to a Th-2-like pattern of cytokine secretion has been postulated to be associated with progression toward acquired immunodeficiency syndrome (AIDS). We studied three AIDS patients with very high levels of IgE and almost complete depletion of CD4+ lymphocytes, suggesting that IgE synthesis could not be driven by CD4+ cells. IgE in vitro synthesis by cells from such patients was, however, inhibited by anti-IL-4. We show that both CD8+ T cell lines and majority of CD8+ T cell clones derived from these patients produce IL-4, IL-5, and IL-6 in half of the cases together with interferon γ (IFN- γ). 44{\%} of CD8- T cell clones expressed a CD40L, and the supernatants of the clones were capable of inducing IgE synthesis by B cells costimulated with anti-CD40. CD8+ T cells in these patients therefore functionally mimic Th-2 type cells and may account for hyper-IgE and eosinophilia in the absence of CD4+ cells. The presence of such CD8+ cells may also provide a source of IL-4 directing the development of predominant Th-2 responses in HIV infection.",
author = "Roberto Paganelli and Enrico Scala and Ansotegui, {Ignacio J.} and Ausiello, {Clara M.} and Eva Halapi and Emanuele Fanales-Belasio and Giampiero D'Offizi and Ivano Mezzaroma and Franco Pandolfi and Massimo Fiorilli and Antonio Cassone and Fernando Aiuti",
year = "1995",
month = "1",
day = "1",
doi = "10.1084/jem.181.1.423",
language = "English",
volume = "181",
pages = "423--428",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - CD8+ T lymphocytes provide helper activity for IgE synthesis in human immunodeficiency virus-infected patients with hyper-IgE

AU - Paganelli, Roberto

AU - Scala, Enrico

AU - Ansotegui, Ignacio J.

AU - Ausiello, Clara M.

AU - Halapi, Eva

AU - Fanales-Belasio, Emanuele

AU - D'Offizi, Giampiero

AU - Mezzaroma, Ivano

AU - Pandolfi, Franco

AU - Fiorilli, Massimo

AU - Cassone, Antonio

AU - Aiuti, Fernando

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Increased levels of serum IgE and eosinophilia have been described in human immunodeficiency virus (HIV) infection, almost exclusively in patients with CD4+ cell count + T helper type 2 (Th-2) lymphocytes, producing interleukin 4 (IL-4) and expressing a ligand for the B cell-specific CD40 molecule (CD40 ligand [L]). A shift to a Th-2-like pattern of cytokine secretion has been postulated to be associated with progression toward acquired immunodeficiency syndrome (AIDS). We studied three AIDS patients with very high levels of IgE and almost complete depletion of CD4+ lymphocytes, suggesting that IgE synthesis could not be driven by CD4+ cells. IgE in vitro synthesis by cells from such patients was, however, inhibited by anti-IL-4. We show that both CD8+ T cell lines and majority of CD8+ T cell clones derived from these patients produce IL-4, IL-5, and IL-6 in half of the cases together with interferon γ (IFN- γ). 44% of CD8- T cell clones expressed a CD40L, and the supernatants of the clones were capable of inducing IgE synthesis by B cells costimulated with anti-CD40. CD8+ T cells in these patients therefore functionally mimic Th-2 type cells and may account for hyper-IgE and eosinophilia in the absence of CD4+ cells. The presence of such CD8+ cells may also provide a source of IL-4 directing the development of predominant Th-2 responses in HIV infection.

AB - Increased levels of serum IgE and eosinophilia have been described in human immunodeficiency virus (HIV) infection, almost exclusively in patients with CD4+ cell count + T helper type 2 (Th-2) lymphocytes, producing interleukin 4 (IL-4) and expressing a ligand for the B cell-specific CD40 molecule (CD40 ligand [L]). A shift to a Th-2-like pattern of cytokine secretion has been postulated to be associated with progression toward acquired immunodeficiency syndrome (AIDS). We studied three AIDS patients with very high levels of IgE and almost complete depletion of CD4+ lymphocytes, suggesting that IgE synthesis could not be driven by CD4+ cells. IgE in vitro synthesis by cells from such patients was, however, inhibited by anti-IL-4. We show that both CD8+ T cell lines and majority of CD8+ T cell clones derived from these patients produce IL-4, IL-5, and IL-6 in half of the cases together with interferon γ (IFN- γ). 44% of CD8- T cell clones expressed a CD40L, and the supernatants of the clones were capable of inducing IgE synthesis by B cells costimulated with anti-CD40. CD8+ T cells in these patients therefore functionally mimic Th-2 type cells and may account for hyper-IgE and eosinophilia in the absence of CD4+ cells. The presence of such CD8+ cells may also provide a source of IL-4 directing the development of predominant Th-2 responses in HIV infection.

UR - http://www.scopus.com/inward/record.url?scp=0028959166&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028959166&partnerID=8YFLogxK

U2 - 10.1084/jem.181.1.423

DO - 10.1084/jem.181.1.423

M3 - Article

VL - 181

SP - 423

EP - 428

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 1

ER -