CD8+CD11b+ peripheral blood T lymphocytes contain lymphokine-activated killer cell precursors

U. Dianzani, D. Zarcone, V. Pistoia, C. E. Grossi, A. Pileri, M. Massaia, M. Ferrarini

Research output: Contribution to journalArticle

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Abstract

CD3+CD8+ cells, purified from peripheral blood T cells by depletion of CD4+ lymphocytes, were tested for their cytotoxic activity against K-562 or HL-60 cells. Freshly prepared cells had no cytotoxic function, but upon exposure to recombinant interleukin 2 (rIL2) in vitro they acquired a lymphokine-activated killer (LAK) activity. Fractionation of CD3+CD8+ cells into CD11b+ and CD11b- cells demonstrated that all the cells with the potential of developing LAK cell functions were within the CD8+CD11b+ subset. These cells lacked natural killer cell markers such as CD16 or NKH1, but a proportion of them stained for Leu-7. Furthermore, they expressed the α/β chains, but not the γ/δ chains, of the T cell receptor, as could be determined by staining with the appropriate monoclonal antibodies. CD8+CD11b+ cells had a large granular lymphocyte morphology, as shown by both cytochemical and electron microscopy analyses. They proliferated in response to IL2 in vitro and developed cytotoxic functions against a number of natural killer resistant targets. Their response to phytohemagglutinin or pokeweed mitogen was very weak or absent. By contrast, CD8+CD11b- cells failed to generate LAK cells in response to rIL2, did not show a large granular lymphocyte morphology, but responded vigorously to phytohemagglutinin or pokeweek mitogen. Purified CD8+CD11b+ cells were cloned by limiting dilution in the presence of rIL2. The observed cloning efficiency of 19 ± 0.3% indicates that a fraction of the cells only could respond to IL2. Furthermore, only 50% of the clones obtained had a LAK cell function. These data show that CD8+CD11b+ cells represent a heterogeneous cell population. Nevertheless this cell subset probably represents the major source of LAK cell progenitors within the circulating T cells.

Original languageEnglish
Pages (from-to)1037-1044
Number of pages8
JournalEuropean Journal of Immunology
Volume19
Issue number6
Publication statusPublished - 1989

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Lymphokine-Activated Killer Cells
T-Lymphocytes
Interleukin-2
Phytohemagglutinins
Lymphocytes
Lymphocyte Depletion
Pokeweed Mitogens
Lymphokines
HL-60 Cells
T-Cell Antigen Receptor
Mitogens
Natural Killer Cells
Organism Cloning

ASJC Scopus subject areas

  • Immunology

Cite this

Dianzani, U., Zarcone, D., Pistoia, V., Grossi, C. E., Pileri, A., Massaia, M., & Ferrarini, M. (1989). CD8+CD11b+ peripheral blood T lymphocytes contain lymphokine-activated killer cell precursors. European Journal of Immunology, 19(6), 1037-1044.

CD8+CD11b+ peripheral blood T lymphocytes contain lymphokine-activated killer cell precursors. / Dianzani, U.; Zarcone, D.; Pistoia, V.; Grossi, C. E.; Pileri, A.; Massaia, M.; Ferrarini, M.

In: European Journal of Immunology, Vol. 19, No. 6, 1989, p. 1037-1044.

Research output: Contribution to journalArticle

Dianzani, U, Zarcone, D, Pistoia, V, Grossi, CE, Pileri, A, Massaia, M & Ferrarini, M 1989, 'CD8+CD11b+ peripheral blood T lymphocytes contain lymphokine-activated killer cell precursors', European Journal of Immunology, vol. 19, no. 6, pp. 1037-1044.
Dianzani, U. ; Zarcone, D. ; Pistoia, V. ; Grossi, C. E. ; Pileri, A. ; Massaia, M. ; Ferrarini, M. / CD8+CD11b+ peripheral blood T lymphocytes contain lymphokine-activated killer cell precursors. In: European Journal of Immunology. 1989 ; Vol. 19, No. 6. pp. 1037-1044.
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abstract = "CD3+CD8+ cells, purified from peripheral blood T cells by depletion of CD4+ lymphocytes, were tested for their cytotoxic activity against K-562 or HL-60 cells. Freshly prepared cells had no cytotoxic function, but upon exposure to recombinant interleukin 2 (rIL2) in vitro they acquired a lymphokine-activated killer (LAK) activity. Fractionation of CD3+CD8+ cells into CD11b+ and CD11b- cells demonstrated that all the cells with the potential of developing LAK cell functions were within the CD8+CD11b+ subset. These cells lacked natural killer cell markers such as CD16 or NKH1, but a proportion of them stained for Leu-7. Furthermore, they expressed the α/β chains, but not the γ/δ chains, of the T cell receptor, as could be determined by staining with the appropriate monoclonal antibodies. CD8+CD11b+ cells had a large granular lymphocyte morphology, as shown by both cytochemical and electron microscopy analyses. They proliferated in response to IL2 in vitro and developed cytotoxic functions against a number of natural killer resistant targets. Their response to phytohemagglutinin or pokeweed mitogen was very weak or absent. By contrast, CD8+CD11b- cells failed to generate LAK cells in response to rIL2, did not show a large granular lymphocyte morphology, but responded vigorously to phytohemagglutinin or pokeweek mitogen. Purified CD8+CD11b+ cells were cloned by limiting dilution in the presence of rIL2. The observed cloning efficiency of 19 ± 0.3{\%} indicates that a fraction of the cells only could respond to IL2. Furthermore, only 50{\%} of the clones obtained had a LAK cell function. These data show that CD8+CD11b+ cells represent a heterogeneous cell population. Nevertheless this cell subset probably represents the major source of LAK cell progenitors within the circulating T cells.",
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AU - Ferrarini, M.

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