CD8+CD28- T lymphocytes from HIV-1-infected patients secrete factors that induce endothelial cell proliferation and acquisition of Kaposi's sarcoma cell features

Giulio Alessandri, Simona Fiorentini, Stefano Licenziati, Monica Bonafede, Paolo Monini, Barbara Ensoli, Arnaldo Caruso

Research output: Contribution to journalArticle

Abstract

Kaposi's sarcoma (KS) develops more frequently in human immunodeficiency virus type 1 (HIV-1)-infected patients. In this study, we report that molecules released by CD8+CD28- T lymphocytes from HIV-1-infected patients promote endothelial-cell (EC) growth and induce ECs to acquire spindle cell morphology and upregulation of intercellular adhesion molecule-1 (ICAM-1), E-selectin, and vascular endothelial cell growth factor receptor-3 (VEGFR-3) (a typical feature of the KS cell phenotype). The effects observed on ECs cocultured with in vivo activated CD28- cells were partly reproduced when ECs were grown in medium containing interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α). At concentrations similar to those found in the supernatant of in vivo activated CD28- cells, the two proinflammatory cytokines sustained EC growth and survival only when combined. We, therefore, conclude that CD28- T lymphocytes from HIV-1-infected patients exert their effect on ECs through a mechanism involving both IFN-γ and TNF-α. This finding may have wide implications for our basic understanding of the immunopathology of KS.

Original languageEnglish
Pages (from-to)523-531
Number of pages9
JournalJournal of Interferon and Cytokine Research
Volume23
Issue number9
DOIs
Publication statusPublished - Sep 1 2003

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Kaposi's Sarcoma
HIV-1
Endothelial Cells
Cell Proliferation
T-Lymphocytes
Interferons
Tumor Necrosis Factor-alpha
Vascular Endothelial Growth Factor Receptor-3
Vascular Endothelial Growth Factor Receptor
E-Selectin
Intercellular Adhesion Molecule-1
Growth
Cell Survival
Up-Regulation
Cytokines
Phenotype

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Cell Biology

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CD8+CD28- T lymphocytes from HIV-1-infected patients secrete factors that induce endothelial cell proliferation and acquisition of Kaposi's sarcoma cell features. / Alessandri, Giulio; Fiorentini, Simona; Licenziati, Stefano; Bonafede, Monica; Monini, Paolo; Ensoli, Barbara; Caruso, Arnaldo.

In: Journal of Interferon and Cytokine Research, Vol. 23, No. 9, 01.09.2003, p. 523-531.

Research output: Contribution to journalArticle

Alessandri, Giulio ; Fiorentini, Simona ; Licenziati, Stefano ; Bonafede, Monica ; Monini, Paolo ; Ensoli, Barbara ; Caruso, Arnaldo. / CD8+CD28- T lymphocytes from HIV-1-infected patients secrete factors that induce endothelial cell proliferation and acquisition of Kaposi's sarcoma cell features. In: Journal of Interferon and Cytokine Research. 2003 ; Vol. 23, No. 9. pp. 523-531.
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