CD94 functions as a natural killer cell inhibitory receptor for different HLA class I alleles: Identification of the inhibitory form of CD94 by the use of novel monoclonal antibodies

Simona Sivori, Massimo Vitale, Cristina Bottino, Emanuela Marcenaro, Lorenza Sanseverino, Silvia Parolini, Lorenzo Moretta, Alessandro Moretta

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

CD94 molecules have been suggested to function as inhibitory natural killer cell (NK) receptors involved in the recognition of HLA-B alleles sharing the Bw6 supertypic specificity. In this study, we show that CD94 molecules may play a more general role: they are also involved in the recognition of other HLA class I molecules, including HLA-C and at least some HLA-A alleles. The inhibitory effect mediated by CD94 molecules on NK cytolytic activity is lower in magnitude than that of bona fide inhibitory receptors such as p58 or p70. Distinct from the other human NK receptors involved in HLA class I recognition, CD94 is expressed on virtually all NK cells. In addition, it has been shown to be functionally heterogeneous since, in different clones, CD94 mediated either cell triggering or inhibition. Although NK cells expressing inhibitory CD94 molecules are usually characterized by a CD94(bright) phenotype, there is no precise correlation between fluorescence intensity and inhibitory or activating function. Here, we describe two novel monoclonal antibodies (mAb) which selectively recognize inhibitory CD94 molecules and bind to a subset (variable in size among different donors) of CD94(bright) cells. The use of these mAb allows the direct assessment of NK cells expressing inhibitory CD94 receptors both at the population and at the clonal level.

Original languageEnglish
Pages (from-to)2487-2492
Number of pages6
JournalEuropean Journal of Immunology
Volume26
Issue number10
DOIs
Publication statusPublished - 1996

Fingerprint

Natural Killer Cell Receptors
Natural Killer Cells
Alleles
Monoclonal Antibodies
HLA-C Antigens
HLA-A Antigens
HLA-B Antigens
Clone Cells
Fluorescence
Phenotype
Population

Keywords

  • CD94
  • Cytotoxicity
  • Natural killer cell receptor

ASJC Scopus subject areas

  • Immunology

Cite this

CD94 functions as a natural killer cell inhibitory receptor for different HLA class I alleles : Identification of the inhibitory form of CD94 by the use of novel monoclonal antibodies. / Sivori, Simona; Vitale, Massimo; Bottino, Cristina; Marcenaro, Emanuela; Sanseverino, Lorenza; Parolini, Silvia; Moretta, Lorenzo; Moretta, Alessandro.

In: European Journal of Immunology, Vol. 26, No. 10, 1996, p. 2487-2492.

Research output: Contribution to journalArticle

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