CD94/NKG2 Inhibitory Receptor Complex Modulates Both Anti-Viral and Anti-Tumoral Responses of Polyclonal Phosphoantigen-Reactive Vγ9Vδ2 T Lymphocytes

Fabrizio Poccia, Barbara Cipriani, Silvia Vendetti, Vittorio Colizzi, Yannick Poquet, Luca Battistini, Miguel López-Botet, Jean Jacques Fournié, Marie Lise Gougeon

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Viral, bacterial, protozoal, and cancer-associated Ags elicit strong responses in human γδ T lymphocytes. The majority of these cells in the peripheral blood express the Vγ9Vδ2-encoded TCR and recognize nonpeptidic phosphoantigens without an apparent MHC restriction. We have shown that Vγ9Vδ2 T cells express the inhibitory CD94/NKG2 receptor for HLA class I molecules The anti-CD94 mAb inhibits 1) the Vγ9Vδ2 T cell proliferation in response mycobacterial phosphoantigens and 2) the HIV-induced Vγ9Vδ2 T cell expansion. Vγ9Vδ2 T cells stimulated with nonpeptidic mycobacterial antigens produce IFN-γ and TNF-α. Signaling through the CD94/NKG2 receptor interferes with the synthesis of these cytokines. The CD94/HLA class I interaction is also involved in the cytotoxic activity of Vγ9Vδ2 T cells. The Vγ9Vδ2 T cell regulation through the CD94 receptor may be important for the potentially dual function in innate immunity, i.e., 1) NK-like and 2) TCR ligand-induced cytolytic activities.

Original languageEnglish
Pages (from-to)6009-6017
Number of pages9
JournalJournal of Immunology
Volume159
Issue number12
Publication statusPublished - Dec 15 1997

Fingerprint

T-Lymphocytes
HIV-2
Innate Immunity
Cell Proliferation
Cytokines
Ligands
Antigens
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

CD94/NKG2 Inhibitory Receptor Complex Modulates Both Anti-Viral and Anti-Tumoral Responses of Polyclonal Phosphoantigen-Reactive Vγ9Vδ2 T Lymphocytes. / Poccia, Fabrizio; Cipriani, Barbara; Vendetti, Silvia; Colizzi, Vittorio; Poquet, Yannick; Battistini, Luca; López-Botet, Miguel; Fournié, Jean Jacques; Gougeon, Marie Lise.

In: Journal of Immunology, Vol. 159, No. 12, 15.12.1997, p. 6009-6017.

Research output: Contribution to journalArticle

Poccia, F, Cipriani, B, Vendetti, S, Colizzi, V, Poquet, Y, Battistini, L, López-Botet, M, Fournié, JJ & Gougeon, ML 1997, 'CD94/NKG2 Inhibitory Receptor Complex Modulates Both Anti-Viral and Anti-Tumoral Responses of Polyclonal Phosphoantigen-Reactive Vγ9Vδ2 T Lymphocytes', Journal of Immunology, vol. 159, no. 12, pp. 6009-6017.
Poccia, Fabrizio ; Cipriani, Barbara ; Vendetti, Silvia ; Colizzi, Vittorio ; Poquet, Yannick ; Battistini, Luca ; López-Botet, Miguel ; Fournié, Jean Jacques ; Gougeon, Marie Lise. / CD94/NKG2 Inhibitory Receptor Complex Modulates Both Anti-Viral and Anti-Tumoral Responses of Polyclonal Phosphoantigen-Reactive Vγ9Vδ2 T Lymphocytes. In: Journal of Immunology. 1997 ; Vol. 159, No. 12. pp. 6009-6017.
@article{667377084e3a4a5f8dc323e867f46175,
title = "CD94/NKG2 Inhibitory Receptor Complex Modulates Both Anti-Viral and Anti-Tumoral Responses of Polyclonal Phosphoantigen-Reactive Vγ9Vδ2 T Lymphocytes",
abstract = "Viral, bacterial, protozoal, and cancer-associated Ags elicit strong responses in human γδ T lymphocytes. The majority of these cells in the peripheral blood express the Vγ9Vδ2-encoded TCR and recognize nonpeptidic phosphoantigens without an apparent MHC restriction. We have shown that Vγ9Vδ2 T cells express the inhibitory CD94/NKG2 receptor for HLA class I molecules The anti-CD94 mAb inhibits 1) the Vγ9Vδ2 T cell proliferation in response mycobacterial phosphoantigens and 2) the HIV-induced Vγ9Vδ2 T cell expansion. Vγ9Vδ2 T cells stimulated with nonpeptidic mycobacterial antigens produce IFN-γ and TNF-α. Signaling through the CD94/NKG2 receptor interferes with the synthesis of these cytokines. The CD94/HLA class I interaction is also involved in the cytotoxic activity of Vγ9Vδ2 T cells. The Vγ9Vδ2 T cell regulation through the CD94 receptor may be important for the potentially dual function in innate immunity, i.e., 1) NK-like and 2) TCR ligand-induced cytolytic activities.",
author = "Fabrizio Poccia and Barbara Cipriani and Silvia Vendetti and Vittorio Colizzi and Yannick Poquet and Luca Battistini and Miguel L{\'o}pez-Botet and Fourni{\'e}, {Jean Jacques} and Gougeon, {Marie Lise}",
year = "1997",
month = "12",
day = "15",
language = "English",
volume = "159",
pages = "6009--6017",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "12",

}

TY - JOUR

T1 - CD94/NKG2 Inhibitory Receptor Complex Modulates Both Anti-Viral and Anti-Tumoral Responses of Polyclonal Phosphoantigen-Reactive Vγ9Vδ2 T Lymphocytes

AU - Poccia, Fabrizio

AU - Cipriani, Barbara

AU - Vendetti, Silvia

AU - Colizzi, Vittorio

AU - Poquet, Yannick

AU - Battistini, Luca

AU - López-Botet, Miguel

AU - Fournié, Jean Jacques

AU - Gougeon, Marie Lise

PY - 1997/12/15

Y1 - 1997/12/15

N2 - Viral, bacterial, protozoal, and cancer-associated Ags elicit strong responses in human γδ T lymphocytes. The majority of these cells in the peripheral blood express the Vγ9Vδ2-encoded TCR and recognize nonpeptidic phosphoantigens without an apparent MHC restriction. We have shown that Vγ9Vδ2 T cells express the inhibitory CD94/NKG2 receptor for HLA class I molecules The anti-CD94 mAb inhibits 1) the Vγ9Vδ2 T cell proliferation in response mycobacterial phosphoantigens and 2) the HIV-induced Vγ9Vδ2 T cell expansion. Vγ9Vδ2 T cells stimulated with nonpeptidic mycobacterial antigens produce IFN-γ and TNF-α. Signaling through the CD94/NKG2 receptor interferes with the synthesis of these cytokines. The CD94/HLA class I interaction is also involved in the cytotoxic activity of Vγ9Vδ2 T cells. The Vγ9Vδ2 T cell regulation through the CD94 receptor may be important for the potentially dual function in innate immunity, i.e., 1) NK-like and 2) TCR ligand-induced cytolytic activities.

AB - Viral, bacterial, protozoal, and cancer-associated Ags elicit strong responses in human γδ T lymphocytes. The majority of these cells in the peripheral blood express the Vγ9Vδ2-encoded TCR and recognize nonpeptidic phosphoantigens without an apparent MHC restriction. We have shown that Vγ9Vδ2 T cells express the inhibitory CD94/NKG2 receptor for HLA class I molecules The anti-CD94 mAb inhibits 1) the Vγ9Vδ2 T cell proliferation in response mycobacterial phosphoantigens and 2) the HIV-induced Vγ9Vδ2 T cell expansion. Vγ9Vδ2 T cells stimulated with nonpeptidic mycobacterial antigens produce IFN-γ and TNF-α. Signaling through the CD94/NKG2 receptor interferes with the synthesis of these cytokines. The CD94/HLA class I interaction is also involved in the cytotoxic activity of Vγ9Vδ2 T cells. The Vγ9Vδ2 T cell regulation through the CD94 receptor may be important for the potentially dual function in innate immunity, i.e., 1) NK-like and 2) TCR ligand-induced cytolytic activities.

UR - http://www.scopus.com/inward/record.url?scp=0031573616&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031573616&partnerID=8YFLogxK

M3 - Article

VL - 159

SP - 6009

EP - 6017

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 12

ER -