Cdc42 is involved in basal cell carcinoma carcinogenesis

Maria Giovanna Tucci, Guendalina Lucarini, Antonio Zizzi, Romina Rocchetti, Donatella Brancorsini, Roberto Di Primio, Francesca Ricotti, Giuseppe Ricotti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Basal cell carcinoma (BCC) is the most common type of skin cancer in older persons and is a rapidly rising incidence. E-cadherin-mediated cell-cell adhesion activates Cdc42, a Rho GTPase essential for cell polarity in numerous settings. No study has yet addressed a biological significance of Cdc42 alterations in BCC pathogenesis. Our aim was to investigate E-cadherin-dependent cell-cell contacts and Cdc42 activity in BCC formation. We evaluated E-cadherin and Cdc42 expression by immunohistochemistry and Western blot analysis in samples of 15 normal skin (NS) and 30 BCC (10 superficial, 9 nodular and 11 infiltrative subtypes). Low E-cadherin and high Cdc42 immunohistochemical expression were found in BCC samples compared with NS. E-cadherin staining was significantly reduced in infiltrative BCC compared with superficial and nodular. A significantly greater Cdc42 expression was observed in BCC compared with NS; moreover, superficial BCC had a significantly lower Cdc42 expression in respect to the other subtypes. Western blot analysis confirmed the significantly decreased E-cadherin expression in infiltrative BCC as well as Cdc42 reduction in superficial BCC in respect to the other subtypes. In BCC the increased Cdc42 in association with reduced E-cadherin might contribute to the disruption of adhesion mechanisms and to the loss of cell polarity, thus explaining a mechanism by which cancer cells can escape from the control of adjacent normal keratinocytes. Our study also showed that Cdc42 and E-cadherin expression differed according to aggressive behaviour of BCC subtypes and suggested important functions of these molecules in regulating tumour demarcation and progression.

Original languageEnglish
Pages (from-to)835-840
Number of pages6
JournalArchives of Dermatological Research
Volume305
Issue number9
DOIs
Publication statusPublished - Nov 2013

Fingerprint

Basal Cell Carcinoma
Carcinogenesis
Cadherins
Cell Polarity
Skin
Western Blotting
rho GTP-Binding Proteins
Skin Neoplasms
Keratinocytes
Cell Adhesion
Neoplasms
Immunohistochemistry
Staining and Labeling

Keywords

  • Basal cell carcinoma
  • Cdc42
  • Cell polarity
  • E-cadherin

ASJC Scopus subject areas

  • Dermatology

Cite this

Tucci, M. G., Lucarini, G., Zizzi, A., Rocchetti, R., Brancorsini, D., Primio, R. D., ... Ricotti, G. (2013). Cdc42 is involved in basal cell carcinoma carcinogenesis. Archives of Dermatological Research, 305(9), 835-840. https://doi.org/10.1007/s00403-013-1351-8

Cdc42 is involved in basal cell carcinoma carcinogenesis. / Tucci, Maria Giovanna; Lucarini, Guendalina; Zizzi, Antonio; Rocchetti, Romina; Brancorsini, Donatella; Primio, Roberto Di; Ricotti, Francesca; Ricotti, Giuseppe.

In: Archives of Dermatological Research, Vol. 305, No. 9, 11.2013, p. 835-840.

Research output: Contribution to journalArticle

Tucci, MG, Lucarini, G, Zizzi, A, Rocchetti, R, Brancorsini, D, Primio, RD, Ricotti, F & Ricotti, G 2013, 'Cdc42 is involved in basal cell carcinoma carcinogenesis', Archives of Dermatological Research, vol. 305, no. 9, pp. 835-840. https://doi.org/10.1007/s00403-013-1351-8
Tucci MG, Lucarini G, Zizzi A, Rocchetti R, Brancorsini D, Primio RD et al. Cdc42 is involved in basal cell carcinoma carcinogenesis. Archives of Dermatological Research. 2013 Nov;305(9):835-840. https://doi.org/10.1007/s00403-013-1351-8
Tucci, Maria Giovanna ; Lucarini, Guendalina ; Zizzi, Antonio ; Rocchetti, Romina ; Brancorsini, Donatella ; Primio, Roberto Di ; Ricotti, Francesca ; Ricotti, Giuseppe. / Cdc42 is involved in basal cell carcinoma carcinogenesis. In: Archives of Dermatological Research. 2013 ; Vol. 305, No. 9. pp. 835-840.
@article{18e0429e47664abeb6878eaeb997cfac,
title = "Cdc42 is involved in basal cell carcinoma carcinogenesis",
abstract = "Basal cell carcinoma (BCC) is the most common type of skin cancer in older persons and is a rapidly rising incidence. E-cadherin-mediated cell-cell adhesion activates Cdc42, a Rho GTPase essential for cell polarity in numerous settings. No study has yet addressed a biological significance of Cdc42 alterations in BCC pathogenesis. Our aim was to investigate E-cadherin-dependent cell-cell contacts and Cdc42 activity in BCC formation. We evaluated E-cadherin and Cdc42 expression by immunohistochemistry and Western blot analysis in samples of 15 normal skin (NS) and 30 BCC (10 superficial, 9 nodular and 11 infiltrative subtypes). Low E-cadherin and high Cdc42 immunohistochemical expression were found in BCC samples compared with NS. E-cadherin staining was significantly reduced in infiltrative BCC compared with superficial and nodular. A significantly greater Cdc42 expression was observed in BCC compared with NS; moreover, superficial BCC had a significantly lower Cdc42 expression in respect to the other subtypes. Western blot analysis confirmed the significantly decreased E-cadherin expression in infiltrative BCC as well as Cdc42 reduction in superficial BCC in respect to the other subtypes. In BCC the increased Cdc42 in association with reduced E-cadherin might contribute to the disruption of adhesion mechanisms and to the loss of cell polarity, thus explaining a mechanism by which cancer cells can escape from the control of adjacent normal keratinocytes. Our study also showed that Cdc42 and E-cadherin expression differed according to aggressive behaviour of BCC subtypes and suggested important functions of these molecules in regulating tumour demarcation and progression.",
keywords = "Basal cell carcinoma, Cdc42, Cell polarity, E-cadherin",
author = "Tucci, {Maria Giovanna} and Guendalina Lucarini and Antonio Zizzi and Romina Rocchetti and Donatella Brancorsini and Primio, {Roberto Di} and Francesca Ricotti and Giuseppe Ricotti",
year = "2013",
month = "11",
doi = "10.1007/s00403-013-1351-8",
language = "English",
volume = "305",
pages = "835--840",
journal = "Archives of Dermatological Research",
issn = "0340-3696",
publisher = "Springer Verlag",
number = "9",

}

TY - JOUR

T1 - Cdc42 is involved in basal cell carcinoma carcinogenesis

AU - Tucci, Maria Giovanna

AU - Lucarini, Guendalina

AU - Zizzi, Antonio

AU - Rocchetti, Romina

AU - Brancorsini, Donatella

AU - Primio, Roberto Di

AU - Ricotti, Francesca

AU - Ricotti, Giuseppe

PY - 2013/11

Y1 - 2013/11

N2 - Basal cell carcinoma (BCC) is the most common type of skin cancer in older persons and is a rapidly rising incidence. E-cadherin-mediated cell-cell adhesion activates Cdc42, a Rho GTPase essential for cell polarity in numerous settings. No study has yet addressed a biological significance of Cdc42 alterations in BCC pathogenesis. Our aim was to investigate E-cadherin-dependent cell-cell contacts and Cdc42 activity in BCC formation. We evaluated E-cadherin and Cdc42 expression by immunohistochemistry and Western blot analysis in samples of 15 normal skin (NS) and 30 BCC (10 superficial, 9 nodular and 11 infiltrative subtypes). Low E-cadherin and high Cdc42 immunohistochemical expression were found in BCC samples compared with NS. E-cadherin staining was significantly reduced in infiltrative BCC compared with superficial and nodular. A significantly greater Cdc42 expression was observed in BCC compared with NS; moreover, superficial BCC had a significantly lower Cdc42 expression in respect to the other subtypes. Western blot analysis confirmed the significantly decreased E-cadherin expression in infiltrative BCC as well as Cdc42 reduction in superficial BCC in respect to the other subtypes. In BCC the increased Cdc42 in association with reduced E-cadherin might contribute to the disruption of adhesion mechanisms and to the loss of cell polarity, thus explaining a mechanism by which cancer cells can escape from the control of adjacent normal keratinocytes. Our study also showed that Cdc42 and E-cadherin expression differed according to aggressive behaviour of BCC subtypes and suggested important functions of these molecules in regulating tumour demarcation and progression.

AB - Basal cell carcinoma (BCC) is the most common type of skin cancer in older persons and is a rapidly rising incidence. E-cadherin-mediated cell-cell adhesion activates Cdc42, a Rho GTPase essential for cell polarity in numerous settings. No study has yet addressed a biological significance of Cdc42 alterations in BCC pathogenesis. Our aim was to investigate E-cadherin-dependent cell-cell contacts and Cdc42 activity in BCC formation. We evaluated E-cadherin and Cdc42 expression by immunohistochemistry and Western blot analysis in samples of 15 normal skin (NS) and 30 BCC (10 superficial, 9 nodular and 11 infiltrative subtypes). Low E-cadherin and high Cdc42 immunohistochemical expression were found in BCC samples compared with NS. E-cadherin staining was significantly reduced in infiltrative BCC compared with superficial and nodular. A significantly greater Cdc42 expression was observed in BCC compared with NS; moreover, superficial BCC had a significantly lower Cdc42 expression in respect to the other subtypes. Western blot analysis confirmed the significantly decreased E-cadherin expression in infiltrative BCC as well as Cdc42 reduction in superficial BCC in respect to the other subtypes. In BCC the increased Cdc42 in association with reduced E-cadherin might contribute to the disruption of adhesion mechanisms and to the loss of cell polarity, thus explaining a mechanism by which cancer cells can escape from the control of adjacent normal keratinocytes. Our study also showed that Cdc42 and E-cadherin expression differed according to aggressive behaviour of BCC subtypes and suggested important functions of these molecules in regulating tumour demarcation and progression.

KW - Basal cell carcinoma

KW - Cdc42

KW - Cell polarity

KW - E-cadherin

UR - http://www.scopus.com/inward/record.url?scp=84886717196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886717196&partnerID=8YFLogxK

U2 - 10.1007/s00403-013-1351-8

DO - 10.1007/s00403-013-1351-8

M3 - Article

C2 - 23589095

AN - SCOPUS:84886717196

VL - 305

SP - 835

EP - 840

JO - Archives of Dermatological Research

JF - Archives of Dermatological Research

SN - 0340-3696

IS - 9

ER -